Microsatellite Markers
Development and characterization of microsatellite markers via cross-species amplification of Paramisgurnus dabryanus
Authors: G. Chen, S. Huang, J. Gao, X. Bai, W. Wang and X. Cao
The large-scale loach, Paramisgurnus dabryanus, is a small freshwater fish of major economic importance in many Asian countries, particularly China and South Korea. Fifteen polymorphic microsatellite (simple sequence repeat) markers were obtained through cross-species amplification between this loach and a related species, Misgurnus anguillicaudatus (GenB.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.1
- DOI:
- 10.4238/2015.May.29.1
Recycling of superfine resolution agarose gel
Authors: T.-Y. Seng, R. Singh, Q.Z. Faridah, S.-G. Tan and S.S.R.S. Alwee
Genetic markers are now routinely used in a wide range of applications, from forensic DNA analysis to marker-assisted plant and animal breeding. The usual practice in such work is to extract the DNA, prime the markers of interest, and sift them out by electrically driving them through an appropriate matrix, usually a gel. The gels, made from polyacrylamid.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.March.11.1
- DOI:
- 10.4238/2013.March.11.1
Transferability of microsatellite markers among economically and ecologically important galliform birds
Authors: N. Bech, C. Novoa, J.F. Allienne and J. Boissier
We used the partially sequenced genomes of the turkey and chicken to find a large number of microsatellite markers. We then characterized 10 polymorphic microsatellite markers developed by cross-species amplification from economically and ecologically important birds to various European sub-species of the grey partridge. Even .. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr760
- DOI:
- 10.4238/vol9-2gmr760
Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest
Authors: M. Novello, J.F. Rodrigues, F. Pinheiro, G.C.X. Oliveira, E.A. Veasey and S. Koehler
Microsatellite markers were developed for the endangered Brazilian orchid species Cattleya coccinea to describe its genetic diversity and structure and to support conservation studies. Nine microsatellite loci were isolated and characterized using an enriched genomic library. All loci are polymorphic at least in the 2 populations sampled, except for loci .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.3.3
- DOI:
- 10.4238/2013.September.3.3
Genetic variability in Melipona scutellaris from Rec�´ncavo, Bahia, Brazil
Authors: J.L. Viana, A.K. Francisco, C.A.L. Carvalho and A.M. Waldschmidt
Bees play a key role in pollination and thereby help maintain plant diversity. The stingless bee Melipona scutellaris is an important pollinator in northeastern Brazil because it is endemic to this region. Both deforestation and timber harvesting have reduced the nesting sites for this species, thus reducing its population and range. Genetic studies may h.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.10.1
- DOI:
- 10.4238/2013.September.10.1
Cross-species transferability of eastern white pine (Pinus strobus) nuclear microsatellite markers to five Mexican white pines
Authors: A.R. Villalobos-Ar�¡mbula, J.A. P�©rez de la Rosa, A. Arias, O.P. Rajora
We examined cross-species transferability and usefulness of six nuclear microsatellite markers developed in consubgeneric eastern white pine (Pinus strobus) with regard to ecologically and commercially important Mexican white pine species of conservation genetics concern: Pinus chiapensis (Mart.) Andresen, P... Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.24
- DOI:
- 10.4238/2014.September.12.24
Population structure of jatropha and its implication for the breeding program
Authors: D.N. Santos, J.L. Ferreira, M. Pasqual, A.L. Generoso3, T.A. Setotaw, G.M.A. Can�§ado and W.A. Vendrame
Jatropha (Jatropha curcas L.) has potential as an oilseed crop that requires the development of technology for its exploitation. The objective of this study was to assess the population structure and the genetic diversity in jatropha accessions at a global level using simple sequence repeat (SSR) molecular markers. Jatropha accessions (N = 109) from 10 co.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017770
- DOI:
- 10.4238/gmr.15017770
Genetic variability in Blanco Orejinegro breed cattle populations in Colombia
Authors: R. MartÃ?Ânez, A.P. LlinÃ?¡s and J.F. M.-Rocha
We analyzed population structure and genetic diversity in Blanco Orejinegro Creole cattle with 12 microsatellite markers, genotyping 138 individuals belonging to 7 commercial and 3 conservation herds. These markers showed a high level of polymorphism; 171 alleles were identified. The mean number of alleles per locus was 5.63 (3.82-6.58). The total number .. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.4
- DOI:
- 10.4238/2013.April.10.4
Verification and characterization of chromosome duplication in haploid maize
Authors: E.G. de Oliveira Couto, E.V. Resende Von Pinho, R.G. Von Pinho, A.D. Veiga, M.R. de Carvalho, F. de Oliveira Bustamante and M.S. Nascimento
Doubled haploid technology has been used by various private companies. However, information regarding chromosome duplication methodologies, particularly those concerning techniques used to identify duplication in cells, is limited. Thus, we analyzed and characterized artificially doubled haploids using microsatellites molecular markers, pollen viability, .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.26.9
- DOI:
- 10.4238/2015.June.26.9
Isolation and characterization of microsatellite markers for Osmanthus fragrans (Oleaceae) using 454 sequencing technology
Authors: W.J. Yuan, Y. Li, Y.F. Ma, Y.J. Han and F.D. Shang
Osmanthus fragrans (Oleaceae) is an evergreen shrub or small tree that grows in south China. In this study, Roche 454 FLX+ sequencing combined with the magnetic bead enrichment method was used to isolate microsatellite markers from the genome of O. fragrans. A total of 1471 microsatellites that contained enough flanking sequences for primer pair design we.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.15
- DOI:
- 10.4238/2015.December.16.15