
Authors: E.J. Oliveira, O.S. Oliveira Filho and V.S. Santos
We evaluated the genetic variation of cassava accessions based on qualitative (binomial and multicategorical) and quantitative traits (continuous). We characterized 95 accessions obtained from the Cassava Germplasm Bank of Embrapa Mandioca e Fruticultura; we evaluated these accessions for 13 continuous, 10 binary, and 25 multicategorical traits. First, we.. Read More»
Authors: D.S. Rocha, C.P. Santos, M.M. Bajay, J.B. Campos, A.F. Blank,J.B. Pinheiro and M.I. Zucchi
Microsatellite primers were developed and optimized for Lippia alba to characterize the L. alba germplasm bank of Universidade de SÃ?£o Paulo. A genomic library enabled the design of 9 microsatellite primers. Six of the 9 primers yielded polymorphic products, which defined 2 groups in the bank. The data provide support to characterize germplasm banks, genet.. Read More»
Authors: I.A. Giantsis N. Kravva A.P. Apostolidis
Despite the great commercial and economic importance of mussels, Mytilus galloprovincialis (Bivalvia, Mollusca) in Greece, little information is available concerning their population genetic structure. We used RAPD markers to examine genetic differentiation and potential impact of aquaculture practices and other anthropogenic activities on the genetic str.. Read More»
Authors: J.A.F. Diniz-Filho1, J.V.B.P.L. Diniz2 and M.P.C. Telles3
Conservation strategies routinely use optimization methods to identify the smallest number of units required to represent a set of features that need to be conserved, including biomes, species, and populations. In this study, we provide R scripts to facilitate exhaustive search for solutions that represent all of the alleles in networks with the smallest .. Read More»
Authors: X.-L. Wang, Y. Li, L. Liao, C.-J. Bai and Z.-Y. Wang
Axonopus compressus (Sw.) Beauv. is a perennial herb widely used as a garden lawn grass. In this study, we used Roche 454 pyrosequencing, combined with the magnetic bead enrichment method FIASCO, to isolate simple sequence repeat markers from the A. compressus genome. A total of 1942 microsatellite loci were identified, with 53,193 raw sequencing reads. O.. Read More»
Authors: Z.-H. Liu, Q. Xie and Z.-Q. Li
Pinus tabulaeformis f. shekanensis is a rare taxon endemic in the Ziwuling Loess Plateau, of which only one population is known. Inter-simple sequence repeat molecular markers were employed to compare the taxon’s genetic diversity with its 4 nearest wild relatives (P. tabulaeformis, P. tabulaeformis var. mukdensis, P. massoniana, and P. henryi) to a.. Read More»
Authors: H.S. An J.W. Lee J.Y. Park
Populations of the Pacific abalone, Haliotis discus hannai, have been severely overexploited over the past few decades in Korea. Information regarding the levels of genetic variability and structure within populations is insufficient for the development of effective strategies for conservation of genetic diversity of this species. To assess the genetic statu.. Read More»
Authors: J.L. Tu, M.J. Zhang, X.Q. Wang, X.L. Zhang and Z.X. Lin
The genetic diversity of 51 upland cotton cultivars with different parental origins and breeding periods that were developed in Hubei Province was studied on the basis of 237 mapped simple sequence repeat markers covering the cotton genome. A total of 108 polymorphic primer pairs amplified 196 loci; the polymorphism information content range was 0.04 to 0.83.. Read More»
Authors: Iqbal A, Khan AS, Khan IA, Awan FS, Ahmad A and Khan AA
The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polym.. Read More»
Authors: Y.-E. Yun, J.-N. Yu, G.H. Nam, S.-A. Ryu, S. Kim, K. Oh and C.E. Lim
We used next-generation sequencing to develop 9 novel microsatellite markers in Aconitum austrokoreense, an endemic and endangered medicinal plant in Korea. Owing to its very limited distribution, over-harvesting for traditional medicinal purposes, and habitat loss, the natural populations are dramatically declining in Korea. All novel microsatellite mark.. Read More»