Authors: J. Sun, J. Li, M. Liu, B.B. Zhang, D.M. Li, M. Wang, C. Zhang,W.B. Li, A.Y. Su and X.X. Wu
The development of a genetic transformation system is needed to address the problem of the low efficiency associated with soybean regeneration. To contribute to the enhancement of the soybean regenerative capacity, we explored the developmental mechanisms of soybean regeneration at the molecular level using a suppression subtractive hybridization cDNA librar.. Read More»
Authors: H. Ma, H. Li, J. Li, X.F. Wang, P.C. Wei, L. Li and J.B. Yang
The aim of this study was to develop an event-specific qualitative and real-time quantitative polymerase chain reaction (PCR) method for detection of herbicide-tolerance genetically modified (GM) soybean A2704-12. The event-specific PCR primers were designed, based on the 5'-flanking integration sequence in the soybean genome,.. Read More»
Authors: X.H. Li, B. Wu, Y.B. Kong and C.Y. Zhang
Phosphorus plays a pivotal role in plant growth and development. In this study, we isolated and characterized GmPTF1, a basic helix-loop-helix (bHLH) transcription factor (TF) gene from soybean (Glycine max) with tolerance to inorganic phosphate (Pi) starvation. Alignment analysis indicated that GmPTF1 and other reported bHLH .. Read More»
Authors: L.A. Hegeto, L. Ronqui, A.S. Lapenta and F.A. Albuquerque
The brown stink bug Euschistus heros is the most abundant species of the soybean-sucking bugs, and causes large economic losses. Applying different chemical groups of organosynthetic insecticides for its control increases the potential for resistance. Esterases are a group of enzymes that play a variety of roles in insects, and some of them are related to.. Read More»
Authors: L. Zhang, H.K. Zhao, Y.M. Wang, C.P. Yuan, Y.Y. Zhang, H.Y. Li, X.F. Yan, Q.Y. Li and Y.S. Dong
Cysteine-rich polycomb-like protein (CPP-like) genes are a group of transcription factors with highly conserved cysteine-rich domains and are widely distributed in animals and plants, but do not present in yeast. Previous studies have shown that members of this family play important roles in the development of reproductive tissue and in the control of cel.. Read More»
Authors: S. Meri�§, ��. ��ak�±r, N. Turgut-Kara and ��. Ar�±1,
Despite the controversy about genetically modified (GM) plants, they are still incrementally cultivated. In recent years, many food and feed products produced by genetic engineering technology have appeared on store shelves. Controlling the production and legal presentation of GM crops are very important for the environment an.. Read More»
Authors: S.S. Pereira, F.C.M. Guimar�£es, J.F.C. Carvalho, R. Stolf-Moreira, M.C.N. Oliveira, A.A.P. Rolla, J.R.B. Farias, N. Neumaier and A.L. Nepomuceno
To gain insight into stress-responsive gene regulation in soybean plants, we identified consensus sequences that could categorize the transcription factors MYBJ7, BZIP50, C2H2, and NAC2 as members of the gene families myb, bzip, c2h2, and nac, respectively. We also investigated the evolutionary relationship of these transcript.. Read More»
Authors: X.Y. Cui, Z.Y. Chen, L. Wu, X.Q. Liu, Y.Y. Dong, F.W. Wang and H.Y. Li
The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco pl.. Read More»
Authors: Y.H. Wang, X.J. Zhang and S.J. Fan
Annual wild soybean (Glycine soja Sieb. et Zucc.), the ancestor of cultivated soybean (G. max), is believed to be a potential gene source for further improvement of soybean to cope with environmental stress. In this study, 10 simple sequence repeat (SSR) markers were used to evaluate the genetic diversity and population genetic structure in five wild soyb.. Read More»
Authors: Abud S, de Souza PIM, Vianna GR, Leonardecz E, Moreira CT, Faleiro FG, Junior JN, Monteiro PMFO, Rech EL and Aragao FJL
Evaluation of transgenic crops under field conditions is a fundamental step for the production of genetically engineered varieties. In order to determine if there is pollen dispersal from transgenic to nontransgenic soybean plants, a field release experiment was conducted in the Cerrado region of Brazil. Nontransgenic plants w.. Read More»