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Animal Genetics

Animal Genetics   Research Article

Evaluation of microsporogenesis in an interspecific Brachiaria hybrid (Poaceae) collected in distinct years

Authors: V.A. Fuzinatto, M.S. Pagliarini and C.B. Valle

Microsporogenesis in an interspecific Brachiaria hybrid, grown in the field under natural environmental conditions in Brazilian savannas, was analyzed in three distinct years of collection. Several types of meiotic abnormalities were recorded during those three years, but varied in type and frequency depending on the year. The.. Read More»

Genet. Mol. Res. 7(2):
Animal Genetics   Research Article

Molecular cloning, characterization and association analysis of the promoter region of the bovine CDK6 gene

Authors: Y.F. Liu, L.S. Zan, W.T. Cui, Y.P. Xin, Y. Jiao and K. Li

Cyclin-dependent kinase 6 (CDK6) is a key element of D-type cyclin holoenzymes. It is involved in the regulation of the G1-phase of the cell cycle and is considered to be an important candidate gene for selection of body measurement traits through marker-assisted selection. We cloned the promoter sequence of this gene in bovin.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1466
DOI:
10.4238/vol10-3gmr1466
Animal Genetics   Research Article

Involvement of the mitfa gene in the development of pigment cell in Japanese ornamental (Koi) carp (Cyprinus carpio L.)

Authors: J.H. Liu, S. Wen, C. Luo, Y.Q. Zhang, M. Tao, D.W. Wang,S.M. Deng and Y.M. Xiao

A colored phenotype is an important feature of ornamental fish. In mammals, microphthalmia-associated transcription factor (MITF) was found to regulate the development of melanocytes. In this study, the mitfa cDNA was first cloned from the Japanese ornamental (Koi) carp (Cyprinus carpio L.), an important ornamental freshwater fish. The full-length cDNA of.. Read More»

Genet. Mol. Res. 14(1):
http://dx.doi.org/2015.March.31.7
DOI:
http://dx.doi.org/10.4238/2015.March.31.7
Animal Genetics   Research Article

Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: the experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH

Authors: C.B. Vendrame-Goloni, A.B. Carvalho-Salles, M.A. Ruiz,O. Ricci J�ºnior, M. Varella-Garcia4 and A.C. Fett-Conte

Investigation of the efficacy of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in chronic myeloid leukemia patients is essential to predict prognosis and survival. In 20 patients treated at the Bone Marrow Transplantation Unit of São José do Rio Preto (São Paulo, Brazil), we used fluoresce.. Read More»

Genet. Mol. Res. 7(2):
vol7-2gmr396
DOI:
10.4238/vol7-2gmr396
Animal Genetics   Research Article

Molecular characteristics and expression of calmodulin cDNA from the freshwater pearl mussel, Hyriopsis schlegelii.

Authors: L.-G. Zeng, J.-H. Wang, Y.-J. Li, J.-Q. Sheng, Q. Gu and Y.-J. Hong

Calmodulin (CaM) is a multifunctional intracellular calcium ion receptor protein that participates in a range of cellular processes, including calcium metabolism in mussels. To investigate the role of CaM in freshwater mollusk shell calcium metabolism, the full-length CaM cDNA was isolated from the freshwater pearl mussel, Hyr.. Read More»

Genet. Mol. Res. 11(1):
http://dx.doi.org/2012.January.9.5
DOI:
http://dx.doi.org/10.4238/2012.January.9.5
Animal Genetics   Research Article

Primed in situ labeling for detecting single-copy genes

Authors: J.-L. Gao, Y. Nie and X.-P. Ding

In order to analyze male sterility caused by deletion of SRY and DAZ, we examined the accuracy and cost-effectiveness of a modified primed in situ labeling (PRINS) technique for detection of single-copy genes. Peripheral blood samples were collected from 50 healthy men; medium-term cultured lymphocytes from these samples were .. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1391
DOI:
10.4238/vol10-3gmr1391
Animal Genetics   Research Article

Cloning and sequence analysis of sucrose phosphate synthase gene from varieties of Pennisetum species

Authors: H.C. Li, H.B. Lu, F.Y. Yang, S.J. Liu, C.J. Bai and Y.W. Zhang

Sucrose phosphate synthase (SPS) is an enzyme used by higher plants for sucrose synthesis. In this study, three primer sets were designed on the basis of known SPS sequences from maize (GenBank: NM_001112224.1) and sugarcane (GenBank: JN584485.1), and five novel SPS genes were identified by RT-PCR from the genomes of Pennisetum spp (the hybrid P. american.. Read More»

Genet. Mol. Res. 14(1):
http://dx.doi.org/2015.March.31.10
DOI:
http://dx.doi.org/10.4238/2015.March.31.10
Animal Genetics   Research Article

Relationship between the magnitude of the inbreeding coefficient and milk traits in Holstein and Jersey dairy bull semen used in Brazil

Authors: M.P. Soares, L.G. Gaya, L.H. Lorentz, F. Batistel, G.A. Rovadoscki, E. Ticiani, V. Zabot, Q. Di Domenico, A.P. Madureira and S.F.N. P�©rtile

Artificial insemination has been used to improve production in Brazilian dairy cattle; however, this can lead to problems due to increased inbreeding. To evaluate the effect of the magnitude of inbreeding coefficients on predicted transmitting abilities (PTAs) for milk traits of Holstein and Jersey breeds, data on 392 Holstein.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1010
DOI:
10.4238/vol10-3gmr1010
Animal Genetics   Research Article

Molecular characterization, polymorphism of the ACOX1 gene and association with ultrasound traits in Bos taurus

Authors: Y. Jiao, L.S. Zan, Y.F. Liu and H.B. Wang

Acyl-coenzyme A oxidase 1 (ACOX1) is the first enzyme in peroxisomal fatty acid β-oxidation; it is rate-limiting and plays a key role in fatty acid metabolism and fat deposition. ACOX1 is an important candidate gene for meat quality selection through marker-assisted selection. Genomic structural analysis showed that bovin.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1036
DOI:
10.4238/vol10-3gmr1036
Animal Genetics   Research Article

cDNA cloning and mRNA expression of a tandem-repeat galectin (PoGal2) from the pearl oyster, Pinctada fucata

Authors: D.C. Zhang, Y.T. Hu, H.Y. Guo, S.G. Cui, T.F. Su1 and S.G. Jiang

Galectins can recognize and specifically bind to β-galactoside residues, playing crucial roles in innate immune responses of vertebrates and invertebrates. We cloned the cDNA of a tandem-repeat galectin from the pearl oyster Pinctada fucata (designated as PoGal2). PoGal2 cDNA is 1347 bp long and consists of a 5êÂ?Â?-untr.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1149
DOI:
10.4238/vol10-3gmr1149