Gene
Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers
Authors: E.T. Werner, T.C.B. Soares, A.B.P.L. Gontijo, J.D. Souza Neto and J.A.T. do Amaral
Crambe (Crambe abyssinica) is a non-edible annual herb, which was first cultivated to extract oil for industry, and now has great potential for biodiesel production. The objective of this investigation was to evaluate the genetic stability of micropropagated plants of the C. abyssinica Hochst cultivar ‘FMS brilhante’ using polymerase chain rea.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.
- DOI:
- 10.4238/2015.
Prospecting candidate SNPs for backfat in Canchim beef cattle
Authors: G.B. Veneroni, S.L. Meirelles, D.A. Grossi, G. Gasparin, A.M.G. Ibelli, P.C. Tizioto, H.N. Oliveira, M.M. Alencar and L.C.A. Regitano
Canchim is a composite cattle breed developed in Brazil for beef production. One of the breeding objectives is to increase fat deposition. QTLs for fat thickness and/or marbling have been reported on BTA4 and BTA14. The IGFBP3 and DDEF1 genes, mapped to BTA4 and BTA14, respectively, affect adipogenesis. We looked for SNPs in t.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr788
- DOI:
- 10.4238/vol9-4gmr788
Analysis of glutathione peroxidase 1 gene polymorphism and Keshan disease in Heilongjiang Province, China
Authors: H.L. Wei, J.R. Pei, C.X. Jiang, L.W. Zhou, T. Lan, M. Liu and T. Wang
Keshan disease (KD) is an endemic cardiomyopathy associated with selenium deficiency. Recent studies indicate that glutathione peroxidase 1 (GPx1) mutation decreases GPx activity in myocardial cells and increases the risk of KD. To further clarify the correlation between GPx1 polymorphism and KD, we analyzed GPx1 polymorphism,.. Read More»
- Genet. Mol. Res. 10(4):
Isolation of fertility-related genes of multiple-allele-inherited male sterility in Brassica rapa ssp pekinensis by cDNA-AFLP
Authors: R.Q. Ji, Q. Song, X.F. Xin, X. Zhou and H. Feng
To better understand the molecular mechanisms of multiple-allele-inherited male sterility in Chinese cabbage (Brassica rapa ssp pekinensis), differentially expressed genes in fertile and sterile plants must be isolated. We used cDNA-AFLP analysis to isolate differentially expressed genes in fertile and sterile buds of the two-.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.5.8
- DOI:
- 10.4238/2011.December.5.8
Genetic relationships between selected Turkish mulberry genotypes (Morus spp) based on RAPD markers
Authors: E. Orhan and S. Ercisli
Mulberry (Morus spp, Moraceae) is an important horticultural crop in Turkey, which is one of the main world producers of mulberry fruit. We evaluated the genetic relationships among 26 mulberry genotypes selected for agronomic characteristics, using RAPD markers. A total of 367 DNA markers were generated with 34 random primers.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr958
- DOI:
- 10.4238/vol9-4gmr958
Evidence for an association between haptoglobin and MnSOD (Val9Ala) gene polymorphisms in essential hypertension based on a Brazilian case-control study
Authors: A.L. Miranda-Vilela, A.K. Akimoto, P.C.Z. Alves, L.B. Ferreira, G.S. Lordelo, J.G.M. Melo, C.K. Grisolia, S.F. Oliveira and M.N. Klautau-Guimar�£es
Essential hypertension is a complex and multifactorial trait; genetic and environmental factors interact to produce the final phenotype. Studies have demonstrated association of hypertension with varied gene polymorphisms. However, demonstration of common genetic causes in the general population remains elusive. We investigate.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr973
- DOI:
- 10.4238/vol9-4gmr973
Simple method for culture of peripheral blood lymphocytes of Testudinidae
Authors: T.L. Silva, M.I.A. Silva, L.P.R. Venancio, C.E.S. Zago, V.A.G. Moscheta, A.V.B. Lima, L.D. Vizotto, J.R. Santos, C.R. Bonini-Domingos and M.T.V. Azeredo-Oliveira
We developed and optimized a simple, efficient and inexpensive method for in vitro culture of peripheral blood lymphocytes from the Brazilian tortoise Chelonoidis carbonaria (Testudinidae), testing various parameters, including culture medium, mitogen concentration, mitotic index, culture volume, incubation time, and mitotic a.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.6.2
- DOI:
- 10.4238/2011.December.6.2
Improvements in cytological preparations for fluorescent in situ hybridization in Passiflora
Authors: M.M. Souza, J.D. Urdampilleta and E.R. Forni-Martins
Cytological preparations for the fluorescent in situ hybridization (FISH) technique require cytoplasm-free metaphases, with well-spread chromosomes, for the localization of DNA sequences and chromosome mapping. We tested various procedures for FISH analysis of Passiflora cacaoensis, P. gardneri and hybrid F1 progeny of P. gard.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr951
- DOI:
- 10.4238/vol9-4gmr951
Diversity of platelet function and genetic polymorphism in clopidogrel-treated Chinese patients
Authors: B. Sun, J. Li, M. Dong, L. Yang, C. Wu, L. Zhu and Y.L. Cong
We investigated the correlation between genetic polymorphisms of cytochrome P450 enzyme genes and the outcome of clopidogrel treatment in 118 coronary disease patients after percutaneous coronary intervention at the Chinese PLA General Hospital. Patients were divided into an ischemia event relapse group (IERG) and a non-IERG group (NIERG) based on relapse.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.13.22
- DOI:
- 10.4238/2015.February.13.22
Lack of association of genetic polymorphisms of angiotensin-converting enzyme gene I/D and glutathione-S-transferase enzyme T1 and M1 with retinopathy of prematures
Authors: M. Yildiz, M. Karkucak, T. Yakut, O. Gorukmez and A. Ozmen
One of the most frequently observed causes of blindness in infancy is the pathogenesis known as retinopathy of prematurity (ROP). Angiotensin-converting enzyme (ACE) is a vital enzyme in the renin-angiotensin-aldosterone system; it is involved in the development of cardiovascular system diseases linked to I/D polymorphism of t.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr887
- DOI:
- 10.4238/vol9-4gmr887