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Gene

Plant Genetics   Research Article

Comparison of coefficients and distance measurements in passion fruit plants based on molecular markers and physicochemical descriptors

Authors: C.B.M. Cerqueira-Silva1, C.B. Cardoso-Silva2, L.D.H.C.S. Concei�§�£o3, J.V.A. Nonato2, A.C. Oliveira2 and R.X. Corr�ªa3

We investigated seven distance measures and 14 simi­larity coefficients in a set of observations of variables of the ‘yellow’ passion fruit plant (Passiflora edulis Sims), submitted to multivari­ate analyses (distance, projection and grouping). Fourteen genotypes were characterized, based on DNA amplification with 16 random am­plif.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr614
DOI:
10.4238/vol8-3gmr614
Human Genetics   Research Article

A novel NF1 mutation in a Chinese patient with giant caf�©-au-lait macule in neurofibromatosis type 1 associated with a malignant peripheral nerve sheath tumor and bone abnormality

Authors: H.-X. Tong M. Li Y. Zhang J. Zhu W.-Q. Lu

Neurofibromatosis type 1 (NF1; OMIM#162200) is a common neurocutaneous disorder that is characterized by multiple café-au-lait, skinfold freckling, Lisch nodules, and neurofibromas. Mutations in the NF1 gene, which encodes the neurofibromin protein, have been identified as the pathogenic gene of NF1. In this study, we present a clinical and molecul.. Read More»

Genet. Mol. Res. 11(3):
2012.July.10.6
DOI:
10.4238/2012.July.10.6
Human Genetics   Research Article

Generation of a preliminary bovine gene atlas, using expression clustering to annotate gene function

Authors: O.M. Keane1,3, N. Maqbool2, A.F. McCulloch2, J.C. McEwan2 and K.G. Dodds2

Genes whose products function in a common biological process are often co-regulated. When regulation occurs at the transcriptional level, co-expressed genes can be detected globally by expression arrays or by sequencing non-normalized cDNA libraries. We examined bovine gene expression in 27 tissues using non-normalized cDNA library sequencing. Contigs wer.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr594
DOI:
10.4238/vol8-3gmr594
Human Genetics   Research Article

Gene expressions levels of 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPase�² in gill tissue of Mugil cephalus acclimated to low salinity

Authors: L. Li, M. Jiang and X.Q. Shen

Fishes adapt to salinity changes primarily through osmotic pressure regulation, a process often associated with several genes, including 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPaseβ. The present study investigated the differential expression of genes 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPaseβ in the gill tissue of Mu.. Read More»

Genet. Mol. Res. 16(1):
gmr16019444
DOI:
10.4238/gmr16019444
Animal Genetics   Research Article

PIT1 gene polymorphism in Pietrain and Large White pigs after divergent selection

Authors: A.C.P. Silveira1, T.F. Braga1, J.F. Almeida2, R.C. Antunes1,P.F.A. Freitas1, A.S.M. Cesar1 and E.C. Guimar�£es1

We examined the polymorphisms in the PIT1 gene of 103 pigs and compared their frequencies in the maternal and paternal lineages of the Pietrain and Large White breeds, which have undergone divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by PCR and genotyped by RFLP, using the restriction enzyme R.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr609
DOI:
10.4238/vol8-3gmr609
Human Genetics   Research Article

Osteoblast differentiation of rabbit adipose-derived stem cells by polyethylenimine-mediated BMP-2 gene transfection in vitro

Authors: Q.F. Wang, Y. Huang, G.C. He, H.S. Wang, Z.H. Chen, X.H. Cai, Y.H. Xie and Q. Liu

The aim of this study was to examine the efficiency of polyethylenimine-mediated transfection of the human bone morphogenetic protein-2 (BMP-2) gene into rabbit adipose-derived stem cells (ADSCs), and its effect on osteoblast differentiation. Adipose tissue was isolated from the necks of adult Japanese white rabbits and cultur.. Read More»

Genet. Mol. Res. 16(1):
gmr16015358
DOI:
10.4238/gmr16015358
Human Genetics   Research Article

Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene

Authors: R.T. Maia1, V.M. Scarpassa2, L.H. Maciel-Litaiff3 and W.P. Tadei2

Aedes albopictus, a mosquito originally from Southeast Asia, is considered to be one of the main vectors of dengue fever, yel­low fever and other arboviruses. We examined the genetic variability and population structure of 68 individuals of Ae. albopictus collected from five neighborhoods of the city of Manaus, based on the mitochon­drial gene cod.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr624
DOI:
10.4238/vol8-3gmr624
Plant Genetics   Research Article

Leaf carbon assimilation and molecular phylogeny in Cattleya species (Orchidaceae)

Authors: V. Andrade-Souza, A.-A.F. Almeida, R.X. Corr�ªa, M.A. Costa,\M.S. Mielke and F.P. Gomes

We examined leaf CO2 assimilation and how it varied among species within the orchid genus Cattleya. Measurements of CO2 assimilation and maximum quantum yield of PS II (Fv/Fm) were made for mature leaves of nine species using a portable system for photosyn­thesis measurement and a portable fluorometer. Leaf area was measured with an area meter, and th.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr618
DOI:
10.4238/vol8-3gmr618
Animal Genetics   Research Article

Identification of the e allele at the Extension locus (MC1R) in Brazilian Creole sheep and its role in wool color variation

Authors: D. Hepp G.L. Gon�§alves G.R.P. Moreira T.R.O. Freitas C.T.D.C. Martins T.A. Weimer D.T. Passos

The melanocortin 1 receptor (MC1R) gene has been described as responsible for the black color in some breeds of sheep, but little is known about its function in many colored breeds, particularly those with a wide range of pigmentation phenotypes. The Brazilian Creole is a local breed of sheep from southern Brazil that has a wide variety of wool colors. We.. Read More»

Genet. Mol. Res. 11(3):
2012.May.22.5
DOI:
10.4238/2012.May.22.5
Plant Genetics   Research Article

In vitro regeneration of cocona (Solanum sessiliflorum, Solanaceae) cultivars for commercial production

Authors: A.R. Schuelter1, A.K. Grunvald1, A.T. Amaral J�ºnior2, C.L. da Luz1,C.L. Luz1, L.M. Gon�§alves1, S. Stefanello1 and C.A. Scapim3

Cocona (Solanum sessiliflorum Dunal) is a solanaceous shrub native to the Amazon region that produces an edible fruit. This species has numerous advantages, particularly a high nutritional value and productivity. However, due to irregular germination and rapid loss of seed viability, there are few plantations for production on a large scale. Development o.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr613
DOI:
10.4238/vol8-3gmr613