Animal Genetics
Genetic analysis of five sedentary fish species in middle Laranjinha River (upper Paran�¡ River basin): A case study
Authors: W. Frantine-Silva, D.G. Ferreira, R.H.C. Nascimento, J.F. Fracasso, J.E. Conte, F.P. Ramos, S. Carvalho and B.A. Galindo
Most studies of diversity and genetic structure in neotropical fish have focused on commercial species from large rivers or their reservoirs. However, smaller tributaries have been identified as an important alternative migratory route, with independent pools of genetic diversity. In this context, the present study aimed to evaluate genetic diversity and .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.13
- DOI:
- 10.4238/2015.December.28.13
High polymorphism at microsatellite loci in the Chinese donkey
Authors: R.F. Zhang, W.M. Xie, T. Zhang and C.Z. Lei
To reveal the genetic diversity and phylogenetic relationships between Chinese donkey breeds, 415 individuals representing ten breeds were investigated using ten microsatellite markers. The observed number of alleles, mean effective number of alleles (NE), mean expected heterozygosity (HE), and polymorphic information content .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028291
- DOI:
- 10.4238/gmr.15028291
Screening of genes related to ovarian development in the swimming crab, Portunus trituberculatus, by suppression subtractive hybridization
Authors: Z.B. Yu, C.K. Mu, W.W. Song, R.H. Li, Y.E. Chen and C.L. Wang
The swimming crab, Portunus trituberculatus, is an important marine animal and is widely cultured in China. In the present study, suppression subtractive hybridization was applied to identify the differentially expressed genes in the ovaries of mature and immature P. trituberculatus. One hundred and seventy six expressed sequence tag (ESTs) were identifie.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.16
- DOI:
- 10.4238/2015.December.28.16
Polymorphisms in GJA1 and their association with growth traits in chicken
Authors: M. Shahjahan, R.R. Liu, G.P. Zhao, J.J. Zhang, M.Q. Zheng, Q.H. Li and J. Wen
This study aimed to screen single nucleotide polymorphisms (SNPs) in the chicken gap junction protein alpha 1 (GJA1) gene, and to investigate their association with five growth traits measured in 269 chickens encompassing Chinese indigenous Beijing-You (BJY) and commercial Cobb broiler (CB) populations. Four variants were detected in the chicken GJA1 gene.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.33
- DOI:
- 10.4238/2015.December.28.33
Rapid and sensitive LC-MS/MS method for the determination of auraptene in rat plasma and its application in a pharmacokinetic and bioavailability study in rats
Authors: X.D. Ye, H. Ouyang, L.Y. Zhong, T.E. Li, X.Y. Rao, Y.L. Feng and W.L. Yang
A simple, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of auraptene, a constituent isolated from Fructus aurantii with potential to combat Alzheimer’s disease, in rat plasma. Rat plasma samples were pretreated by protein precipitation with .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028786
- DOI:
- 10.4238/gmr.15028786
Preparation of polyclonal antibody against porcine beta defensin 2 and identification of its distribution in tissues of pig
Authors: Y.Y. Bao, L. Li, H. Zhang, C.Y. Gao, C.B. Xiao and C.L. Li
Porcine β-defensin 2 (pBD2) is an antimicrobial peptide in pigs that plays an important role in the immune system by preventing bacterial invasion. To produce an anti-pBD2 antibody, which is not commercially available, we expressed and purified a soluble, his-tagged version of pBD2 (his-pBD2). Purified pBD2 was injected into New Zealand white rabbits.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.36
- DOI:
- 10.4238/2015.December.28.36
Comparative transcriptome analysis of testes and ovaries for the discovery of novel genes from Amur sturgeon (Acipenser schrenckii)
Authors: S.B. Jin, Y. Zhang, X.L. Dong, Q.K. Xi, D. Song, H.T. Fu and D.J. Sun
Sturgeons (Acipenser schrenckii) are of high evolutionary, economic, and conservation value, and caviar isone of the most valuable animal food products in the world. The Illumina HiSeq2000 sequencing platform was used to construct testicular and ovarian transcriptomes to identify genes involved in reproduction and sex determination in A. schrenckii. A tot.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.40
- DOI:
- 10.4238/2015.December.28.40
In vivo determination of muscle-derived stem cells in rat corpus cavernosum
Authors: L.J. Xu, B.X. Xue, Y.X. Shan, D. Chen, J. Gao, D.R. Yang, C.Y. Sun and Y. Cui
The aim of this in vivo study was to determine the existence of muscle-derived stem cells (MDSCs) in rat corpus cavernosum. Immunohistochemical and RT-PCR analyses were performed to determine the expression of the stem cell markers (Sca-1, Oct4, and desmin) in Sprague-Dawley (SD) rats in different age groups (10 rats in each group). Sca-1 was mainly expre.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.1
- DOI:
- 10.4238/2015.August.21.1
Transcriptomic differences of genes in the avian target of rapamycin (avTOR) pathway in a divergent line of meat-type chickens selected for feed efficiency
Authors: J. Lee and S.E. Aggrey
Avian target of rapamycin (avTOR) is a highly conserved serine-threonine kinase that serves as an intracellular energy and nutrient sensor and regulates cell division, growth, and apoptosis. The role of avTOR in mediating feed intake and growth in poultry is unknown. We studied avTOR signaling activities in duodenum and liver .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027120
- DOI:
- 10.4238/gmr.15027120
A single nucleotide polymorphism in NEUROD1 is associated with production traits in Nelore beef cattle
Authors: P.S.N. de Oliveira, P.C. Tizioto, W. Malago Jr, M.L. do Nascimento, A.S.M. Cesar, W.J.S. Diniz, M.M. de Souza, D.P.D. Lanna, R.R. Tullio, G.B. Mour�£o, M. de A. Mudadu, L.L. Coutinho and L.C. de A. Regitano
Feed efficiency and carcass characteristics are late-measured traits. The detection of molecular markers associated with them can help breeding programs to select animals early in life, and to predict breeding values with high accuracy. The objective of this study was to identify polymorphisms in the functional and positional .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028161
- DOI:
- 10.4238/gmr.15028161