Sugarcane
DNA methylation in sugarcane somaclonal variants assessed through methylation-sensitive amplified polymorphism
Authors: J.H.M.B. Francischini, E.L. Kemper, J.B. Costa, J.R.V. Manechini and L.R. Pinto
Micropropagation is an important tool for large-scale multiplication of plant superior genotypes. However, somaclonal variation is one of the drawbacks of this process. Changes in DNA methylation have been widely reported as one of the main causes of somaclonal variations in plants. In order to investigate the occurrence of ch.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029585
- DOI:
- 10.4238/gmr16029585
Selection between and within full-sib sugarcane families using the modified BLUPIS method (BLUPISM)
Authors: R.D. Castro1, L.A. Peternelli1, M.D.V. Resende2, C.D. Marinho1, P.M.A. Costa1, M.H.P. Barbosa3 and E.F.A. Moreira1
The objective of this study was to assess the efficiency of a modification of the simulated individual best linear unbiased prediction (BLUPIS) procedure, which is used for the approximation of classic individuals (BLUPI) for selection between and within sugarcane families. A total of 110 full-sib families were employed in an experiment initiated in 2007 .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017334
- DOI:
- 10.4238/gmr.15017334
Microbial diversity in the larval gut of field and laboratory populations of the sugarcane weevil Sphenophorus levis (Coleoptera, Curculionidae)
Authors: R. Rinke, A.S. Costa, F.P.P. Fonseca, L.C. Almeida, I. Delalibera J�ºnior and F. Henrique-Silva
The sugarcane weevil, Sphenophorus levis, is a wide-spread sugarcane pest in Brazil. Sphenophorus levis may depend on microorganisms that inhabit its intestinal tract. We examined the diversity of the gut microbiota of S. levis, which was characterized using culture-dependent and culture-independent methods. Analysis of 16S rR.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.1.1
- DOI:
- 10.4238/2011.November.1.1
Phylogenetic relationships among Saccharum clones in Pakistan revealed by RAPD markers
Authors: S. Nawaz, F.A. Khan, S. Tabasum, M. Zakria, A. Saeed and M.Z. Iqbal
Forty sugarcane genotypes (clones), including elite lines, commercial cultivars of Saccharum officinarum and S. barberi clones, were fingerprinted with 30 RAPD markers, using a PCR-based marker assay. The genetic distance for RAPD data was determined according to Nei, and relationships between accessions were graphed in a dend.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr903
- DOI:
- 10.4238/vol9-3gmr903
Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers
Authors: Y.Y. Zhang, N. Huang, X.H. Xiao, L. Huang, F. Liu, W.H. Su and Y.X. Que
Sugarcane smut caused by the fungus Sporisorium scitamineum is a worldwide disease and also one of the most prevalent diseases in sugarcane production in mainland China. To study molecular variation in S. scitamineum, 23 S. scitamineum isolates from the 6 primary sugarcane production areas in mainland, China (Guangxi, Yunnan, Guangdong, Hainan, Fujian, an.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.15
- DOI:
- 10.4238/2015.July.14.15
Variability of red rot-resistant somaclones of sugarcane genotype S97US297 assessed by RAPD and SSR
Authors: M.T.H. Shahid, F.A. Khan, A. Saeed and I. Fareed
Sugarcane breeding under climatic conditions of Pakistan is very difficult due to unavailability of viable fuzz (seed). Somaclonal variation can provide an alternative for improvement of existing genotypes. Six hundred and twenty-seven somaclones were developed from sugarcane genotype S97US297, and protocols for callogenesis a.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1122
- DOI:
- 10.4238/vol10-3gmr1122
Establishment of an in vitro regeneration system for genetic transformation of selected sugarcane genotypes
Authors: S. Ijaz, I.A. Rana, I.A. Khan and M. Saleem
A good culture system provides considerable quantities of highly regenerable target tissues. Embryogenic callus cultures are ideal for micro-projectile-mediated transformation, because regenerable cells are not very stable. Effective exploitation of genetic transformation requires good regeneration systems. We selected three s.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.6.4
- DOI:
- 10.4238/2012.March.6.4
Paternity identification in sugarcane polycrosses by using microsatellite markers
Authors: M.A. Xavier , L.R. Pinto , T.M. F�¡vero, D. Perecin , L.A. Carlini-Garcia and M.G.A. Landell
Although polycrosses have been used to test the potential of cross-combination of a large number of sugarcane parents, the male parent of the half-sib progenies produced is unknown. The present study aimed to integrate the molecular marker technology to the sugarcane polycross approach by the application of microsatellite mark.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.31.7
- DOI:
- 10.4238/2014.March.31.7
Genetic divergence and admixture of ancestral genome groups in the sugarcane variety �RB867515� (Saccharum spp
Authors: G.B. Maranho, R.C. Maranho, R. Desordi, A.F. das Neves, C.A. Mangolin and M.F.P.S. Machado
We analyzed 80 plants of the sugarcane (Saccharum spp) variety ‘RB867515’ in order to investigate its diversity and genetic structure at the molecular level. Four simple sequence repeat (SSR) loci (UGSM51, SMC1237, SEGMS1069, and UGSM38) and five expressed sequence tag (EST)-SSR loci (ESTA68, ESTB92, ESTB145, ESTC6.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049209
- DOI:
- 10.4238/gmr15049209
Diversity of endophytic bacteria in Brazilian sugarcane
Authors: G.S. Magnani, C.M. Didonet, L.M. Cruz, C.F. Picheth, F.O. Pedrosa and E.M. Souza
Endophytic bacteria live inside plant tissues without causing disease. Studies of endophytes in sugarcane have focused on the isolation of diazotrophic bacteria. We examined the diversity of endophytic bacteria in the internal tissues of sugarcane stems and leaves, using molecular and biochemical methods. Potato-agar medium wa.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr703
- DOI:
- 10.4238/vol9-1gmr703