Salt
Analysis of genetic diversity of salt-tolerant alfalfa germplasms
Authors: J. Jiang, B.L. Yang, T. Xia, S.M. Yu, Y.N. Wu, H. Jin and J.R. Li
Random amplified polymorphic DNA technology was used to analyze the genetic diversity of 25 salt-tolerant alfalfa varieties using 30 different primers. Results showed that the percentage of polymorphic loci between single-plant DNA was 81.52%, and that between mixed DNA of various varieties was 61.65%. Compared to the mixed DNA samples, single-plant DNA s.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.4.1
- DOI:
- 10.4238/2015.May.4.1
Cloning and characterization of a SnRK2 gene from Jatropha curcas L
Authors: J. Chun, F.-S. Li, Y. Ma, S.-H. Wang and F. Chen
Although the SnRK2 class of Ser/Thr protein kinases is critical for signal transduction and abiotic stress resistance in plants, there have been no studies to examine SnRK2 in Jatropha curcas L. In the present study, JcSnRK2 was cloned from J. curcas using the rapid amplification of cDNA end technique and characterized. The JcSnRK2 genomic sequence is 257.. Read More»
- Genet. Mol. Res. 13(4):
- 2014
- DOI:
- 10.4238/2014
Comprehensive gene expression analysis of the DNA (cytosine-5) methyltransferase family in rice (Oryza sativa L.)
Authors: F. Ahmad, X. Huang, H.X. Lan, T. Huma, Y.M. Bao, J. Huang and H.S. Zhang
Cytosine DNA methylation is a conserved epigenetic regulatory mechanism in both plants and animals. DNA methyltransferases (DNA MTases) not only initiate (de novo) but also maintain the process of DNA methylation. Here, we characterized the genome-wide expression profiles of 10 cytosine DNA MTase genes belonging to 4 .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.7.9
- DOI:
- 10.4238/2014.July.7.9
Overexpression of NaKR3 enhances salt tolerance in Arabidopsis
Authors: Q. Luo1,2, Z. Zhao1, D.K. Li1, Y. Zhang1, L.F. Xie1, M.F. Peng1, S. Yuan3 and Y. Yang1,2
Salinity is a major abiotic stress in agriculture. Here, we report that SODIUM POTASSIUM ROOT DEFECTIVE3 (NaKR3), which encodes a heavy metal-associated domain protein, is involved in salt tolerance in Arabidopsis. The results of quantitative reverse transcription-polymerase chain reaction analysis revealed that NaKR3 was induced by high salinity and osmo.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15016378
- DOI:
- 10.4238/gmr.15016378
Molecular cloning and characterization of the full-length Hsp90 gene from Matricaria recutita
Authors: S.P. Ling, S.S. Su, H.M. Zhang, X.S. Zhang, X.Y. Liu, G.F. Pan and Y. Yuan
Heat shock protein 90 (Hsp90) is one of the most abundant and conserved chaperone proteins and plays important roles in plant growth and responses to environmental stimuli. However, little is known regarding the sequence and function of Hsp90s in Matricaria recutita. In the present study, we cloned the full-length cDNA sequence of the hsp90 gene from this.. Read More»
- Genet. Mol. Res. 13(4):
- 2014
- DOI:
- 10.4238/2014
Transgenic rice expressing a cassava (Manihot esculenta Crantz) plasma membrane gene MePMP3-2 exhibits enhanced tolerance to salt and drought stresses
Authors: Y. Yu1,2*, Y.C. Cui1*, C. Ren1,2, P.S.C.F. Rocha1, M. Peng3, G.Y. Xu4, M.L. Wang1 and X.J. Xia1
Plasma membrane proteolipid 3 (PMP3) is a class of small hydrophobic proteins found in many organisms including higher plants. Some plant PMP3 genes have been shown to respond to abiotic stresses and to participate in the processes of plant stress tolerance. In this study, we isolated the cassava (Manihot esculenta Crantz) MePMP3-2 gene and functionally c.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017336
- DOI:
- 10.4238/gmr.15017336
Ascorbate peroxidase from Jatropha curcas enhances salt tolerance in transgenic Arabidopsis
Authors: Y. Chen, J. Cai, F.X.Yang, B. Zhou and L.R. Zhou
Ascorbate peroxidase (APX) plays a central role in the ascorbate-glutathione cycle and is a key enzyme in cellular H2 O2 metabolism. It includes a family of isoenzymes with different characteristics, which are identified in many higher plants. In the present study, we isolated the APX gene from Jatropha curcas L, which is similar with other previously ch.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.20
- DOI:
- 10.4238/2015.May.11.20
Salt stress represses production of extracellular proteases in Bacillus pumilus.
Authors: R.F. Liu, C.L. Huang and H. Feng
Bacillus pumilus is able to secrete subtilisin-like proteases, one of which has been purified and characterized biochemically, demonstrating great potential for use in industrial applications. In the current study, the biosynthesis and transcription of extracellular proteases in B. pumilus (BA06) under salt stress were investigated using various methods, .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.27
- DOI:
- 10.4238/2015.May.11.27
Salicornia europaea L. Na+/H+ antiporter gene improves salt tolerance in transgenic alfalfa (Medicago sativa L.)
Authors: L.Q. Zhang, Y.D. Niu*, H. Huridu, J.F. Hao, Z. Qi and A. Hasi
In order to obtain a salt-tolerant perennial alfalfa (Medicago sativa L.), we transferred the halophyte Salicornia europaea L. Na+/H+ antiporter gene, SeNHX1, to alfalfa by using the Agrobacterium-mediated transformation method. The transformants were confirmed by both PCR and RT-PCR analyse.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.14
- DOI:
- 10.4238/2014.July.24.14
Random amplified polymorphic DNA analysis of salt-tolerant tobacco mutants generated by gamma radiation
Authors: Ã?Â?. Ã?Â?elik and Ã?Â?. Atak
Salinity is one of the major problems limiting the yield of agricultural products. Radiation mutagenesis is used to improve salt-tolerant mutant plants. In this study, we aimed to improve salt-tolerant mutants of two oriental tobacco varieties. One thousand seeds of each variety (M0) were irradiated with 100, 200, 300, and 400 Gy gamma rays by Cs-137 gamm.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.13.12
- DOI:
- 10.4238/2015.February.13.12