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Association between the CYP11B2 gene Ã?Â?344T>C polymorphism and coronary artery disease: a meta-analysis
Authors: Y. Liu, H.L. Liu, W. Han, S.J. Yu and J. Zhang
Numerous studies have evaluated the association between the CYP11B2 gene Ã?Â?344T>C polymorphism and coronary artery disease (CAD) risk. However, the specific association is still controversial. To address this issue, PubMed, EMBASE, and China National Knowledge Infrastructure databases were searched for eligible articles that reported on the relations.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.22
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.22
Molecular analysis of apomixis in cassava
Authors: N.M.A. Nassar, D.C. Kalkmann and R. Collevatti
Cassava is the main staple for more than 800 million people in the tropics. It is propagated vegetatively by stem cuttings, which maintains superior genotypes but favors disease accumulation and spread. In this report, we present the results of the screening of the progeny and the second generation of the clone UnB 307 for apo.. Read More»
- Genet. Mol. Res. 5(3):
Identification of non-specific alkaline phosphatases in hyphal cells of the fungus Neurospora crassa by in situ histochemistry
Authors: G. Thedei Junior and A. Rossi
The present study was designed to identify alkaline phosphatases in non-permeabilized hyphal cells of the fungus Neurospora crassa by staining these enzymatic activities with a modified azo dye coupling method. Our strategy allowed the identification of three nonspecific alkaline phosphatase activities, one of them possibly be.. Read More»
- Genet. Mol. Res. 5(3):
Identification and mapping of resistance genes to Phakopsora pachyrhizi in soybean (Glycine max L.) accession PI 594767-A
Authors: G.A.F. Rocha, D.P. Alves, J.C. Oliveira and S.H. Brommonschenkel
The goal of this study was to study resistance inheritance in the soybean (Glycine max L.) accession PI 594767-A to the Phakopsora pachyrhizi isolate PPUFV02, and map the resistance gene(s) identified using microsatellite markers. Crosses between PI 594767-A and the susceptible cultivar ‘Conquista’ gave rise to the segregating subpopulations 2.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038475
- DOI:
- 10.4238/gmr.15038475
Mapping QTLs for drought tolerance in an F2:3 population from an inter-specific cross between Gossypium tomentosum and Gossypium hirsutum
Authors: J.Y. Zheng, G. Oluoch, M.K. Riaz Khan, X.X. Wang, X.Y. Cai, Z.L. Zhou, C.Y. Wang, Y.H. Wang, X.Y. Li, F. Liu and K.B. Wang
Cotton is one of the most important natural fiber crops in the world. Its growth and yield is greatly limited by drought. A quantitative trait locus (QTL) analysis was therefore conducted to investigate the genetic basis of drought tolerance in cotton (Gossypium spp) using 188 F2:3 lines developed from an inter-specific cross between a wild cotton species.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038477
- DOI:
- 10.4238/gmr.15038477
A rapid protocol for purification of total RNA for tissues collected from pigs at a slaughterhouse
Authors: V. M�©ndez, E. Avelar, A. Morales, M. Cervantes, A. Araiza and D. Gonz�¡lez
Since RNA extraction is a crucial step in many molecular techniques, the protocols for sample collection and RNA purification need to be adapted to optimize their performance when samples are collected from animals at commercial facilities. Here we provide an RNA purification protocol for animal tissues collected from slaughte.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.22.3
- DOI:
- 10.4238/2011.December.22.3
An improved K-means clustering method for cDNA microarray image segmentation
Authors: T.N. Wang, T.J. Li, G.F. Shao and S.X. Wu
Microarray technology is a powerful tool for human genetic research and other biomedical applications. Numerous improvements to the standard K-means algorithm have been carried out to complete the image segmentation step. However, most of the previous studies classify the image into two clusters. In this paper, we propose a novel K-means algorithm, which .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.3
- DOI:
- 10.4238/2015.July.14.3
Large-scale detection and application of expressed sequence tag single nucleotide polymorphisms in Nicotiana
Authors: Y. Wang, D. Zhou, S. Wang and L. Yang
Single nucleotide polymorphisms (SNPs) are widespread in the Nicotiana genome. Using an alignment and variation detection method, we developed 20,607,973 SNPs, based on the expressed sequence tag sequences of 10 Nicotiana species. The replacement rate was much higher than the transversion rate in the SNPs, and SNPs widely exist in the Nicotiana. In vitro .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.5
- DOI:
- 10.4238/2015.July.14.5
Manipulation of primer affinity improves high-resolution melting accuracy for imprinted genes
Authors: F.V.M. Rubatino, N.V. Carobin, M.L. Freitas, V.T. de Oliveira, R.X. Pietra, P.P.R. Oliveira, A.A. Bosco and F.S. Jehee
High-resolution melting (HRM) is considered an inexpensive, rapid, and attractive methodology for methylation analysis. In the application of the polymerase chain reaction (PCR) to methylation analysis, amplification efficiencies are biased towards unmethylated, rather than methylated, templates: a phenomenon known as PCR bias. To overcome PCR bias, prime.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.12
- DOI:
- 10.4238/2015.July.14.12
Expression differences in TEL-AML1 fusion gene in leukemia glucocorticoid-sensitive and -resistant cell lines
Authors: X.W. Wang and Y.H. Xu
We investigated the expression differences of the TEL-AML1 fusion gene in a leukemia glucocorticoid (GC)-sensitive cell line (CEM) and a GC-resistant cell line (Jurkat). Changes in TEL-AML1 expression before and after GC exposure were analyzed. Expression of GC-sensitive and GC-resistant leukemia cells following initial diagnosis and during treatment was .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.14
- DOI:
- 10.4238/2015.July.14.14