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Gene

Medical Genetics   Research Article

Drought-tolerant rice germplasm developed from an Oryza officinalis transformation-competent artificial chromosome clone

Authors: R. Liu, H.H. Zhang, Z.X. Chen, M.Q. Shahid, X.L. Fu and X.D. Liu

Oryza officinalis has proven to be a natural gene reservoir for the improvement of domesticated rice as it carries many desirable traits; however, the transfer of elite genes to cultivated rice by conventional hybridization has been a challenge for rice breeders. In this study, the conserved sequence of plant stress-related NAC transcription factors was s.. Read More»

Genet. Mol. Res. 14(4):
2015.October.28.29
DOI:
10.4238/2015.October.28.29
Animal Genetics   Research Article

SNP detection using RNA-sequences of candidate genes associated with puberty in cattle

Authors: S.J. Coleman, S.E. Speidel, R.M. Enns, A. Islas-Trejo, J.F. Medrano, S.S. Moore, M.R.S. Fortes, L.T. Nguyen, B. Venus, I.S.D.P. Diaz,F.R.P. Souza, L.F.S. Fonseca, F. Baldi, L.G. Albuquerque, M.G. Thomas, H.N. Oliveira

Fertility traits, such as heifer pregnancy, are economically important in cattle production systems, and are therefore, used in genetic selection programs. The aim of this study was to identify single nucleotide polymorphisms (SNPs) using RNA-sequencing (RNA-Seq) data from ovary, uterus, endometrium, pituitary gland, hypothala.. Read More»

Genet. Mol. Res. 16(1):
gmr16019522
DOI:
10.4238/gmr16019522
Bioinformatics   Research Article

Analysis of genetic diversity in Larix gmelinii (Pinaceae) with RAPD and ISSR markers

Authors: L. Zhang H.G. Zhang X.F. Li

Dahurian larch (Larix gmelinii), a deciduous conifer, is the northernmost tree, native to eastern Siberia and nearby regions of China. We used growth traits and molecular markers to assess genetic variation in different L. gmelinii growing regions; 105 individual samples were collected from seven regions of the Qingshan Forestry Centre, Heilongjiang Province.. Read More»

Genet. Mol. Res. 12(1):
2013.January.24.12
DOI:
10.4238/2013.January.24.12
Plant Genetics   Research Article

Effect of puerarin on the expression of NMU, NPY, and POMC genes in the hypothalamus

Authors: Q.D. Jiang1,2 and X.P. Li1

The hypothalamus is an important component of the nervous system, and neuropeptide Y (NPY), proopiomelanocortin (POMC), and neuromedin U (NMU) are key players in physiological regulation. Puerarin is important for nerve regulation. We investigated the effect of puerarin on the expression of NMU, NPY, and POMC genes in the hypothalamus. The results showed .. Read More»

Genet. Mol. Res. 15(1):
gmr.15017362
DOI:
10.4238/gmr.15017362
Plant Genetics   Research Article

Efficient isolation of high-quality RNA from lotus Nelumbo nucifera ssp nucifera tissues

Authors: Y.J. Zhang X.Y. Hao Z.S. Liang W.D. Ke H.B. Guo

Nelumbo nucifera is widely used as food, as an ornamental, in medicine, and as packing material; it is also reported to have anti-HIV effects and antioxidant capacity. We sought an improved method for extracting high-quality total RNA from different tissues of N. nucifera. Four methods for RNA extraction were assessed for their ability to recover high-qua.. Read More»

Genet. Mol. Res. 12(1):
2013.January.24.14
DOI:
10.4238/2013.January.24.14
Human Genetics   Research Article

Structure characterization of human cytomegalovirus UL131A, UL130 and UL128 genes in clinical strains in China

Authors: Z.-R. Sun, Y.-H. Ji, Q. Ruan, R. He, Y.-P. Ma, Y. Qi, Z.-Q. Mao,Y.-J. Huang

Human cytomegalovirus (HCMV) genetic determinants of endothelial cell tropism, leukocytes and dendritic cells have been identified in the genes UL131A, UL130, and UL128. We examined the structure of these three genes in HCMV. Eighteen low-passage clinical isolates and five non-passage strains from congenitally HCMV-infected in.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr654
DOI:
10.4238/vol8-3gmr654
Human Genetics   Research Article

Genetic variability and heterogeneity of Venezuelan equine encephalitis virus vector Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations of the Colombian Atlantic coast, based on microsatellite loci

Authors: F. Bello and V. Becerra

In Colombia, the mosquito Ochlerotatus taeniorhyn­chus has been identified as an efficient vector of the epidemic-epi­zootic Venezuelan equine encephalitis virus. We evaluated the ge­netic variability and heterogeneity of this mosquito in Colombian populations using eight microsatellite DNA loci. Two hundred and te.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr652
DOI:
10.4238/vol8-3gmr652
Human Genetics   Research Article

Relationship between CYP17 gene polymorphisms and risk of prostate cancer

Authors: J. Song, Z.-H. Tao, X.-Y. Liu, S. Gong and L. Gan

Cytochrome P450 17a-hydroxylase (CYP17) plays a critical role in androgen biosynthesis. Polymorphisms of the CYP17 promoter have been proposed as risk factors for prostate cancer; however, some studies have produced inconclusive or controversial results. We investigated the relationship between polymorphisms of the CYP17 gene and the risk of prostate canc.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017866
DOI:
10.4238/gmr.15017866
Human Genetics   Research Article

Correlation between the development of calcium oxalate stones and polymorphisms in the fibronectin gene in the Uighur population of the Xinjiang region of China

Authors: M. Murat, A. Aekeper, L.Y. Yuan, T. Alim, G.J. Du, A. Abdusamat,G.W. Wu and Y. Aniwer

Here, we have investigated the correlation between calcium oxalate stone formation and Fn gene polymorphisms in urinary calculi patients among the Uighur population (Xinjiang region). In this case control study, genomic DNA extracted from the peripheral blood of 129 patients with calcium oxalate stones (patient group) and 94 normal people (control group) .. Read More»

Genet. Mol. Res. 14(4):
2015.October.28.35
DOI:
10.4238/2015.October.28.35
Cytogenomics   Research Article

Genetic diversity analysis in Opal cotton hybrids based on SSR, ISSR, and RAPD markers

Authors: Z. Noormohammadi Y. Hasheminejad-Ahangarani Farahani M. Sheidai S. Ghasemzadeh-Baraki O. Alishah

Cotton is one of the most economically important crops in Iran; hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. We examined agronomic characteristics and molecular genetic diversity in the Opal cotton (Gossypium hirsutum) cultivar and in F2 progenies. Ten homo-primers and seven hetero-primers of 26 RAPD.. Read More»

Genet. Mol. Res. 12(1):
2013.January.30.12
DOI:
10.4238/2013.January.30.12