Rna Interference
RNA interference-mediated silencing of the starch branching enzyme gene improves amylose content in rice
Authors: H.Y. Jiang, J. Zhang, J.M. Wang, M. Xia, S.W. Zhu and B.J. Cheng
Amylose and amylopectin are the 2 major components of plant storage starch. The rice starch branching enzyme (RBE) plays an important role in the starch components of rice. In the present study, we selected a specific 195-bp segment from the RBE3 gene to construct hairpin DNA, which was driven by an endosperm-specific high molecular weight glutenin promot.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.January.4.19
- DOI:
- 10.4238/2013.January.4.19
Effects of monomethoxypolyethylene glycol-chitosan nanoparticle-mediated dual silencing of livin and survivin genes in prostate cancer PC-3M cells
Authors: A.Q. Yang, P.J. Wang, T. Huang, W.L. Zhou and J. Landman
Monomethoxypolyethylene glycol-chitosan (mPEG-CS) nanoparticles were used as interfering RNA carriers to transfect human prostate cancer PC-3M cells to evaluate the effects of livin and survivin gene silencing on the proliferation and apoptosis. mPEG-CS nanoparticles with sizes of approximately 60 nm were first synthesized by .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027430
- DOI:
- 10.4238/gmr.15027430
Construction of recombinant adenoviruses carrying the optimal shRNA template against goat PTHrP and successful suppression of PTHrP expression in mammary epithelial cells
Authors: H.L. Zheng, X.M. Liu, Q. Yu, R.F. Xing, Z.Y. Yang and J. Luo
Parathyroid hormone-related protein (PTHrP) is a protein member of the parathyroid hormone family that regulates the dynamic balance between blood and bone calcium during lactation. However, the mechanism of its regulation is not very clear. In order to establish a framework for further functional studies of the PTHrP gene in goat mammary gland epithelial.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.4.2
- DOI:
- 10.4238/2013.September.4.2
S-phase kinase-associated protein 2 expression interference inhibits breast cancer cell proliferation
Authors: Y.J. Sun, X.K. Wang and B.J. Li
We investigated the expression of S-phase kinase-associated protein 2 (SKP2) in breast cancer tissues, and the effects of SKP2-specific small interfering RNA (siRNA) interference on breast cancer cell proliferation. Thirty subjects provided breast cancer tissue samples and 18 subjects provided normal breast specimens for this study. The expression of SKP2.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.10.4
- DOI:
- 10.4238/2015.August.10.4
Effect of siRNA silencing of inducible co-stimulatory molecule on myocardial cell hypertrophy after cardiac infarction in rats
Authors: W.M. Wang, Z. Liu and G. Chen
As the most common cardiac disease, myocardial infarction is followed by hypertrophy of cardiac myocytes and reconstruction of ventricular structure. The up-regulation of a series of factors including metalloproteinases, inflammatory factors, and growth factors after primary infarction lead to the hypertrophy, apoptosis, necro.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027338
- DOI:
- 10.4238/gmr.15027338
Effect of siRNA targeting HER2/neu on the proliferation and viability of prostate cancer PC-3M cells
Authors: C.Y. Liu, P.C. Xu, D.G. Chen, X.H. Fan, M.Q. Li, X. Yang and Y.P. Xu
The aim of this study was to investigate the effect of a small interfering RNA (siRNA) targeting human epidermal growth factor receptor 2 (HER2/neu) on the proliferation and viability of prostate cancer PC-3M cells. Chemically synthesized siRNA targeting HER2/neu was transfected into PC-3M cells by using liposomes, and cells transfected with empty liposom.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.14
- DOI:
- 10.4238/2015.December.16.14
N-ethylmaleimide-sensitive factor siRNA improves cardiac function following myocardial infarction in rats
Authors: Y. Zhou , Y. Liu, S.X. Yang and Z. Wang
This study examined the effects of N-ethylmaleimide-sensitive factor (NSF) small interfering RNA (siRNA) on cardiac function following myocardial infarction (MI) in rats. Thirty-six adult Sprague Dawley rats were randomly divided into three equivalent groups. An acute MI model was established by ligating the anterior descending branch of the left coronary.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.11
- DOI:
- 10.4238/2015.August.14.11