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Gene

Plant Genetics   Research Article

High-throughput, low-cost, and event-specific polymerase chain reaction detection of herbicide tolerance in genetically modified soybean A2704-12

Authors: H. Ma, H. Li, J. Li, X.F. Wang, P.C. Wei, L. Li and J.B. Yang

The aim of this study was to develop an event-specific qualitative and real-time quantitative polymerase chain reaction (PCR) method for detection of herbicide-tolerance genetically modified (GM) soybean A2704-12. The event-specific PCR primers were designed, based on the 5'-flanking integration sequence in the soybean genome,.. Read More»

Genet. Mol. Res. 13(1):
2014.January.28.14
DOI:
10.4238/2014.January.28.14
Animal Genetics   Research Article

Pedigree analyses in the Breeding Program for Nellore Cattle

Authors: Vozzi PA, Marcondes CR, Bezerra LAF and Lobo RB

Parameters based on the probability of gene origin were used to describe genetic variability in three reproductive groups from the Breeding Program for Nellore Cattle (PMGRN). The three reproductive populations (cows in reproductive age, bulls from artificial insemination centers and young bulls in progeny test) generated medi.. Read More»

Genet. Mol. Res. 6(4):
Animal Genetics   Research Article

A missense mutant of the PPAR-�³gene associated with carcass and meat quality traits in Chinese cattle breeds

Authors: Y.Y. Fan G.W. Fu C.Z. Fu L.S. Zan W.Q. Tian

The peroxisome proliferator-activated receptor gamma (PPAR-Ã?³) is a key molecule in adipocyte differentitation; it transactivates multiple target genes in lipid metabolic pathways. Using PCR-SSCP and DNA sequencing, we evaluated a potential association of an SNP (72472 Gï¹¥T in exon7) of the bovine PPAR-Ã?³gene with carcass and meat quality traits in 6.. Read More»

Genet. Mol. Res. 11(4):
2012.August.17.4
DOI:
10.4238/2012.August.17.4
Human Genetics   Research Article

Genetic analyses of the major and minor locus groups of bacterial wilt resistance in tobacco using a diallel cross design

Authors: Y.L. Qian1*, J. Chen2*, J.J. Dong3, Z.C. Wu4, Y.H. Liu3, B.Y. Xue3, F.W. Shao3 and X.Y. Sun1

Tobacco germplasm samples with various levels of resistance to bacterial wilt were selected to construct F1 combinations of parental inbred lines and orthogonal diallel crosses using samples collected in 2009 (15 germplasms), 2010 (15 germplasms), and 2011 (16 germplasms). A total of 1/2P (P + 1) experimental materials were used for analysis. Based on the.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017223
DOI:
10.4238/gmr.15017223
Human Genetics   Research Article

Decreased MEFV gene expression in rheumatoid arthritis patients

Authors: E.O. Etem, S.S. Koca, D. Erol, S. Yolbas, E. Oz, H. Elyas and A. Is�±k

Rheumatoid arthritis (RA) is a major cause of adult chronic inflammatory arthritis and an autoimmune disease of unknown etiology in which the inflammatory pathology involves T cell activation. Genetic mutations in the Mediterranean fever (MEFV) gene, encoding pyrin, influence the severity of RA, but the underlying mechanisms are not completely understood... Read More»

Genet. Mol. Res. 14(1):
2015.February.6.3
DOI:
10.4238/2015.February.6.3
Cytogenomics   Research Article

Mapping of AFLP loci linked to tolerance to cowpea golden mosaic virus

Authors: M.A. Rodrigues C.A.F. Santos J.R.F. Santana

AFLP markers combined with the bulk segregant analysis methodology was used for the identification of molecular markers associated with the cowpea golden mosaic virus (CGMV) resistance gene in 286 F2 cowpea plants derived from the cross IT97K-499-35 x Canapu T16. Segregation data in the F2 population demonstrated that tolerance to CGMV is controlled by a sin.. Read More»

Genet. Mol. Res. 11(4):
2012.August.17.12
DOI:
10.4238/2012.August.17.12
Animal Genetics   Research Article

Decoherence in yeast cell populations and its implications for genome-wide expression noise

Authors: M.R.S. Briones F. Bosco

Gene expression “noise” is commonly defined as the stochastic variation of gene expression levels in different cells of the same population under identical growth conditions. Here, we tested whether this “noise” is amplified with time, as a consequence of decoherence in global gene expression profiles (.. Read More»

Genet. Mol. Res. 8(1):
vol8-1gmr531
DOI:
10.4238/vol8-1gmr531
Animal Genetics   Research Article

Genetic characterization and evaluation of anthropogenic impacts on genetic patterns in cultured and wild populations of mussels (Mytilus galloprovincialis) from Greece

Authors: I.A. Giantsis N. Kravva A.P. Apostolidis

Despite the great commercial and economic importance of mussels, Mytilus galloprovincialis (Bivalvia, Mollusca) in Greece, little information is available concerning their population genetic structure. We used RAPD markers to examine genetic differentiation and potential impact of aquaculture practices and other anthropogenic activities on the genetic str.. Read More»

Genet. Mol. Res. 11(4):
2012.August.17.14
DOI:
10.4238/2012.August.17.14
Plant Genetics   Research Article

Global gene expression profile in myelodysplastic syndromes using SAGE

Authors: C.F. Mendiburu, W.A. Silva Jr., O. Ricci Jr., C.R. Bonini-Domingos and A.C. Fett-Conte

The molecular pathogenesis of myelodysplastic syndromes (MDS) is poorly understood. In order to expand our knowledge of genetic defects in MDS, we determined the overall profile of genes expressed in bone marrow from patients with refractory anemia with excess blasts (RAEB) by serial analysis of gene expression (SAGE). The present report describes a parti.. Read More»

Genet. Mol. Res. 7(4):
vol7-4gmr521
DOI:
10.4238/vol7-4gmr521
Human Genetics   Research Article

LEPR p.Q223R, �²3-AR p.W64R and LEP c.-2548G>A gene variants in obese Brazilian subjects

Authors: Duarte SFP, Francischetti EA, Genelhu VA, Cabello PH and Pimentel MMG

Obesity is due to the combined effects of genes, environment, lifestyle, and the interactions of these factors. The adrenergic receptor β3 (β3-AR), leptin (LEP) and leptin receptor (LEPR) genes have been intensively evaluated in the search of variants that could be related to obesity and its cardiometabolic complicat.. Read More»

Genet. Mol. Res. 6(4):