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Sugarcane

Plant Genetics   Research Article

DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA

Authors: S. Tabasum, F.A. Khan, S. Nawaz, M.Z. Iqbal and A. Saeed

DNA profiles of 40 sugarcane genotypes were constructed with 30 RAPD markers. Sugarcane genotypes of both Saccharum officinarum and S. barberi were included in this study. Multiple alleles were detected from each RAPD; there was a high level of polymorphism. On average, 7.93 alleles were produced per primer, giving a total of .. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr709
DOI:
10.4238/vol9-1gmr709
Microbial Genetics   Research Article

Molecular characterization of endophytes isolated from Saccharum spp based on esterase and ribosomal DNA (ITS1-5.8S-ITS2) analyses

Authors: A.C. Leme, M.R.R. Bevilaqua, S.A. Rhoden, C.A. Mangolin, M.F.P.S. Machado and J.A. Pamphile

This study used esterases and ribosomal DNA (rDNA) markers to determine endophytic variability in order to better understand endophyte-host interactions. Polyacrylamide gel electrophoresis and esterase isoenzymes (EST; EC 3.1.1.3), with a-naphthyl acetate and β-naphthyl acetate as substrates, were used to assess relationships among endophytes. ITS1-5.. Read More»

Genet. Mol. Res. 12(3):
2013.September.27.11
DOI:
10.4238/2013.September.27.11
Plant Genetics   Research Article

Identification of smut-responsive genes in sugarcane using cDNA-SRAP

Authors: N. Huang, Y.Y. Zhang, X.H. Xiao, L. Huang, Q.B. Wu, Y.X. Que and L.P. Xu

Sugarcane smut, caused by the fungus Sporisorium scitamineum, is one of the main diseases that affect sugarcane worldwide. In the present study, the cDNA-SRAP technique was used to identify genes that are likely to be involved in the response of sugarcane to S. scitamineum infection. In total, 21 bands with significant differential expression during cDNA-.. Read More»

Genet. Mol. Res. 14(2):
2015.June.18.23
DOI:
10.4238/2015.June.18.23
Plant Genetics   Research Article

Genetic diversity among Puccinia melanocephala isolates from Brazil assessed using simple sequence repeat markers

Authors: R.F. Peixoto-Junior, S. Creste, M.G.A. Landell, D.S. Nunes, A. Sanguino, M.F. Campos, R. Vencovsky, E.V. Tambarussi and A. Figueira

Brown rust (causal agent Puccinia melanocephala) is an important sugarcane disease that is responsible for large losses in yield worldwide. Despite its importance, little is known regarding the genetic diversity of this pathogen in the main Brazilian sugarcane cultivation areas. In this study, we characterized the gen.. Read More»

Genet. Mol. Res. 13(3):
2014.September.26.23
DOI:
10.4238/2014.September.26.23