All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Differential expression of trypsin-3 and phosrestin II genes in the main malaria vector, Anopheles darlingi, from the Brazilian Amazon region

Author(s): Ohse, K.C, Matiolli, C.C, Scarpassa, V.M, Santos, V. S, Tadei, W. P, Rafael, M. S

Anopheles darlingi is the most anthropophilic mosquito related to Plasmodium infection of malaria, causing significant morbidity and mortality in South America. Pyrethroid chemical has been used to control mosquitos. We analyzed the expression of trypsin-3 and phosrestin II genes implicated to feeding and resistance to insecticides, immune response and sensory antenna mechanisms, respectively, of larvae and adult of A. darlingi, through quantitative reverse transcription polymerase chain reaction (qRT-PCR). We aimed to validate the similarity in nucleotide sequences of A. darlingi RNA sequencing libraries by in silico, and qRT- PCR, owing to their possible effects on the ability to spread disease. The expression of trypsin-3 and phosrestin II was higher in the first and second instar larvae as compared with that in adults. These differentially expressed trypsin-3 and phosrestin II genes do not provide us an evidence that both genes participate in pyrethroid resistance. The signaling pathway involving both genes requires further study. Preliminary phylogenetic relationships and the accumulation of mutations analysis in both genes were also compared with trypsin and phosrestin sequences of 15 and 17 other anopheline species, respectively, to obtain a mutational rate of 0.02 on phylogenetic trees. Trypsin gene of A. darlingi and A. albimanus clustered into the same group and was distinct from the species of A. gambiae complex and other anopheline. For phosrestin II, A. darlingi was separated from the remaining species from Africa, Asia, and Europe. Although the groups showed low to moderate support, it is possible to infer that both genes may belong to two evolutionary groups: one present in the anopheline species of New World and other in the anopheline species of Old World, and be useful for future studies


Stay informed on our latest news!