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Animal Genetics

Animal Genetics   Research Article

Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia

Authors: C. Guti�©rrez-Vergara, J. Quintero, J.F. Duarte, J.P. Suesc�ºn and A. L�³pez-Herrera

Hepatitis E is a form of endemic acute hepatitis found in humans in many countries worldwide and is caused by the hepatitis E Virus (HEV). Detection of HEV in pigs indicates that they may be carriers, possibly through zoonosis. The prevalence of HEV in pigs in Colombia is unknown. Studies in the US found that 11% of pig livers.. Read More»

Genet. Mol. Res. 14(1):
2015.March.31.20
DOI:
10.4238/2015.March.31.20
Animal Genetics   Research Article

Association between male infertility and androgen receptor mutations in Brazilian patients

Authors: C.O.A. Melo, A.R. Danin, D.M. Silva, J.A. Tacon, K.K.V.O. Moura, E.O.A. Costa and A.D. da Cruz

The androgen receptor is encoded by a single-copy gene located in the long arm of the X chromosome (Xq11-12); it consists of eight exons and encodes an intracellular transcription factor that belongs to the steroid/nuclear receptor superfamily. Disturbances in the function of the androgen receptor can lead to several forms of .. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr661
DOI:
10.4238/vol9-1gmr661
Animal Genetics   Research Article

Somatic cell nuclear transfer is associated with altered expression of angiogenic factor systems in bovine placentomes at term

Authors: D.B. Campos, P.C. Papa, J.E.B. Marques Jr., F. Garbelotti, L.A. F�¡tima, L.P. Artoni, E.H. Birgel Jr., F.V. Meirelles, J. Buratini Jr., R. Leiser and C. Pfarrer

Low efficiency of somatic cell cloning by nuclear transfer has been associated with alterations of placental vascular architecture. Placental growth and function depend on the growth of blood vessels; VEGF-A and bFGF are the most important factors controlling neovascularization and vascular permeability in the placenta. We hyp.. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr729
DOI:
10.4238/vol9-1gmr729
Animal Genetics   Research Article

Enzyme activity and morphological change in the spleens of crucian carp in the Yongcheng coal mine subsidence area, China

Authors: Y.F. Yan, J.Y. Yang, and J.Y. Lin

The goal of the current study was to investigate the effects of pollution on aquatic organisms in the Yongcheng coal mine subsidence area. Crucian carp (Carassius auratus) were collected from Yongcheng natural fishpond (experimental group) and Tianmu Lake (control group), and the spleens were isolated for analysis. Subsequentl.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027782
DOI:
10.4238/gmr.15027782
Animal Genetics   Research Article

RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas

Authors: J.C.V. Guerra Jr., M.R.C. Issa, F.E. Carneiro, R. Strapazzon and G. Moretto

The mite Varroa destructor is the main pest causing damage to apiculture worldwide. In Brazil and other parts of the world, where bees of African origin and their hybrids predominate, the bees can survive these mites without treatment. Studies have shown a correlation between the various genotypes of the mite and its fertility.. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr696
DOI:
10.4238/vol9-1gmr696
Animal Genetics   Research Article

Transferability of microsatellite loci from exotic Cervidae to Brazilian brocket deer (Mazama spp, Mammalia: Cervidae)

Authors: A.M.B. Mantellatto, E.A.G. Carnelossi and J.M.B. Duarte

Transferability of microsatellite loci between closely related species has been reported in several species. This helps reduce costs involved with the development of primers for newly investigated species. Fifteen microsatellite primers developed for Rangifer tarandus, Cervus elaphus, C. axis, and Moschus berezovskii were test.. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr720
DOI:
10.4238/vol9-1gmr720
Animal Genetics   Research Article

Molecular cloning and expression of epsilon toxin from Clostridium perfringens type D and tests of animal immunization

Authors: A.M. Souza, J.K.P. Reis, R.A. Assis, C.C. Horta, F.F. Siqueira, S. Facchin, E.R. Alvarenga, C.S. Castro, F.M. Salvarani2, R.O.S. Silva, P.S. Pires, C. Contigli, F.C.F. Lobato and E. Kalapothakis

Epsilon toxin produced by Clostridium perfringens types B and D causes enterotoxemia in sheep, goats and calves. Enterotoxemia can cause acute or superacute disease, with sudden death of the affected animal. It provokes huge economic losses when large numbers of livestock are affected. Therapeutic intervention is challenging, .. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr711
DOI:
10.4238/vol9-1gmr711
Animal Genetics   Research Article

Karyotypic evolution trends in Rhamdia quelen (Siluriformes, Heptapteridae) with considerations about the origin and differentiation of its supernumerary chromosomes

Authors: C. Garcia, C. Oliveira and L.F. Almeida-Toledo

Among catfish species of the genus Rhamdia reported for the Brazilian territory, R. quelen is the most widespread, being found in nearly all hydrographic basins of Brazil. Nowadays, R. quelen is a synonym for at least 47 other species in this genus, its taxonomic status still being controversial. The available cytogenetic repo.. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr750
DOI:
10.4238/vol9-1gmr750
Animal Genetics   Research Article

Conserved baculoviral ORFs 10 and 14 from Bombyx mori multiple nucleopolyhedrovirus

Authors: S.A. Santos, J.L.C. Silva, V.A. Balani, F.A.V. Seixas and M.A. Fernandez

ORFs 10 and 14 from Bombyx mori multiple nucleopolyhedrovirus (BmMNPV) were amplified, cloned and sequenced. Nucleotide analysis of these genes and those of other baculoviruses showed that these genes are highly conserved. The p10 protein from BmMNPV ORF10 has 70 amino acid residues similar to that of the four other known BmNP.. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr723
DOI:
10.4238/vol9-1gmr723
Animal Genetics   Research Article

Selection and use of reference genes in mouse mammary glands

Authors: L.Q. Han, G.Y. Yang, H.S. Zhu, Y.Y. Wang, L.F. Wang, Y.J. Guo, W.F. Lu, H.J. Li and Y.L. Wang

Obtaining quantitative data concerning gene expression is important for understanding milk synthesis in mammary glands. Quantitative real-time PCR (qRT-PCR) is an efficient tool to calculate gene expression; however, it is necessary to find valid reference genes for normalization of qRT-PCR data. We applied the geNorm software.. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr724
DOI:
10.4238/vol9-1gmr724