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Gene

Cytogenomics   Research Article

Whole-genome duplications contributed to the expansion of cytochrome b5 genes in Paramecium tetraurelia

Authors: K.J. Wu, D. Wang, J. Ding, S.H. Yang and X.H. Zhang

Cytochrome b5 (cyt b5) genes encode ubiquitous electron transport hemoproteins found in animals, plants, fungi, and purple bacteria. However, little is known about their evolutionary history in genomes so far. Here, we conducted an extensive genome-wide survey of cyt b5 genes in 20 representative model species and identified 310 of these genes. Both the a.. Read More»

Genet. Mol. Res. 12(2):
2013.January.9.1
DOI:
10.4238/2013.January.9.1
Microbial Genetics   Research Article

Cloning and polymorphism analysis of the 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase gene (VTE3) in Arachis hypogaea, A. duranensis, and A. ipa�«nsis

Authors: F.Z. Liu, A.Q. Guo and Y.S. Wan

One of the critical enzymes involved in vitamin E biosynthesis in plants is 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase (MPBQ MT). The full-length VTE3 cDNA (designated rVTE3-1 and -2) encoding MPBQ MT and the full-length DNA of VTE3 (designated gVTE3-1 and -2) were isolated from cultivated peanuts (Arachis hypogaea). The full-length DNA of VTE3 (.. Read More»

Genet. Mol. Res. 12(2):
2013.January.4.5
DOI:
10.4238/2013.January.4.5
Plant Genetics   Research Article

Mating system patterns of natural populations of Pinus koraiensis along its post-glacial colonization route in northeastern China

Authors: X. Sui,F.J. Feng,D. Zhao,M. Xing,X.Y. Sun,S.J. Han and M.H. Li

To understand the genetic mechanisms underlying the endangerment of Pinus koraiensis, we studied the mating system of 49 families of this species in 3 natural populations along its post-glacial colonization route across ~1500 km in northeastern China using the chloroplast simple sequence repeat technique. We analyzed 11 polymorphic loci with clear and rep.. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.27.26
DOI:
http://dx.doi.org/10.4238/2015.April.27.26
Microbial Genetics   Research Article

Highly specific detection of genetic modification events using an enzyme-linked probe hybridization chip

Authors: M.Z. Zhang, X.F. Zhang, X.M. Chen, X. Chen, S. Wu and L.L. Xu

The enzyme-linked probe hybridization chip utilizes a method based on ligase-hybridizing probe chip technology, with the principle of using thio-primers for protection against enzyme digestion, and using lambda DNA exonuclease to cut multiple PCR products obtained from the sample being tested into single-strand chains for hybridization. The 5'-end amino-l.. Read More»

Genet. Mol. Res. 14(3):
2015.August.10.10
DOI:
10.4238/2015.August.10.10
Human Genetics   Research Article

Association between polymorphisms in the adiponectin gene (APM-1) and atherosclerotic cerebral infarction in a Hainan Chinese Han population

Authors: P. He, X.L.Chen and Y.P. Ding

We investigated the association between polymorphisms in the adiponectin gene (APM-1) and atherosclerotic cerebral infarction (ACI) in a Chinese Han population of Hainan Province. Polymerase chain reaction-restriction fragment length polymorphism and gene sequencing were used to analyze the distribution of APM-1 +45T/G and +276G/T genotypes and their alle.. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.27.29
DOI:
http://dx.doi.org/10.4238/2015.April.27.29
Microbial Genetics   Research Article

Ectopic expression of the BoTFL1-like gene of Bambusa oldhamii delays blossoming in Arabidopsis thaliana and rescues the tfl1 mutant phenotype

Authors: H.Y. Zeng, Y.T. Lu, X.M. Yang, Y.H. Xu and X.C. Lin

TERMINAL FLOWER1 (TFL1) homologous genes play major roles in maintaining vegetative growth and inflorescence meristem characteristics in various plant species; however, to date, the function of the bamboo TFL1 homologous gene has not been described. In this study, a TFL1 homologous gene was isolated from Bambusa oldhamii and designated as BoTFL1-like. Phy.. Read More»

Genet. Mol. Res. 14(3):
2015.August.10.11
DOI:
10.4238/2015.August.10.11
Human Genetics   Research Article

Novel exon nucleotide deletion causes adrenoleukodystrophy in a Brazilian family

Authors: E.R. Valadares*, A.L.C. Trindade*, L.R. Oliveira, R.R. Arantes, M.V. Daker, B.M. Viana, V.G. Haase, L.B. Jardim, G.C. Lopes and A.L.B. Godard

Adrenoleukodystrophy is a neurodegenerative X-linked recessive disorder. It is characterized by abnormal function of peroxisomes, which leads to an accumulation of very long-chain fatty acids in plasma and tissues, especially in the cortex of adrenal glands and white matter of the central nervous system, causing demyelinating .. Read More»

Genet. Mol. Res. 10(1):
vol10-1gmr975
DOI:
10.4238/vol10-1gmr975
Animal Genetics   Research Article

MicroRNA-21 promotes proliferation of rat hepatocyte BRL-3A by targeting FASLG

Authors: J.J. Li,W.H. Chan,W.Y. Leung,Y. Wang and C.S. Xu

Rat liver regeneration (RLR) induced by partial hepatectomy involves cell proliferation regulated by numerous factors, including microRNAs (miRNAs). miRNA high-throughput sequencing has been established and used to analyze miRNA expression profiles. This study showed that 39 miRNAs were related to RLR through the analysis of miRNA high-throughput sequenci.. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.27.30
DOI:
http://dx.doi.org/10.4238/2015.April.27.30
Medical Genetics   Research Article

Investigation of polymorphisms in exon7 of the NSUN7 gene among Chinese Han men with asthenospermia

Authors: H.Y. Ren, R. Zhong, X.P. Ding, Z.Y. Chen and Y.L. Jing

Previous studies have shown that genetic polymorphisms in exon7 of the NSUN7 gene can be used as an infertility marker in Iranian men with asthenospermia. However, there have been no equivalent studies in China. In the present study, we investigated the possible association between the genetic polymorphisms in exon7 of NSUN7 and asthenospermia in a Chines.. Read More»

Genet. Mol. Res. 14(3):
2015.August.10.6
DOI:
10.4238/2015.August.10.6
Microbial Genetics   Research Article

Cloning and transformation analysis of isoflavone synthase gene into Minshan Trifolium pratense

Authors: H.H. Hu, C.Q. Jing, R. Liu, W.D. Li and H.G. Feng

The aim of this study was to clone the isoflavone synthase (IFS) gene and establish the recombinant Minshan Trifolium pratense. The IFS gene was cloned from the callus of Minshan T. pratense using reverse transcription-polymerase chain reaction. The plant expression vector pRI101-AN-IFS was constructed and introduced into Agrobacterium tumefaciens strain .. Read More»

Genet. Mol. Res. 14(3):
2015.August.10.9
DOI:
10.4238/2015.August.10.9