Rna Interference
Knock down of molt regulating gene for development control of Helicoverpa armigera
Authors: M.N. Sharif, M.S. Iqbal, R. Alam, B. Tabassum, M.F. Awan, Q. Ali and I.A. Nasir
RNA interference (RNAi) caused by exogenous double-stranded RNA (dsRNA) is a powerful tool, and to date is widely adopted to knockdown genetic targets crucial for growth and development of agriculturally important insect pests. Helicoverpa armigera is a pest feeding on more than 30 economically important crops worldwide and a major threat. Resistance to i.. Read More»
- Genet. Mol. Res. 19(2):
BmICE-2 interference vector construction and its silencing effect in the BmN-SWU1 cells
Authors: Hua-Shan Yi and Xianping Ma
Investigate the function of BmICE-2 gene in silkworm cells by constructing a BmICE-2 interference vector and analysing its interference effect. Online RNAi design software was used to predict the interference target sequence of Bmice-2. An optimized sequence was selected and inserted into the backbone of interference microRNA... Read More»
- Genet. Mol. Res. 16(4):
- gmr16039819
- DOI:
- 10.4238/gmr16039819
Segment-specific targeting via RNA interference mediates down-regulation of OPN expression in hepatocellular carcinoma cells
Authors: F. Lin1*, C.M. Huang2*, J. Cao1, Z.H. Pei1, W.L. Gu1, S.F. Fan1, K.P. Li1 and C.M. Lin1
Osteopontin (OPN) plays an important role in the metastasis and recurrence of tumors after resection of hepatocellular carcinoma (HCC). In this study, the down-regulation effect on OPN expression in HCC cells of RNA interference (RNAi) molecules designed to target different segments of OPN was investigated to identify a more effective site for OPN knockdo.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.6
- DOI:
- 10.4238/2015.November.18.6
Construction and identification of recombinant adenovirus carrying human TIMP-1shRNA geneg
Authors: Y.L. Sun, H. Xie, H.L. Lin, Q. Feng and Y. Liu
The aim of this study was to construct the recombinant adenovirus carrying human TIMP-1shRNA gene expression system for preliminary identification to lay the foundation for the further study of gene therapy. Using the Adeno-XTM system, the recombinant adenovirus plasmid pAdeno-XTM green fluorescent protein (GFP)-tissue inhibitor of metalloprotease (TIMP)-.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.16.3
- DOI:
- 10.4238/2015.January.16.3
Influence of suppression of CapG gene expression by siRNA on the growth and metastasis of human prostate cancer cells
Authors: B.K. Li, K. Guo, C.Y. Li, H.L. Li, P.P. Zhao,K. Chen,C.X. Liu
This study investigated CapG gene expression in prostate cancer cell lines; in addition, we explored the effects of CapG suppression on DU145 cell growth, and the underlying mechanism with which CapG affects DU145 cell growth and invasiveness. The expression of CapG and 18 related genes in DU145 cells was analyzed by flow cyto.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.1.28
- DOI:
- 10.4238/2015.December.1.28
Effects of VEGF suppression by small hairpin RNA interference combined with radiotherapy on the growth of cervical cancer
Authors: L. Qi, L.N. Xing, X. Wei and S.G. Song
This study aimed to investigate the inhibition of vascular endothelial growth factor (VEGF) by small hairpin RNA (shRNA) interference combined with radiotherapy on the growth of cervical cancer SiHa cell xenografts in nude mice. The effective pVEGF-shRNA plasmid was screened by reverse transcription-polymerase chain reaction (.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.7.2
- DOI:
- 10.4238/2014.July.7.2
RNA interference of leptin receptor in chicken adipocytes
Authors: A.X. Huang, J.J. Li, Y. Tian, J.D. Shen, Z.R. Tao, G.Q. Li, L.Z. Lu, Y. Fu and T.X. Wu
In this study, chicken adipocytes were cultured to evaluate RNA interference by the leptin receptor gene. A small interfering RNA of the leptin receptor gene was synthesized, with a suppression rate of 60% being generated (P peroxisome proliferator-activated receptor γ, fatty acid synthase, .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.5
- DOI:
- 10.4238/2014.August.7.5
Involvement of AP-1 in p38MAPK signaling pathway in osteoblast apoptosis induced by high glucose
Authors: Z.P. Feng, H.C. Deng, R. Jiang, J. Du and D.Y. Cheng
We investigated the effect of p38MAPK/AP-1 (activator protein-1) signaling on the apoptosis of osteoblasts induced by high glucose. A lentivirus vector of small hairpin RNA (shRNA) targeting p38MAPK was constructed in vitro. Osteoblasts MC3T3-E1 cultured in vitro were treated with vehicle, high glucose, p38MAPK-shRNA transfection, p38MAPK inhibitor, and u.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.26
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.26
Effects of AFP gene silencing on Survivin mRNA expression inhibition in HepG2 cells
Authors: Z.L. Fang, N. Fang, X.N. Han, G. Huang, X.J. Fu, G.S. Xie, N.R. Wang and J.P. Xiong
We investigated the effects of alpha-fetoprotein (AFP) gene silencing on Survivin expression in HepG2 cells. Small interfering RNA technology was used to downregulate AFP expression in HepG2 cells. An enzyme-linked immunosorbent assay was used to measure AFP concentration in the supernatant before and after transfection. An MTT assay was used to detect ce.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.30
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.30
Effects of NELL2 on the regulation of GnRH expression and puberty in female rats
Authors: S.S. Zhou and P. Li
Neural tissue-specific epidermal growth factor-like repeat domain-containing protein (NELL2) was previously found to play an important role in nerve growth, neural differentiation, neural elasticity, synaptic transport, and vesicle release. In this study, we examined the effect of NELL2 on gonadotropin-releasing hormone (GnRH).. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.12
- DOI:
- 10.4238/2014.August.28.12