Animal Genetics

Polymorphisms in the GHRL gene and their associations with traits of economic interest in beef cattle

Authors: C.U. Braz, G.M.F. Camargo, D.F. Cardoso, F.M.M. Gil, P.D.S. Fonseca, J.N.S.G. Cyrillo, M.E.Z. Mercadante, H.N. Oliveira and H. Tonhati

The hormone ghrelin is produced in the stomach wall, has an orexigenic function, stimulates growth hormone secretion, and affects the energy balance of the animal. Therefore, the ghrelin gene (GHRL) is considered to be a good candidate marker for the identification of traits of great economic importance in cattle, such as those associated with feed intake.. Read More»

Genet. Mol. Res. 14(4):

2015.December.23.6

DOI:

10.4238/2015.December.23.6

Expression of N-cadherin in myocardial tissues during the development of a rat heart

Authors: L.M. Mu, W.F. Wang, H. Zheng, Z.K. Guo and G.M. Zhang

We investigated the expression and distribution of N-cadherin during the development of a rat heart. Immunohistochemistry (IHC) was performed to detect the expression and distribution of N-cadherin in the myocardial tissues of rats at embryonic day 18 (E18d), postnatal day 5 (P5d), postnatal day 19 (P19d), postnatal day 40 (P40d), and postnatal year 1 (P1.. Read More»

Genet. Mol. Res. 14(3):

2015.August.19.22

DOI:

10.4238/2015.August.19.22

Expression pattern of JMJD1C in oocytes and its impact on early embryonic development

Authors: C.H. Li, Y. Gao, S. Wang, F.F. Xu, L.S. Dai, H. Jiang, X.F. Yu, C.Z. Chen, B. Yuan and J.B. Zhang

Cell reprogramming mediated by histone methylation and demethylation is crucial for the activation of the embryonic genome in early embryonic development. In this study, we employed quantitative real-time polymerase chain reaction (qRT-PCR) to detect mRNA levels and expression patterns of all known histone demethylases in early germinal vesicle stage and .. Read More»

Genet. Mol. Res. 14(4):

2015.December.23.12

DOI:

10.4238/2015.December.23.12

Effect of Ginkgo biloba extract on matrix metalloproteinase-3 expression in a rat model of chondrocyte injury

Authors: J.Z. Zeng, L.F. Ma, H. Meng, H.M. Yu, Y.K. Zhang and A. Guo

A rat model with cartilage chondrocyte injury was established using interleukin-1β (IL-1β) to investigate the effect of Ginkgo biloba extract (EGb) on matrix metalloproteinase-3 (MMP-3) expression. Rat chondrocytes were extracted and randomly divided into six groups: control group, IL-1β (model) group, IL-1β + dexamethasone group, and .. Read More»

Genet. Mol. Res. 14(4):

2015.December.23.15

DOI:

10.4238/2015.December.23.15

Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense

Authors: S.B. Jin, H.T. Fu, S.F. Jiang, Y.W. Xiong, H. Qiao, W.Y. Zhang, Y.S. Gong and Y. Wu

The oriental river prawn, Macrobrachium nipponense, is an important aquaculture species in China. The androgenic gland produces hormones that play crucial roles in the differentiation of crustaceans to the male sex. MicroRNA (miRNA) post-transcriptionally regulates many protein-coding genes, influencing important biological and metabolic processes. Howeve.. Read More»

Genet. Mol. Res. 14(4):

2015.December.23.27

DOI:

10.4238/2015.December.23.27

Effect of acute/subchronic samarium exposure on the concentration, motility, and morphology of sperm in male mice

Authors: D.Y. Zhang, X.Y. Shen, X.L. Xu, Q. Ruan, SS. Hu, Y.Y. Chen and Z.M. Wang

Male ICR mice were orally administered samarium nitrate [Sm(NO3)3] to investigate its effects on sperm concentration and sperm quality. After acute exposure to ≥2880.00 mg/kg Sm(NO3)3 via intragastric gavage, sperm motility and acrosome integrity were decreased, and the sperm malformation percentage was increased (P 3)3 adm.. Read More»

Genet. Mol. Res. 15(2):

gmr.15028807

DOI:

10.4238/gmr.15028807

Expressed sequence tag analysis of functional genes associated with adventitious rooting in Liriodendron hybrids

Authors: Y.D. Zhong, X.Y. Sun, E.Y. Liu, Y.Q. Li, Z. Gao and F.X. Yu

Liriodendron hybrids (Liriodendron chinense x L. tulipifera) are important landscaping and afforestation hardwood trees. To date, little genomic research on adventitious rooting has been reported in these hybrids, as well as in the genus Liriodendron. In the present study, we used adventitious roots to construct the first cDNA.. Read More»

Genet. Mol. Res. 15(2):

Influence of sirolimus-induced TGF-�² secretion on mouse Treg cell proliferation

Authors: J.N. Li, J.X. Li, H.L. Huang, X.L. Zhu, H. Ding, C.F. Huang, J. Lin, J.G. Huang, Z.C. Wu, M. Ashraf, Y.G. Wang, X.K. Li, S.Y. Zheng, J.M. Chen, H.M. Guo, J. Zhuang and P. Zhu

We examined the effects of co-culturing CD4+ CD25+ Treg cells with sirolimus or cyclosporin A on Treg cell proliferation and differentiation and on transforming growth factor-β (TGF-β) and Foxp3 expression. CD4+ CD25+ Treg cells were harvested from mononuclear cells of spleens of C57BL/6 mice using immunomagnetic beads and divided into control, .. Read More»

Genet. Mol. Res. 14(4):

2015.December.28.4

DOI:

10.4238/2015.December.28.4

Effect of MSTN propeptide protein on the growth and development of Altay lamb muscle

Authors: W. Du, Y. Zhang, J.Z. Yang, H.B. Li, J. Xia, N. Li, J.S. Zhang, X.M. Yan and Z.Y. Zhou

Prokaryotic expression technology was used to express maltose-binding protein binding myostatin (MSTN) propeptide fusion protein. Six disease-free Altay lambs were used in this study. The right leg gastrocnemii were injected with MSTN recombinant propeptide protein. The left leg gastrocnemii (the control group) were injected w.. Read More»

Genet. Mol. Res. 15(2):

gmr.15027778

DOI:

10.4238/gmr.15027778

Morphological and phylogeographic analysis of Brazilian tortoises (Testudinidae)

Authors: T.L. Silva, L.P.R. Venancio and C.R. Bonini-Domingos

Genet. Mol. Res. 14(4):

2015.December.28.8

DOI:

10.4238/2015.December.28.8