Gene
Cloning of superoxide dismutase from post-harvest Hami melon and quantitative expression analysis before and after disease
Authors: C.H. Shan, F.X. Tang, W. Chen and W.R. Ma
Primers were designed according to the Cu/Zn-SOD gene sequences of cloned Cucurbits plants (cucumbers and watermelons) available in NCBI. Total RNA from Hami melon pulp was used as a template. Following RT-PCR amplification, a 403-bp fragment of the Hami melon Cu/Zn-SOD gene was obtained. According to alignment in BLAST and phylogenetic tree analysis, the.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.10
- DOI:
- 10.4238/2015.December.23.10
Genetic diversity and structure in natural populations of Maytenus truncata Reiss, 1861, a medicinal plant vulnerable to extractivism in Bahia State, Brazil
Authors: R.R. Simplicio, A.M. Waldschmidt, M.B. Amorim, B.S. Almeida and D.G. Pereira
Maytenus truncata (Celastraceae) is a plant species widely used in the treatment of ulcers and tumors. Despite the intensive harvest of native specimens in the State of Bahia, northeastern Brazil, there is no information about the genetic variability or structure of this species. Therefore, the goal of this study was to estimate the genetic diversity and .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.11
- DOI:
- 10.4238/2015.December.23.11
Phylogenetic relationships of Malayan gaur with other species of the genus Bos based on cytochrome b gene DNA sequences
Authors: M.K.A. Rosli, S.S. Zakaria, S.M.F. Syed-Shabthar, Z.Z. Zainal, M.N. Shukor, M.C. Mahani, O. Abas-Mazni and B.M. Md-Zain
The Malayan gaur (Bos gaurus hubbacki) is one of the three subspecies of gaurs that can be found in Malaysia. We examined the phylogenetic relationships of this subspecies with other species of the genus Bos (B. javanicus, B. indicus, B. taurus, and B. grunniens). The sequence of a key gene, cytochrome b, was compared among 20.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1002
- DOI:
- 10.4238/vol10-1gmr1002
Determination of genetic variation among chickpea genotypes and their F1 crosses with RAPD markers
Authors: K. Mahmood, W. Nazeer, M. Naeem, S. Freed, M.A. Khan, G. Abbas, M. Iqbal, A.R. Kulachi, S.A. Sajid, M.A. Sadiq and M. Saleem
The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F1 crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among 6 chickpea genotypes and 15 F1 crosses with the RAPD markers. The 6 parents and.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.1
- DOI:
- 10.4238/2013.April.10.1
Study of the methylation patterns of the EGFR gene promoter in non-small cell lung cancer
Authors: Z.Y. Pan, Z.S. Jiang and H.Q. Ouyang
We investigated the methylation state of the epidermal growth factor receptor (EGFR) gene promoter in non-small cell lung cancer (NSCLC) and analyzed its effect on tumor biology. We enrolled 120 patients with NSCLC who had been confirmed by pathologic diagnosis and had been operated on. The methylation states of the EGFR gene promoter were detected and an.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.14
- DOI:
- 10.4238/2015.August.19.14
Association between the XRCC1 Arg399Gln polymorphism and risk of cervical carcinoma: a meta-analysis
Authors: D.Y. Liu, H.C. Liang and X.M. Xiao
Numerous studies have evaluated the association between Arg399Gln polymorphism of DNA repair gene XRCC1 and cervical carcinoma risk. However, the specific association is still controversial. To assess the relationship between XRCC1 Arg399Gln polymorphism and cervical carcinoma, we conducted a comprehensive meta-analysis of 10 case-control studies with 205.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.15
- DOI:
- 10.4238/2015.August.19.15
De novo assembly, functional annotation, and marker development of Asian pear (Pyrus pyrifolia) fruit transcriptome through massively parallel sequencing
Authors: J.F. Li, Z. Gao, Y.S. Lou, M. Luo, S.R. Song, W.P. Xu, S.P. Wang and C.X. Zhang
This study investigated the Asian pear transcriptome using the RNA-Seq normalized fruit cDNA library to create a transcriptomic resource for unigene and marker discovery. Following the removal of lowquality reads, 127,085,054 trimmed reads were assembled de novo to yield 37,649 non-redundant unigenes with an average length of 599 bp. Alternative splicing .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.22
- DOI:
- 10.4238/2015.December.23.22
Study of the genetic diversity and structure of a natural population of Nectandra megapotamica (Spreng.) Mez. using RAPD markers
Authors: L.S. Costa, L.R.S. Reiniger, B.M. Heinzmann, L.P. Amaral and C.M.L. Serrote
Nectandra megapotamica (Spreng.) Mez. is a tree species that naturally occurs in the Atlantic Forest, Brazil. The aim of this study was to evaluate the genetic diversity and structure of a natural population of 12 N. megapotamica individuals using random amplified polymorphic DNA markers. Eleven primers were used in this study, producing 81 bands, of whic.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.28
- DOI:
- 10.4238/2015.December.23.28
Genetic relatedness of artichoke (Cynara scolymus L.) hybrids using random amplified polymorphic DNA (RAPD) fingerprinting
Authors: M.A. Sharaf-Eldin, A. Al-Tamimi, P. Alam, S.F. Elkholy and J.R. Jordan
The artichoke (Cynara scolymus L.) is an important food and medicinal crop that is cultivated in Mediterranean countries. Morphological characteristics, such as head shape and diameter, leaf shape, and bract shape, are mainly affected by environmental conditions. A molecular marker approach was used to analyze the degree of polymorphism between artichoke .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.31
- DOI:
- 10.4238/2015.December.23.31
Selection of reference genes for expression analyses of red-fleshed sweet orange (Citrus sinensis)
Authors: T.T. Pinheiro, D.S. Nishimura, F.B. De Nadai, A. Figueira and R.R. Latado
Red-fleshed oranges (Citrus sinensis) contain high levels of carotenoids and lycopene. The growing consumer demand for products with health benefits has increased interest in these types of Citrus cultivars as a potential source of nutraceuticals. However, little is known about the physiology of these cultivars under Brazilian conditions. Transcriptome an.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.32
- DOI:
- 10.4238/2015.December.23.32