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Plant Genetics   Research Article

Minimum number of measurements for evaluating Bertholletia excelsa

Authors: A.B. Baldoni, H. Tonini, F.D. Tardin, S.C.C. Botelho and P.E. Teodoro

Repeatability studies on fruit species are of great importance to identify the minimum number of measurements necessary to accurately select superior genotypes. This study aimed to identify the most efficient method to estimate the repeatability coefficient (r) and predict the minimum number of measurements needed for a more a.. Read More»

Genet. Mol. Res. 16(3):
gmr16039783
DOI:
10.4238/gmr16039783
Plant Genetics   Research Article

Genetic control and transgressive segregation of zinc, iron, potassium, phosphorus, calcium, and sodium accumulation in cowpea (Vigna unguiculata) seeds

Authors: C.A. Fernandes Santos1 and L.S. Boiteux2

Cowpea crop, through combining a range of essential minerals with high quality proteins, plays an important role in providing nutritional security to human population living in semi-arid regions. Studies on genetics of biofortification with essential minerals are still quite scarce, and the major objective of the present study was to provide genetic informat.. Read More»

Genet. Mol. Res. 14(1):
2015.January.16.10
DOI:
10.4238/2015.January.16.10
Plant Genetics   Research Article

Mendelian inheritance, linkage, and genotypic disequilibrium in microsatellite loci of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae-Caesalpinioideae)

Authors: M.A. Moraes, T.Y.K. Kubota, E.C.B. Silva, A.M. Silva, J. Cambuim, M.L.T. Moraes, E. Furlani Junior and A.M. Sebbenn

Hymenaea stigonocarpa is a deciduous and monoecious Neotropical tree species pollinated by bats. Due to overexploitation and habitat destruction, the population size has drastically diminished in nature. No previous study has investigated Mendelian inheritance, linkage, and genotypic disequilibrium in the available microsatellite markers in this species. .. Read More»

Genet. Mol. Res. 15(3):
gmr.15038629
DOI:
10.4238/gmr.15038629
Plant Genetics   Original Articles

A modified method for high-quality DNA extraction for molecular analysis in cereal plants

Authors: Z.H. Pervaiz, N.A. Turi, I. Khaliq, M.A. Rabbani and S.A. Malik

Cereal crops that have rigid non-cellulose components in the cell wall tissues of leaves and high starch and protein content in grains face limitations in DNA extraction. Advanced molecular genetic techniques such as mapping and marker-assisted selection programs require pure and quick DNA extraction. In this study, we developed methods for isolating high.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1346
DOI:
10.4238/vol10-3gmr1346
Cytogenomics   Research Article

Gene expression in the lignin biosynthesis pathway during soybean seed development

Authors: A. Baldoni, E.V.R. Von Pinho, J.S. Fernandes, V.M. Abreu and M.L.M. Carvalho

The study of gene expression in plants is fundamental, and understanding the molecular mechanisms involved in important biological processes, such as biochemical pathways or signaling that are used or manipulated in improvement programs, are key for the production of high-quality soybean seeds. Reports related to gene expression of lignin in seeds are sca.. Read More»

Genet. Mol. Res. 12(3):
2013.February.28.2
DOI:
10.4238/2013.February.28.2
Plant Genetics   Research Article

An optimized preparation method to obtain high-quality RNA from dry sunflower seeds

Authors: X.B. Ma and J. Yang

In an attempt to isolate high-quality, intact total RNA from sunflower (Helianthus annuus) seeds for investigation of the molecular mechanisms of mutations, we tested various procedures, using kits, including RNAiso Plus, RNAiso Plus+RNAiso-mate for Plant Tissue, Trizol, and the Qi method, but no high-quality total RNA of high.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr979
DOI:
10.4238/vol10-1gmr979
Plant Genetics   Research Article

Isolation of genomic DNA from defatted oil seed residue of rapeseed (Brassica napus)

Authors: M. Sadia, M.A. Rabban, S. Hameed, S.R. Pearce and S.A. Malik

A simple protocol for obtaining pure, restrictable and amplifiable megabase genomic DNA from oil-free seed residue of Brassica napus, an important oil seed plant, has been developed. Oil from the dry seeds was completely recovered in an organic solvent and quantified gravimetrically followed by processing of the residual bioma.. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr1009
DOI:
10.4238/vol10-1gmr1009

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