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Microsatellite Markers

Medical Genetics   Research Article

Development of microsatellite markers of vandaceous orchids for species and variety identification

Authors: S. Peyachoknagul, C. Nettuwakul, P. Phuekvilai, S. Wannapinpong and K. Srikulnath

Vandaceous orchids are a group of orchid genera in the subfamily Vandoideae. Among this group, Mokara, Phalaenopsis, and Vanda are the most popular and commercially important orchids in Thailand. Novel microsatellite markers were developed from Mokara, the intergeneric hybrid from 3 genera Vanda.. Read More»

Genet. Mol. Res. 13(3):
2014.July.24.23
DOI:
10.4238/2014.July.24.23
Animal Genetics   Research Article

Genetic diversity and genetic structure of consecutive breeding generations of golden mandarin fish (Siniperca scherzeri Steindachner) using microsatellite markers

Authors: X.N. Luo, M. Yang, X.F. Liang, K. Jin, L.Y. Lv, C.X. Tian, Y.C. Yuan and J. Sun

In this study, 12 polymorphic microsatellites were investigated to determine the genetic diversity and structure of 5 consecutive selected populations of golden mandarin fish (Siniperca scherzeri Steindachner). The total numbers of alleles, average heterozyosity, and average polymorphism information content showed that the genetic diversity of these breed.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.25.1
DOI:
http://dx.doi.org/10.4238/2015.September.25.1
Animal Genetics   Research Article

Isolation and characterization of novel microsatellite markers from the sika deer (Cervus nippon) genome

Authors: Y.M. Li, C.Y. Bai, W.P. Niu, H. Yu, R.J. Yang, S.Q. Yan, J.Y. Zhang, M.J. Zhang and Z.H. Zhao

Microsatellite markers are widely and evenly distributed, and are highly polymorphic. Rapid and convenient detection through automated analysis means that microsatellite markers are widely used in the construction of plant and animal genetic maps, in quantitative trait loci localization, marker-assisted selection, identification of genetic relationships, .. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.September.28.4
DOI:
http://dx.doi.org/10.4238/2015.September.28.4
Plant Genetics   Research Article

Genetic diversity of Qatari date palm using SSR markers

Authors: K. Elmeer and I. Mattat

The genetic diversity in the date palm germplasm of 59 female accessions representing 12 cultivars from different locations in Qatar was investigated using 14 loci of simple-sequence repeat (SSR) primers. A total of 94 alleles, with a mean of 6.7 alleles per locus, were scored. The number of alleles per locus varied from 3 (primer mPdCIR090) to 11.. Read More»

Genet. Mol. Res. 14(1):
2015.March.6.9
DOI:
10.4238/2015.March.6.9
Plant Genetics   Research Article

Molecular and agronomic analysis of intraspecific variability in Capsicum baccatum var. pendulum accessions

Authors: P.S.S. Leite, R. Rodrigues, R.N.O. Silva, S. Pimenta, A.M. Medeiros, C.S. Bento and L.S.A. Gon�§alves

Capsicum baccatum is one of the most important chili peppers in South America, since this region is considered to be the center of origin and diversity of this species. In Brazil, C. baccatum has been widely explored by family farmers and there are different local names for each fruit phenotype, such as cambuci and dedo-de-mo&.. Read More»

Genet. Mol. Res. 15(4):
gmr.15048482
DOI:
10.4238/gmr.15048482
Microbial Genetics   Research Article

Isolation and characterization of polymorphic microsatellite loci for Pachycrepoideus vindemmiae (Rondani) (Hymenoptera: Pteromalidae)

Authors: W. Chen, L. Fang, J.L. Liu, Z. He and H.Y. Hu

The parasitoid wasp Pachycrepoideus vindemmiae (Rondani) is a common pupal parasitoid of many fly pests that is distributed worldwide. This organism can be used for biological control in orchards or livestock farms. Identifying polymorphic microsatellite loci would be useful for analyzing the population genetic structure of the parasitoid. In the current .. Read More»

Genet. Mol. Res. 14(1):
2015.March.13.7
DOI:
10.4238/2015.March.13.7
Plant Genetics   Research Article

Analysis of transferability of microsatellite primers (SSR) in wild Passiflora species and intraspecific genetic diversity in Passiflora alata

Authors: M.A.A. Silva, M.M. Souza, G.S. Silva, C.A.F. Melo, R.X. Corr�ªa, I.S. Ara�ºjo and L.D.H.C.S. Concei�§�£o

The genus Passiflora L. is the most representative of Passifloraceae, with over 500 known species, among which 150-200 originated from Brazil. In addition to the great commercial importance of this genus for the fruit market, many of the species have exotic flowers with a huge diversity of colors and can thereby be ex.. Read More»

Genet. Mol. Res. 13(3):
2014.August.7.6
DOI:
10.4238/2014.August.7.6
Animal Genetics   Research Article

Detection of genetic polymorphism among and within Echinococcus granulosus strains by heteroduplex analysis of a microsatellite from the U1 snRNA genes

Authors: P.A. Roratto, M.L. Bartholomei-Santos, A.M. Gutierrez, L. Kamenetzky, M.C. Rosenzvit and A. Zaha

Polymerase chain reaction of a pentanucleotide microsatellite in the U1 snRNA gene complex generated a multiple band pattern due to the priming of paralogous sequences. Denaturation and slow renaturation of polymerase chain reaction products allow the formation of heteroduplex DNA that can be detected by its differential mobil.. Read More»

Genet. Mol. Res. 5(3):
Plant Genetics   Research Article

Development and characterization of polymorphic microsatellite markers for Castanopsis hystrix (Fagaceae)

Authors: Y. Jiang, Z.H. Li, J.Y. Zhu and H.L. Liu

Castanopsis hystrix is one of the most important and dominant species in evergreen broad-leaved forests in subtropical China. However, the population of this species undergone severe decline because of deforestation over the past 2 decades. For both conservation and forestry management, it is essential to develop molecular markers for C. hystrix. We ident.. Read More»

Genet. Mol. Res. 14(1):
http://dx.doi.org/2015.March.30.1
DOI:
http://dx.doi.org/10.4238/2015.March.30.1
Animal Genetics   Short communication

Development and testing of 13 polymorphic microsatellite markers in Larimichthys polyactis (Sciaenidae) using 5' anchored PCR

Authors: C.Y. Ma, H.Y. Ma and L.B. Ma

Larimichthys polyactis is a commercially important marine fish species in southeast Asia. The population crashed due to overfishing in the 1970s, but has since recovered. We developed 13 novel polymorphic microsatellite markers in L. polyactis using 5' anchored PCR. The characteristics of these loci were estimated by analyzing.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1464
DOI:
10.4238/vol10-3gmr1464