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�²-casein gene expression by in vitro cultured bovine mammary epithelial cells derived from developing mammary glands
Authors: P.S. Monzani, F.F. Bressan, L.G. Mesquita, J.R. Sangalli and F.V. Meirelles
Epithelial cells from mammary gland tissue that are cultured in vitro are able to maintain specific functions of this gland, such as cellular differentiation and milk protein synthesis. These characteristics make these cells a useful model to study mammary gland physiology, development and differentiation; they can also be use.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1034
- DOI:
- 10.4238/vol10-2gmr1034
Prolactin-releasing peptide mRNA expression in mouse medulla remains relatively stable during pregnancy and lactation
Authors: H.S. Zhu, J.X. Du, Y.Y. Wang, L.F. Wang, L.Q. Hang, G.Y. Yang and Y.L. Wang
We compared levels of prolactin-releasing peptide (PrRP) mRNA expression in mouse medulla at different stages of pregnancy and lactation. Mouse medulla samples were collected on days 6, 12 and 18 of pregnancy and lactation, respectively (six per group), for mRNA. Expression levels of PrRP mRNA in the medulla were measured by s.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1086
- DOI:
- 10.4238/vol10-2gmr1086
Isolation and characterization of the organ-specific and light-inducible promoter of the gene encoding rubisco activase in potato (Solanum tuberosum)
Authors: D. Qu, Y. Song, W.M. Li, X.W. Pei, Z.X. Wang, S.R. Jia and Y.Q. Zhang
Constitutive promoters have been widely used in crop biotechnology applications. Tissue-specific or inducible promoters, however, have advantages in some cases. We isolated the 731-bp 5' flanking sequence of a potato (Solanum tuberosum) gene, encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase (RCA), wh.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1088
- DOI:
- 10.4238/vol10-2gmr1088
Serum ferritin and transferrin saturation levels in �²0 and �²+ thalassemia patients
Authors: I.F. Estev�£o, P. Peitl Junior and C.R. Bonini-Domingos
There have been few studies on the mutations that cause heterozygous beta-thalassemia and how they affect the iron profile. One hundred and thirty-eight individuals were analyzed, 90 thalasemic β0 and 48 thalasemic β+, identified by classical and molecular methods. Mutations in the hemochromatosis (HFE) gene, detecte.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1016
- DOI:
- 10.4238/vol10-2gmr1016
Simple and rapid protocol for the isolation of PCR-amplifiable DNA from medicinal plants
Authors: A.A. Alatar, M.A. Mahmoud, S.A. Al-Sohaibani and K.A. Abd-Elsalam
Medicinal plant species has a valuable economic importance because of its usage as pharmaceuticals, nutritional, as well as its use in popular medication. For DNA-based techniques, nanogram quantities of the purified DNA are requisite to amplify and yield sufficient amounts of PCR products. SDS-based DNA isolation method was u.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.13.1
- DOI:
- 10.4238/2012.February.13.1
Agronomic and molecular characterization of introgression lines from the interspecific cross Oryza sativa (BG90-2) x Oryza glumaepatula (RS-16)
Authors: P.N. Rangel, R.P.V. Brondani, P.H.N. Rangel and C. Brondani
The reduced genetic variability of modern rice varieties (Oryza sativa) is of concern because it reduces the possibilities of genetic gain in breeding programs. Introgression lines (ILs) containing genomic fragments from wild rice can be used to obtain new improved cultivars. The objective of the present study was to perform t.. Read More»
- Genet. Mol. Res. 7(1):
- vol7-1gmr406
- DOI:
- 10.4238/vol7-1gmr406
Discrimination of common bean cultivars using multiplexed microsatellite markers
Authors: P.C.B. Cardoso, C. Brondani, I.P.P. Menezes, P.A.M.R. Valdisser, T.C.O. Borba,, M.J. Del Peloso and R.P. Vianello
Analysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.24.1
- DOI:
- 10.4238/2014.March.24.1
Genetic structure based on nuclear and chloroplast microsatellite loci of Solanum lycocarpum A. St. Hil. (Solanaceae) in Central Brazil
Authors: K. Martins, L.J. Chaves, R. Vencovsky and P.Y. Kageyama
Solanum lycocarpum (Solanaceae) is a woody species found in the Brazilian Cerrado. The flowers are pollinated by Xylocopa spp bees, and seeds are dispersed by mammals with distinct home range sizes. As a consequence, relative contributions of pollen and seeds to overall gene flow can vary according to different spatial scales... Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1046
- DOI:
- 10.4238/vol10-2gmr1046
Molecular cloning and characterization of a tocopherol cyclase gene from Lactuca sativa (Asteraceae)
Authors: Y.L. Tang, W.W. Ren, L. Zhang and K.X. Tang
Tocopherol cyclase is a rate-limiting enzyme involved in tocopherol biosynthesis. The full-length cDNA encoding tocopherol cyclase (designated as LsTC) was cloned from lettuce (Lactuca sativa) for the first time by rapid amplification of cDNA ends (RACE) and characterized by means of quantitative RT-PCR. The full-length cDNA o.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1061
- DOI:
- 10.4238/vol10-2gmr1061
Optimization of a multiplex minisequencing protocol for population studies and medical genetics
Authors: Cl�¡udia M.B. Carvalho and S�©rgio D.J. Pena
Several technologically sophisticated high-throughput techniques have been recently developed for the study of human single nucleotide polymorphisms and the diagnosis of point mutations in human diseases. However, there is also a need for simple and inexpensive techniques suitable for clinical services and small research labor.. Read More»
- Genet. Mol. Res. 4(2):