Gene
Isolation and characterization of microsatellite markers for Bertholletia excelsa (Lecythidaceae) population genetic analysis
Authors: P.S. Sujii1, P.W. Inglis2, A.Y. Ciampi2, V.N. Solferini1 and V.C.R. Azevedo2
Seven polymorphic microsatellite markers were developed and validated for Bertholletia excelsa (Brazil nut tree) population genetic studies. This species is a widespread monotypic Amazonian tree with high non-timber economic value. Unfortunately, Brazil nut production is currently less than 25% of historical production levels, because of extensive defores.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.November.7.2
- DOI:
- 10.4238/2013.November.7.2
Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus
Authors: L.N. Sun1,2, H.S. Yang1, M.Y. Chen3 and D.X. Xu1,2
Many developmental genes play important roles in regeneration. Here, we cloned the two developmental genes, Wnt6 and Hox6, from Apostichopus japonicus by rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The Wnt6 full-length cDNA is 1140 bp long with a 1029-bp open reading frame encoding 342 amino acids. The deduced Wnt6 protein con.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.7
- DOI:
- 10.4238/2013.November.7.7
Genetic variability of Dactylopius opuntiae (Hemiptera, Dactylopiidae) on forage cactus in northeast Brazil
Authors: D.M.P. Silva1, A.C. do E.S. Mergulh�£o2, L.V. de Medeiros2,M.V.B. Figueiredo3 and H.A. Burity3
The carmine cochineal Dactylopius opuntiae is a key pest in productive fields of forage cactus in Pernambuco, Brazil. Species identification by means of molecular markers assists in understanding the genetic profile, underpins morphological characterization, and supports the monitoring of populations in integrated management programs designed to control t.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.8
- DOI:
- 10.4238/2013.October.30.8
Molecular analysis of East Anatolian traditional plum and cherry accessions using SSR markers
Authors: M.H. ��z1, H. Vurgun1, M. Bakir2, �°. B�¼y�¼k3, C. Y�¼ksel2, H.M. ��nl�¼1, K. ��ukadar1, B. Karado��an1, ��. K�¶se3 and A. Erg�¼l2
We conducted SSR analyses of 59 accessions, including 29 traditional plum (Prunus domestica), 24 sweet cherry (Prunus avium), and 1 sour cherry (Prunus cerasus) selected from East Anatolian gene sources and 3 plum and 2 cherry reference accessions for molecular characterization and investigation of genetic relationships. Eight SSR loci [1 developed from t.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.6
- DOI:
- 10.4238/2013.November.7.6
Cloning, sequencing, and polymorphisms of the Wistar-Imamichi rat growth hormone gene using PCR-SSCP
Authors: M.Q. Liu1, J.G. Wang2* and X.L. Li2*
We successfully cloned the Wistar-Imamichi (WI) rat growth hormone gene (GenBank accession: GQ890681), which contained 5 exons and 4 introns. Using the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) technique, a novel missense substitution single nucleotide polymorphism was identified and tested for Hardy-Weinberg equilibrium.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.December.4.7
- DOI:
- 10.4238/2013.December.4.7
High genetic variation among Aschersonia placenta (Clavicipitaceae) isolates from citrus orchards in China
Authors: P.P. Wang1,2, X.H. Song1,3 and H.Y. Zhang
Aschersonia placenta had been recognized as an important fungal pathogen of whiteflies. In recent years, natural occurrence of Aschersonia in whitefly populations was observed in many citrus orchards in the southern regions of China. We analyzed 60 A. placenta isolates obtained from Chinese citrus orchards, using inter-simple sequence repeats to examine t.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.December.4.6
- DOI:
- 10.4238/2013.December.4.6
Wilms' tumor suppressor gene mutations in girls with sporadic isolated steroid-resistant nephrotic syndrome
Authors: Y.H. Yang1*, F. Zhao1*, D.N. Feng1, J.J. Wang1, C.F. Wang1, J. Huang1, X.J. Nie1, G.Z. Xia1, G.M. Chen1 and Z.H. Yu1,2,3
Mutations in the Wilms' tumor suppressor gene (WT1) can lead to syndromic forms of steroid-resistant nephrotic syndrome (SRNS) such as Denys-Drash or Frasier syndrome and can cause isolated SRNS. A mutation within WT1 is a frequent cause of sporadic isolated SRNS in girls. In a worldwide cohort of girls, the rate of occurrence.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.December.4.5
- DOI:
- 10.4238/2013.December.4.5
Genetic diversity of the Arctic fox using SRAP markers
Authors: M. Zhang1 and X.J. Bai2
Sequence-related amplified polymorphism (SRAP) is a recently developed molecular marker technique that is stable, simple, reliable, and achieves moderate to high numbers of codominant markers. This study is the first to apply SRAP markers in a mammal, namely the Arctic fox. In order to investigate the genetic diversity of the .. Read More»
- Genet. Mol. Res. 13(4):
- 2013.December.4.4
- DOI:
- 10.4238/2013.December.4.4
Expression of calpastatin and myostatin genes associated with lamb meat quality
Authors: A. Bagatoli1, E. Gasparino1, M.A.M. Soares2, R.M. Amaral1,F.A.F. Macedo1, D.M. Voltolini1 and A.P. Del Vesco1
Calpastatin (CAST) is an endogenous calpain inhibitor and its main function is to modulate the proteolytic action of enzymes responsible for post-mortem myofibril deterioration. The myostatin gene (GDF-8) acts as a negative regulator of skeletal muscle growth. The expression of these two genes, as well as their interaction, affects the quality of the meat.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.December.4.3
- DOI:
- 10.4238/2013.December.4.3
Cross-amplification of nonspecific micro-satellites markers: a useful tool to study endangered/vulnerable species of southern Andes deer
Authors: J.C. MarÃ?Ân, P. Orozco-terWengel, K. Romero, J.P. VÃ?¡squez, V. Varas and J.A. Vianna
Thirty-nine microsatellite loci that are highly conserved in red deer, sika deer, reindeer, Soay sheep, and other artiodactyls were tested in two vulnerable and endangered Neotropical deer (pudu: Pudu puda and huemul: Hippocamelus bisulcus) with the aim of producing a standardized set of markers that can be used successfully i.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.25.4
- DOI:
- 10.4238/2014.April.25.4