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Soybean

Human Genetics   Research Article

Positive selection sites in tertiary structure of Leguminosae Chalcone isomerase 1

Authors: R.K. Wang, S.F. Zhan, T.J. Zhao, X.L. Zhou and C.E. Wang

Isoflavonoids and the related synthesis enzyme, chalcone isomerase 1 (CHI1), are unique in the Leguminosae, with diverse biological functions. Among the Leguminosae, the soybean is an important oil, protein crop, and model plant. In this study, we aimed to detect the generation pattern of Leguminosae CHI1. Genome-wide sequence analysis of CHI in 3 Legumin.. Read More»

Genet. Mol. Res. 14(1):
2015.March.20.5
DOI:
10.4238/2015.March.20.5
Plant Genetics   Research Article

Problems with and a system to eliminate single-primer PCR product contamination in simple sequence repeat molecular marker-assisted selection in soybean

Authors: J. Ma, S.C. Guan, D. Yao, Y.F. Wei and P.W. Wang

Polymerase chain reaction (PCR) provides a foundation for simple sequence repeat molecular marker-assisted selection (SSR MAS) in soybean. This PCR system and its various conditions have been optimized by many researchers. However, current research on the optimization of the PCR system focuses on double-primer PCR products. We.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1366
DOI:
10.4238/vol10-3gmr1366
Plant Genetics   Research Article

Genetic diversity and the conservation priority of Glycine soja populations from Northern China

Authors: Y.L. Niu, W.Y. Guo, L.R. Bai and J.C. Zhao

ABSTRACT. Knowledge of the spatial patterns of genetic variation in wild populations has significant implications for in situ conservation and the determination of conservation order. To study the levels of genetic diversity, spatial genetic structures, and genetic distances in Glycine soja, 11 natural populations in northern China were analyzed by estima.. Read More»

Genet. Mol. Res. 14(4):
2015
DOI:
10.4238/2015
Plant Genetics   Research Article

Soybeans as bioreactors for biopharmaceuticals and industrial proteins

Authors: G.R. Vianna, N.B. Cunha, A.M. Murad and E.L. Rech

Plants present various advantages for the production of biomolecules, including low risk of contamination with prions, viruses and other pathogens, scalability, low production costs, and available agronomical systems. Plants are also versatile vehicles for the production of recombinant molecules because they allow protein expr.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1476
DOI:
10.4238/vol10-3gmr1476
Plant Genetics   Research Article

Overexpression of the activated form of the AtAREB1 gene (AtAREB1Ã?Â?QT) improves soybean responses to water deficit

Authors: J.P. Leite, E.G.G. Barbosa, S.R.R. Marin, J.P. Marinho, J.F.C. Carvalho, R.F. Pagliarini, A.S. Cruz, M.C.N. Oliveira, J.R.B. Farias, N. Neumaier, F.C.M. Guimar�£es, T. Yoshida, N. Kanamori, Y. Fujita, K. Nakashima, K.Y. Shinozaki, J.A. Desid�©rio and A.L. Nepomuceno

Abscisic acid-responsive element binding protein (AREB1) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element motif in the promoter region of ABA-inducible genes. Because AREB1 is not sufficient to direct the expression of downstream genes under non-stress conditions, a.. Read More»

Genet. Mol. Res. 13(3):
2014.August.15.10
DOI:
10.4238/2014.August.15.10
Plant Genetics   Research Article

Analysis of quantitative trait loci for main plant traits in soybean

Authors: D. Yao, Z.Z. Liu, J. Zhang, S.Y. Liu, J. Qu, S.Y. Guan, L.D. Pan,D. Wang, J.W. Liu and P.W. Wang

Plant traits are important indices for regulating and controlling yield ability in soybean varieties. It is important to comprehensively study the quantitative trait locus (QTL) mapping for soybean plant traits, cloning related genes, and marker assistant breeding. In this study, 236 F2 generation plants and a derivative group were constructed by using J.. Read More»

Genet. Mol. Res. 14(2):
2015.June.8.8
DOI:
10.4238/2015.June.8.8
Cytogenomics   Research Article

Genomic selection of seed weight based on low-density SCAR markers in soybean

Authors: Y.J. Shu, D.S. Yu, D. Wang, X. Bai, Y.M. Zhu and C.H. Guo

With the development of molecular marker technology, crop breeding has been accelerated by marker-assisted selection for the improvement of quantitative traits. However, due to the traits’ polygenic nature, traditional marker-assisted selection methods are ill-suited for identification of quantitative trait loci. Genomic selection (GS) was introduce.. Read More»

Genet. Mol. Res. 12(3):
2013.July.3.2
DOI:
10.4238/2013.July.3.2
Cytogenomics   Research Article

Gene expression in the lignin biosynthesis pathway during soybean seed development

Authors: A. Baldoni, E.V.R. Von Pinho, J.S. Fernandes, V.M. Abreu and M.L.M. Carvalho

The study of gene expression in plants is fundamental, and understanding the molecular mechanisms involved in important biological processes, such as biochemical pathways or signaling that are used or manipulated in improvement programs, are key for the production of high-quality soybean seeds. Reports related to gene expression of lignin in seeds are sca.. Read More»

Genet. Mol. Res. 12(3):
2013.February.28.2
DOI:
10.4238/2013.February.28.2
Medical Genetics   Research Article

Comparative analysis of soybean genotype resistance to Heterodera glycines and Meloidogyne species via resistance gene analogs

Authors: P.M.H. Vieira, F.A.S. Ar�ªdes, A. Ferreira and M.F.S. Ferreira

Nematodes are important pests of soybean throughout the world and cause high yield losses. As a control strategy, the identification of resistance genes is an important aim of breeding studies. Plants possess resistance genes (R), which are responsible for the recognition of pathogens and activation of the defense system. R ge.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038562
DOI:
10.4238/gmr.15038562
Microbial Genetics   Research Article

Agrobacterium-mediated transformation of the �²-subunit gene in 7S globulin protein in soybean using RNAi technology

Authors: J. Qu, S.Y. Liu, P.W. Wang, S.Y. Guan, Y.G. Fan, D. Yao, L. Zhang and J.L. Dai

The objective of this study was to use RNA interference (RNAi) to improve protein quality and decrease anti-nutritional effects in soybean. Agrobacterium tumefaciens-mediated transformation was conducted using RNAi and an expression vector containing the 7S globulin β-subunit gene. The BAR gene was used as the selective m.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027561
DOI:
10.4238/gmr.15027561