Animal Genetics
Differential gene expression and functional analysis of pit cells from regenerating rat liver
Authors: C.S. Xu, X.G. Chen, C.F. Chang, G.P. Wang, W.B. Wang, L.X. Zhang, Q.S. Zhu and L. Wang
Hepatic pit cells are a population of large granular lymphocytes that substantially contribute to hepatic immunity. Studies have proven that pit cells have a role in liver regeneration, but the details of the relationship between pit cells and liver regeneration is not clear at present. We subjected rats to a two-third hepatectomy; pit cells with high pur.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1052
- DOI:
- 10.4238/vol10-2gmr1052
Polymorphism of somatostatin gene and its association with growth traits in Chinese cattle
Authors: L. Gao, L.S. Zan, H.B. Wang, R.J. Hao and X. Zhong
Somatostatins play a crucial role in the regulation of growth and development in vertebrates, especially muscle growth. We assessed the association of somatostatin gene polymorphisms with growth traits by PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) and DNA sequencing methods in 694 individuals .. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1029
- DOI:
- 10.4238/vol10-2gmr1029
Microsatellite markers derived from Japanese scallop (Mizuhopecten yessoensis) expressed sequence tags
Authors: Y.F. Li, X.G. Gao, W.D. Liu, Y. Tian, L. Gao, S.L. Li and C.B. He
Japanese scallop (Mizuhopecten yessoensis) is a cold-water shellfish, and a species of economic importance in China. In this study, we developed and evaluated simple sequence repeat (SSR) markers from the expressed sequence tags (ESTs) of M. yessoensis. The characteristics of 12 EST-SSR loci were investigated in 30 individual scallops, and the result reve.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.24.3
- DOI:
- 10.4238/2014.March.24.3
Isolation and characterization of novel microsatellite markers in commercial selected golden Malaysian arowana fish, Scleropages formosus (Osteoglossidae)
Authors: B. Manoharan, Z. Sulaimen, F. Omar, R.Y. Othman, S.Z. Mohamed and S. Bhassu
Malaysian arowana (dragonfish; Scleropages formosus) is an ancient osteoglossid fish from southeast Asia. Due to the high demand of the ornamental fish trade and because of habitat loss, the species is close to extinction. We isolated and characterized 10 polymorphic microsatellites of this species, using 5'-anchored PCR. The .. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr944
- DOI:
- 10.4238/vol10-2gmr944
Molecular cloning, characterization and expression analysis of a Doublesex gene from Daphnia carinata (Crustacea: Cladocera) during different reproductive stages
Authors: M.Q. Zhang, C.A. Ma, W.W. Lv, Y.H. Huang, D.L. Wang and Y.L. Zhao
To better understand the reproductive transformation mechanism of Daphnia carinata, a Doublesex (Dsx) gene was cloned based on rapid amplification of cDNA ends (RACE), and was designated DapcaDsx2. Next, we compared similarities and assumed homology based on deduced amino acid sequences. It showed 97.52, 87.94, and 85.11% identity to orthologous genes in .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.10
- DOI:
- 10.4238/2015.June.1.10
Structural analysis of a 4414-bp element in Drosophila melanogaster
Authors: B. Yu, X.T. Wang, H.W. Li, C.J. Zhao, C.X. Wu and X.M. Deng
We cloned a 4414-bp element from a mutant of Drosophila melanogaster. Its insertion site was 18,929,626 bp. Analysis of the nucleotide and amino acid sequences demonstrated that the element is homologous to Pifo_I, first obtained from D. yabuka, which belongs to the gypsy/Ty3 subfamily. We also obtained a 3754-bp length elemen.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr987
- DOI:
- 10.4238/vol10-2gmr987
Comparative transcriptome analysis reveals three potential antiviral signaling pathways in lymph organ tissue of the red swamp crayfish, Procambarus clarkii
Authors: Z.-Q. Du
The red swamp crayfish (Procambarus clarkii) is one of the most economically important farmed aquatic species in China. Compared with its relatively well-known antibacterial and antifungal mechanisms, the antiviral mechanism is still unclear. We used Illumina-based RNA sequencing and bioinformatic technology to obtain high-qua.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048858
- DOI:
- 10.4238/gmr15048858
Proteomic analysis revealed the altered kidney protein profile of a Cyld knockout mouse model
Authors: Y. Zhao, Y. Zhang, H.B. Song, F. Wu, X.L. Wang, S.-C. Sun, T.X. Cui and D.Q. Tang
The aim of this study was to compare the proteomics pattern of the kidneys from Cyld knockout mice with that from normal mouse kidneys and establish a preliminary understanding of the role of Cyld in the kidney. Proteins from the kidneys of knockout Cyld mice and wild-type mice were extracted, isobaric tags for relative and absolute quantitation (iTRAQ) w.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.14
- DOI:
- 10.4238/2015.June.1.14
Genetic variability in three populations of Hypostomus hermanni (Teleostei: Loricariidae) found in the basins of IvaÃ? River, TietÃ?ª River, and SapucaÃ?Â-Mirim River of Brazil
Authors: R.S. Vieira and E. Renesto
The genetic variability in three populations of Hypostomus hermanni from the tributaries of the rivers Ivaí (Keller), Tietê (Piracicaba), and Sapucaí (Sapucaí-Mirim) was determined using electrophoresis on starch gel. The variability in the genes for eight enzymes, namely, aspartate aminotransferase .. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049056
- DOI:
- 10.4238/gmr15049056
Analysis of intervertebral disc-related genes
Authors: D.J. Wu, K. Chen, X.Z. Wei, H.J. Ni, S.Z. Yu, X.D. Zhu and M. Li
Intervertebral disk disease is a common clinical disorder manifested by pain, ataxia, paresis, motor paralysis, and sensorimotor paralysis. The clinical features, diagnosis, and treatment of cervical and thoracolumbar disk disease have been unclear until now. In this study, some differentially expressed genes were identified, .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.24.7
- DOI:
- 10.4238/2014.March.24.7