Molecular Markers
Interpretation of electrophoretograms of seven microsatellite loci to determine the genetic diversity of the Arabian Oryx
Authors: I.A. Arif, H.A. Khan, M. Shobrak, A.A. Al Homaidan, M. Al Sadoon, A.H. Al Farhan and A.H. Bahkali
Microsatellite markers are commonly used for examining population structure, especially inbreeding, outbreeding and gene flow. An array of microsatellite loci, preferably with multiallelic presentation, is preferable for ensuring accurate results. However, artifact peaks or stutters in the electrophoretograms significantly ham.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr714
- DOI:
- 10.4238/vol9-1gmr714
Genetic diversity studies in pea (Pisum sativum L.) using simple sequence repeat markers
Authors: P. Kumari, N. Basal, A.K. Singh, V.P. Rai, C.P. Srivastava and P.K. Singh
The genetic diversity among 28 pea (Pisum sativum L.) genotypes was analyzed using 32 simple sequence repeat markers. A total of 44 polymorphic bands, with an average of 2.1 bands per primer, were obtained. The polymorphism information content ranged from 0.657 to 0.309 with an average of 0.493. The variation in genetic diversity among these cultivars ran.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.March.13.12
- DOI:
- 10.4238/2013.March.13.12
A molecular marker for in situ genetic resource conservation of Capsicum annuum var. acuminatum (Solanaceae)
Authors: N. Kaewdoungdee and T. Tanee
The Thailand cultivar pepper ‘phrik man bangchang’ (Capsicum annuum var. acuminatum, Solanaceae) was originally cultivated in the Bangchang Subdistrict, Amphawa District in Samut Songkhram Province. The cultivated areas are limited; we verified its distribution in Thailand for in situ ‘phrik man bangchang’ genetic resource conserva.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.February.28.10
- DOI:
- 10.4238/2013.February.28.10
Initial assessment of natural diversity in Mexican fig landraces
Authors: M.T. Garc�?a-Ruiz, V.M. Mendoza-Castillo, E. Valadez-Moctezuma and A. Muratalla-L�?ºa
The common fig (Ficus carica L.) was introduced into Mexico by Spanish Franciscan missionaries in the 16th century. It is widely assumed that Mexican figs are the Spanish cultivar Black Mission. We collected and propagated 12 fig plants from six landraces from different states in Central Mexico that represent different climate. All of them were grown in a.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.12
- DOI:
- 10.4238/2013.September.23.12
Molecular characterization of endophytes isolated from Saccharum spp based on esterase and ribosomal DNA (ITS1-5.8S-ITS2) analyses
Authors: A.C. Leme, M.R.R. Bevilaqua, S.A. Rhoden, C.A. Mangolin, M.F.P.S. Machado and J.A. Pamphile
This study used esterases and ribosomal DNA (rDNA) markers to determine endophytic variability in order to better understand endophyte-host interactions. Polyacrylamide gel electrophoresis and esterase isoenzymes (EST; EC 3.1.1.3), with a-naphthyl acetate and β-naphthyl acetate as substrates, were used to assess relationships among endophytes. ITS1-5.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.11
- DOI:
- 10.4238/2013.September.27.11
Construction of an integrated genetic map for Capsicum baccatum L.
Authors: M.M. Moulin, R. Rodrigues, H.C.C. Ramos, C.S. Bento, C.P. Sudr�?©, L.S.A. Gon�?§alves and A.P. Viana
Capsicum baccatum L. is one of the five Capsicum domesticated species and has multiple uses in the food, pharmaceutical and cosmetic industries. This species is also a valuable source of genes for chili pepper breeding, especially genes for disease resistance and fruit quality. However, knowledge of the genetic structure of C. baccatum is limited. A refer.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.12
- DOI:
- 10.4238/2015.June.18.12
Effect of two single nucleotide polymorphisms on milk yield and composition
Authors: J.C. Rinc�?³n, A. L�?³pez-Herrera and J.J. Echeverri
The bovine prolactin (PRL) and bovine growth hormone (bGH) genes exhibit several polymorphisms. Some of them can be detected by molecular techniques using restriction endonucleases, such as RsaI for the PRL gene and MspI for the bGH gene. We examined the relationship between the PRL-RsaI and bGH-MspI polymorphisms and some economically important character.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.15
- DOI:
- 10.4238/2013.April.2.15
Genetic diversity analysis of the Uruguayan Creole cattle breed using microsatellites and mtDNA markers
Authors: E. Armstrong, A. Iriarte, A.M. Mart�?nez, M. Feijoo, J.L. Vega-Pla, J.V. Delgado and A. Postiglioni
The Uruguayan Creole cattle population (N = 600) is located in a native habitat in south-east Uruguay. We analyzed its genetic diversity and compared it to other populations of American Creole cattle. A random sample of 64 animals was genotyped for a set of 17 microsatellite loci, and the D-loop hyper-variable region of mtDNA was sequenced for 28 calves o.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.7
- DOI:
- 10.4238/2013.April.10.7
Evaluation of genetic diversity in fig accessions by using microsatellite markers
Authors: A.D.B. do Val, C.S. Souza, E.A. Ferreira, S.M.L. Salgado, M. Pasqual and G.M.A. Can�?§ado
Fig (Ficus carica L.) is a fruit of great importance worldwide. Its propagation is carried out with stem cuttings, a procedure that favors the occurrence of synonymy among specimens. Thus, molecular markers have become an important tool for studies of DNA fingerprinting, germplasm characterization, and genetic diversity evaluation in this plant species. T.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.25.9
- DOI:
- 10.4238/2013.April.25.9
SSR-based association mapping of salt tolerance in cotton (Gossypium hirsutum L.)
Authors: Y.L. Zhao, H.M. Wang, B.X. Shao, W. Chen, Z.J. Guo, H.Y. Gong,X.H. Sang, J.J. Wang and W.W. Ye
The identification of simple sequence repeat (SSR) markers associated with salt tolerance in cotton contributes to molecular assisted selection (MAS), which can improve the efficiency of traditional breeding. In this study, 134 samples of upland cotton cultivars were selected. The seedling emergence rates were tested under 0.3.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027370
- DOI:
- 10.4238/gmr.15027370