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Microbial Genetics   Research Article

Differential expression of genes associated with low-level vancomycin resistance in Staphylococcus aureus

Authors: Auttawit Sirichoat, Viraphong Lulitanond, Sujintana Wongthong, Aroonwadee Chanawong, Ratree Tavichakorntrakool and Aroonlug Lulitanond

Heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) and vancomycin-intermediate S. aureus (VISA) have been associated with treatment failure, and hVISA cannot be detected by routine disc diffusion test. Currently, there is no specific genetic marker for detection of these strains. The aim of the current study was to inves.. Read More»

Genet. Mol. Res. 18(2):
Microbial Genetics   Research Article

Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis

Authors: A.R.E.O. Xavier, A.C. Almeida, C.N. Souza, L.M.V Silva, A.X.A. Ruas, D.A. Sanglard, A.F.M. JĂșnior , A.M.E. Oliveira and M.A.S. Xavier

The Staphylococcus aureus is the most common isolated microorganism in ruminant animal species diagnostic with clinical or subclinical mastitis. Dairy herds with these diseases can transfer S. aureus into the milk supply, which can lead to food poisoning in humans. The objective of this study was to evaluate the profile of ant.. Read More»

Genet. Mol. Res. 16(2):
Animal Genetics   Research Article

Methylation analysis of CXCR1 in mammary gland tissue of cows with mastitis induced by Staphylococcus aureus

Authors: Y.J. Mao, X.R. Zhu, R. Li, D. Chen, S.Y. Xin, Y.H. Zhu, X.X. Liao, X.L. Wang, H.M. Zhang, Z.P. Yang and L.G. Yang

Mastitis is the most important disease in the global dairy industry, and causes large economic losses. Staphylococcus aureus is one of most common pathogens that cause bovine mastitis. CXCR1 has been implicated as a prospective genetic marker for mastitis resistance in dairy cows; CXCR1 expression significantly increases when .. Read More»

Genet. Mol. Res. 14(4):
Microbial Genetics   Research Article

Molecular analysis and frequency of Staphylococcus aureus virulence genes isolated from bloodstream infections in a teaching hospital in Tianjin, China

Authors: L.X. Wang Z.D. Hu Y.M. Hu B. Tian Jing Li F.X. Wang H. Yang H.R. Xu Y.C. Li J. Li

Staphylococcus aureus is an important cause of bloodstream infections worldwide. We examined the prevalence of genes that encode erythromycin ribosome methylase and bacterial toxins in S. aureus collected from bloodstream infections. Sixty different S. aureus isolates were obtained from blood cultures of patients who were admitted to a Teaching Hospital i.. Read More»

Genet. Mol. Res. 12(1):
Animal Genetics   Research Article

Evaluation of methods of DNA extraction from Staphylococcus aureus in milk for use in real-time PCR

Authors: A.G. Dibbern, B.G. Botaro, M.P. Viziack, L.F.P. Silva and M.V. Santos

The aim of this study was to evaluate the repeatability and performance of 4 methods of extracting DNA from Staphylococcus aureus (SAU) and the gene encoding bovine mitochondrial cytochrome B (BMCB) in milk samples from cows with subclinical mastitis for use in amplification by real-time polymerase chain reaction. Two milk samples were obtained from cows .. Read More»

Genet. Mol. Res. 14(1):
Animal Genetics   Research Article

An experimental model for Staphylococcus aureus hepatic abscess in Bama minipig

Authors: R.G. Zhang, X.D. Wang*, X.L. Zhang and Y.S. Yang

Pyogenic hepatic abscess (PHA) is a rare but potentially serious disease. Investigations of new therapeutic methods urgently need experimental support in corresponding animal models. However, to date, few studies have evaluated PHA in the minipig. The linear regression equation of the Staphylococcus aureus ATCC 25923 .. Read More»

Genet. Mol. Res. 13(3):
Microbial Genetics   Research Article

Distribution of food-borne Staphylococcus aureus enterotoxin genes

Authors: W.D. Hu

We identified and analyzed 5 new-type enterotoxin genes, including SEj, SEl, SEq, SEm, and SEr, to explore the distribution of 5 enterotoxin genes in Staphylococcus aureus of different origins as well as their correlations and differences. We examined the distribution of the S. aureus enterotoxin genes and their pathogenic mechanisms. A total of 660 speci.. Read More»

Genet. Mol. Res. 15(1):
Animal Genetics   Research Article

Evaluation of the humoral immune response in BALB/c mice immunized with a naked DNA vaccine anti-methicillin-resistant Staphylococcus aureus

Authors: D.M. Roth, J.P.M. Senna and D.C. Machado

Methicillin-resistant Staphylococcus aureus (MRSA) is the major pathogen involved in nosocomial infections, leading to high rates of morbidity and mortality in hospitals worldwide. The methicillin resistance occurs due to the presence of an additional penicillin-binding protein, PBP2a, which has low affinity for β-lactam .. Read More»

Genet. Mol. Res. 5(3):
Plant Genetics   Research Article

Epidemiological investigation of community-acquired Staphylococcus aureus infection

Authors: W. Jiang, Z. Zhou, K. Zhang and Y. Yu

The current study aimed to reveal the pathogenic and spreading mechanisms of community-acquired Staphylococcus aureus (CA-SA) by analyzing its prevalence, drug resistance, virulence, and pathogenic factors. Historical information regarding CA skin and soft tissue infections were collected and disease characteristics were analyzed. Isolated CA-SA strains w.. Read More»

Genet. Mol. Res. 12(4):
Human Genetics   Research Article

Rifampicin resistance among multi-resistant MRSA clinical isolates from Chennai, India, and their molecular characterization

Authors: K. Murugan, K. Kavitha and S. Al-Sohaibani

High-level methicillin-resistant Staphylococcus aureus (MRSA) isolates show rapid evolution of rifampicin resistance, forcing reliance upon expensive and often inferior antibiotics to manage these infections. Accordingly, this study was conducted to: 1) evaluate the level of multidrug resistance among hospital-associated MRSA isolates from Chennai, India;.. Read More»

Genet. Mol. Res. 14(1):