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Molecular analysis of the SMN gene mutations in spinal muscular atrophy patients in China
Authors: W.L. Liu, F. Li, Z.X. He, R. Ai and H.W. Ma
Spinal muscular atrophy (SMA) is one of the most common autosomal recessive diseases. Survival motor neuron1 (SMN1) is the SMA disease-determining gene. We examined the molecular basis of SMA in 113 Chinese SMA patients. Homozygous exon 7 and 8 deletions in SMN1 were detected by PCR-RFLP. Heterozygous deletion of SMN1 was analyzed based on variation of th.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.13.4
- DOI:
- 10.4238/2013.September.13.4
Mutagenicity, genotoxicity, and scavenging activities of extracts from the soft coral Chromonephthea braziliensis: a possibility of new bioactive compounds
Authors: R.M. Carpes, B.G. Fleury, B.G. Lages, A.C. Pinto, C.A.F. Aiub and I. Felzenszwalb
Coral reefs are diverse ecosystems that have a high density of biodiversity leading to intense competition among species. These species may produce unknown substances, many with pharmacological value. Chromonephthea braziliensis is an invasive soft coral from the Indo-Pacific Ocean that is possibly transported by oil platforms and whose presence can be a .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.13.2
- DOI:
- 10.4238/2013.September.13.2
Chromosomal characteristics of a Brazilian whip spider (Amblypygi) and evolutionary relationships with other arachnid orders
Authors: E. Paula-Neto, D. Araujo, L.S. Carvalho, D.M. Cella and M.C. Schneider
We analyzed mitotic and meiotic cells of a Brazilian amblypygid, Heterophrynus longicornis, using conventional and molecular cytogenetic techniques (Giemsa staining, C-banding, Ag-NOR, and FISH with rDNA probe). This is the first study that focuses solely on amblypygid chromosomes; it was undertaken to add data on cytogenetic knowledge of this group and c.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.19.3
- DOI:
- 10.4238/2013.September.19.3
Potential role of Atp5g3 in epigenetic regulation of alcohol preference or obesity from a mouse genomic perspective
Authors: Y. Huang, L. Wang, B. Bennett, R.W. Williams, Y.J. Wang, W.K. Gu and Y. Jiao
The mitochondrial ATP synthase, subunit c, isoform 3 gene (Atp5g3) encodes subunit 9, the subunit of the multisubunit enzyme that catalyzes ATP synthesis during oxidative phosphorylation in mitochondria. According to the Ensembl database, Atp5g3 in mice is located on chromosome 2 between 73746504 and 73749383 bp, within the genomic regions of two sets of .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.18.1
- DOI:
- 10.4238/2013.September.18.1
Breeding of transgenic cattle for human coagulation factor IX by a combination of lentiviral system and cloning
Authors: P.S. Monzani, J.R. Sangalli, T.H.C. De Bem, F.F. Bressan, P. Fantinato-Neto, J.R.V. Pimentel, E.H. Birgel-Junior, A.M. Fontes, D.T. Covas and F.V. Meirelles
Recombinant coagulation factor IX must be produced in mammalian cells because FIX synthesis involves translational modifications. Human cell culture-based expression of human coagulation factor IX (hFIX) is expensive, and large-scale production capacity is limited. Transgenic animals may greatly increase the yield of therapeutic proteins and reduce costs... Read More»
- Genet. Mol. Res. 12(3):
- 2013.February.28.25
- DOI:
- 10.4238/2013.February.28.25
Development of polymorphic microsatellite markers for Dioscorea zingiberensis and cross-amplification in other Dioscorea species
Authors: Q.-Q. Yan, X.-Q. Sun, J.-L. Guo, Y.-Y. Hang and M.-M. Li
Dioscorea zingiberensis C.H. Wright (Dioscoreaceae) is an endemic species in central and southwestern China. In order to study the genetic diversity and population structure of this species, 19 novel polymorphic microsatellite loci were developed using a dual-suppression PCR technique. The number of alleles per locus ranged from 3 to 21, with an average o.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.19.10
- DOI:
- 10.4238/2013.September.19.10
Genetic structure of Aedes aegypti populations determined using pairwise comparisons
Authors: T. de F. Patarro, M.M. Guirado, L.M. Ravazzi and H.E.M. de C. Bicudo
The biological characteristics of Aedes aegypti (Diptera, Culicidae), which is a vector of dengue and yellow fever, make this organism a good model for studying population structure and the events that may influence it under the effect of human activity. We assessed the genetic variability of five A. aegypti populations using RAPD-PCR technique and six pr.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.19.9
- DOI:
- 10.4238/2013.September.19.9
Microsatellite markers reveal genetic divergence among wild and cultured populations of Chinese sucker Myxocyprinus asiaticus
Authors: W.W. Cheng, D.Q. Wang, C.Y. Wang, H. Du and Q.W. Wei
Studies of genetic diversity and genetic population structure are critical for the conservation and management of endangered species. The Chinese sucker Myxocyprinus asiaticus is a vulnerable monotypic species in China, which is at a risk of decline owing to fluctuations in effective population size and other demographic and e.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027581
- DOI:
- 10.4238/gmr.15027581
Xuemaitong granules attenuate carotid atherosclerosis by decreasing the expression of CD14+CD16+ monocytes, IL-6, TNF-�±, and hsCRP
Authors: Q. Zhang, G. Qian and Z. Ding
Carotid atherosclerosis (CAS) has been extensively studied because its position can be easily observed. Our objective was to investigate the effects of Xuemaitong granules on the generation and activation of CD14+CD16+ monocytes on the inflammatory reaction in CAS patients. In this study, 22 male apolipoprotein E (apoE)-defici.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.19
- DOI:
- 10.4238/2014.September.12.19
Composition and structure of microbial communities associated with different domestic sewage outfalls
Authors: Z.H. Wang, J.Q. Yang, D.J. Zhang, J. Zhou, C.D. Zhang, X.R. Su and T.W. Li
The diversity of microbiota in waste waters has not been thoroughly examined, despite the potential impact of microbes on effluent quality. Wastewater microbial communities harbor pathogenic bacteria, viruses, and parasites. To study microbial communities in domestic sewage outfalls, 454 pyrosequencing technology was used to i.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.21
- DOI:
- 10.4238/2014.September.12.21