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Human Genetics   Research Article

Differential expression of trypsin-3 and phosrestin II genes in the main malaria vector, Anopheles darlingi, from the Brazilian Amazon region

Authors: Ohse, K.C, Matiolli, C.C, Scarpassa, V.M, Santos, V. S, Tadei, W. P, Rafael, M. S

Anopheles darlingi is the most anthropophilic mosquito related to Plasmodium infection of malaria, causing significant morbidity and mortality in South America. Pyrethroid chemical has been used to control mosquitos. We analyzed the expression of trypsin-3 and phosrestin II genes implicated to feeding and resistance to insecti.. Read More»

Genet. Mol. Res. 16(4):
Animal Genetics   Research Article

New microsatellite markers for the neotropical malaria vector Anopheles nuneztovari sensu lato A.S.

Authors: A.S. Cunha-Machado and V.M. Scarpassa

Anopheles nuneztovari sensu lato consists of cryptic species and genetic lineages, one of which is an important human malaria vector in the northern part of South America. Population structure and evolutionary genetics studies may help in the definition and delimitation of the species and lineages within this species complex, which is relevant information.. Read More»

Genet. Mol. Res. 13(4):
Human Genetics   Research Article

Prevalence of Plasmodium falciparum and P. vivax in an area of transmission located in Pará State, Brazil, determined by amplification of mtDNA using a real-time PCR assay

Authors: C.R.T. Souza T.A.A. Carvalho R.C.G. Amaral L.S. Cunha M.G. Cunha J.F. Guerreiro

The need for a more sensitive and time-efficient assay for malaria has led to the development of molecular assays involving real-time PCR (qPCR), a procedure that has the potential to detect low levels of parasitemia, identify mixed infections, and allow for precise differentiation of species via melting curve analysis or TaqMan fluorescence-labeled probe.. Read More»

Genet. Mol. Res. 11(3):
Human Genetics   Research Article

Genetic polymorphisms in TLR4, CR1 and Duffy genes are not associated with malaria resistance in patients from Baixo Amazonas region, Brazil

Authors: S.C. Soares1,4, K. Abé-Sandes5,6, V.B. Nascimento Filho4, F.M.F. Nunes2 and W.A. Silva Jr.2,3

The main purpose of this research was to analyze the relation of the genetic polymorphisms frequently expressed by antigen-presenting cells, erythrocytes and malaria susceptibility/resistance with the human malaria infection cases. The sample used consisted of 23 Plasmodium vivax (Pv)- and P. falciparum (Pf)-infected patients, and 21 healthy individuals a.. Read More»

Genet. Mol. Res. 7(4):
Cytogenomics   Research Article

Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species

Authors: G.M. Guimarães-Marques J.S. Batista H.M. Guimarães M.V. Naice-Daou M.P. Lima K.M. Formiga J.M.M. Santos C.A.P. Lima M.S. Rafael

The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity rang.. Read More»

Genet. Mol. Res. 11(4):
Animal Genetics   Research Article

Genetic differences based on AFLP markers in the mosquito species Anopheles darlingi collected in versus near houses in the region of Porto Velho, RO, Brazil

Authors: L.M. Santos, R.A. Gama, A.E. Eiras and C.G. Fonseca

Anopheles darlingi is the most important malaria vector in Central and South America. After a dramatic reduction of malaria cases in the whole Brazilian territory, with the lowest abundance being attained by 1970, the disease resurged in the Amazon region, where it is now a great public health concern. Consequently, better kno.. Read More»

Genet. Mol. Res. 9(4):
Human Genetics   Research Article

Frequency of ABO blood group system polymorphisms in Plasmodium falciparum malaria patients and blood donors from the Brazilian Amazon region

Authors: D.B. Carvalho, L.C. de Mattos, W.C. Souza-Neiras, C.R. Bonini-Domingos, A.B. Cósimo, L.M. Storti-Melo, G.C. Cassiano, A.A.A. Couto, A.J. Cordeiro, A.R.B. Rossit and R.L.D. Machado

We investigated the ABO genotypes and heterogeneity of the O alleles in Plasmodium falciparum-infected and non-infected individuals from the Brazilian Amazon region. Sample collection took place from May 2003 to August 2005, from P. falciparum malaria patients from four endemic regions of the Brazilian Amazon. The control grou.. Read More»

Genet. Mol. Res. 9(3):
Plant Genetics   Research Article

Molecular differentiation in natural populations of Anopheles oswaldoi sensu lato (Diptera: Culicidae) from the Brazilian Amazon, using sequences of the COI gene from mitochondrial DNA

Authors: V.M. Scarpassa and J.E. Conn

Anopheles (Nyssorhynchus) oswaldoi (Peryassú, 1922) s. l., which has been incriminated as a potential human malaria vector in Western Brazilian Amazon, may constitute a cryptic species complex. However, the most recent study with isozymes indicated high similarity among samples from the States of Acre, Amazonas and Rond.. Read More»

Genet. Mol. Res. 5(3):
Animal Genetics   Research Article

Karyotype of Brazilian Anopheles albitarsis sensu lato (Diptera:Culicidae)

Authors: Míriam Silva Rafael, Ivanildo Pereira dos Santos-Junior, Wanderli Pedro Tadei, Maria Anice Mureb Sallum and Oswaldo Paulo Forattini

Anopheles (Nyssorhynchus) albitarsis sensu lato is an important malaria vector in Brazil, especially in the Brazilian Amazon region. Chromosome preparations of fourth-instar larvae of A. albitarsis from Iranduba and Coari (AM) and Ilha Comprida (SP) were analyzed for karyotype determination and to improve cytogenetic identific.. Read More»

Genet. Mol. Res. 4(4):
Medical Genetics   Research Article

Frequency of the HFE C282Y and H63D polymorphisms in Brazilian malaria patients and blood donors from the Amazon region

Authors: F.R. Torres, W.C. Souza-Neiras, A.A. D’Almeida Couto,V.S.C. D’Almeida Couto, C.E. Cavasini, A.R.B. Rossit, R.L.D. Machado and C.R. Bonini-Domingos

Malaria is an endemic parasitosis and its causitive agent, Plasmodium, has a metabolism linked to iron supply. HFE is a gene with the polymorphisms C282Y and H63D, which are associated with a progressive iron accumulation in the organism leading to a disease called hereditary hemochromatosis. The aim of the present study was to determine the allelic and g.. Read More»

Genet. Mol. Res. 7(1):