Gene
Isolation and characterization of the papaya MADS-box E-class genes, CpMADS1 and CpMADS3, and a TM6 lineage gene CpMADS2
Authors: M.-J. Lee, W.-J. Yang, C.-T. Chiu, J.-J. Chen, F.-C. Chen and L.-S. Chang
Papaya (Carica papaya L.) plants are polygamous, with female, male, and hermaphroditic flowers. To understand the roles of MADS-box genes in flower development and sex determination, we cloned cDNAs of E-class genes CpMADS1 and CpMADS3 and a TM6 lineage of the B-class gene CpMADS2 from young.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.9
- DOI:
- 10.4238/2014.July.24.9
Evidence of balancing selection in multiple indigenous chicken populations
Authors: S. Arlud, G.X. E and S.C. Zeng
Microsatellite DNA markers, which are assumed to drift, have been widely used to assess genetic diversity in all major domestic animal species. These markers provide insight into the arrival and dispersion history of a species, with regard to their content or management history. However, no direct evidence supports current standard microsatellite markers .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038249
- DOI:
- 10.4238/gmr.15038249
Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium �Earsakul� mutants
Authors: A. Wannajindaporn, O. Poolsawat, W. Chaowiset and P.A. Tantasawat
In vitro mutagenesis of Dendrobium ‘Earsakul’ was carried out by incubating the protocorm-like bodies in 0-5 mM sodium azide for 1 h. Twenty-eight putative mutants were evaluated for genetic variability compared to untreated control plants using inter-simple sequence repeat (ISSR) analysis. Polymo.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.12
- DOI:
- 10.4238/2014.July.24.12
Salicornia europaea L. Na+/H+ antiporter gene improves salt tolerance in transgenic alfalfa (Medicago sativa L.)
Authors: L.Q. Zhang, Y.D. Niu*, H. Huridu, J.F. Hao, Z. Qi and A. Hasi
In order to obtain a salt-tolerant perennial alfalfa (Medicago sativa L.), we transferred the halophyte Salicornia europaea L. Na+/H+ antiporter gene, SeNHX1, to alfalfa by using the Agrobacterium-mediated transformation method. The transformants were confirmed by both PCR and RT-PCR analyse.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.14
- DOI:
- 10.4238/2014.July.24.14
Genetic variation in and spatial structure of natural populations of Dipterocarpus alatus (Dipterocarpaceae) determined using single sequence repeat markers
Authors: N.M. Tam, V.D. Duy, N.M. Duc, V.D. Giap and B.T.T. Xuan
Dipterocarpus alatus (Dipterocarpaceae) is widely distributed in lowland forests in central and southern Vietnam, Cambodia, Laos, Myanmar, Philippines, Thailand, and India. Due to over-exploitation and habitat destruction, the species is now threatened. The genetic variation within and among populations of D. alat.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.17
- DOI:
- 10.4238/2014.July.24.17
A novel NF1 frame-shift mutation (c.702_703delGT) in a Chinese family with neurofibromatosis type 1
Authors: S.P. Cai, N. Fan, J. Chen, Z.L. Xia, Y. Wang, X.M. Zhou, Y. Yin, T.L. Wen, Q.J. Xia, X.Y. Liu and H.Y. Wang
This study aimed to characterize the clinical features of a Chinese pedigree with neurofibromatosis type 1 (NF1) and to identify mutations in the NF1 gene. In this three-generation family containing 8 members, 5 had been diagnosed with NF1 and the others were asymptomatic. All members of the family underwent complete medical e.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.19
- DOI:
- 10.4238/2014.July.24.19
Cloning and characterization of the UBC gene from lotus (Nelumbo nucifera)
Authors: Y. Diao, G.L. Li, A.Q. Yu, X.W. Zheng, K.Q. Xie, Y.W. Wang, M.Q. Zhou, J. Ming and Z.L. Hu
Protein ubiquitination is extensively involved in the regulation of a considerable number of physiological processes in plant cells. E2 (ubiquitin-conjugating enzyme, UBC), one of the essential enzymes of eukaryotic ubiquitination, catalyzes protein ubiquitination together with E1 and E3. In this study, we cloned four full-length cDNANnUBCs of Nelumbo nuc.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038341
- DOI:
- 10.4238/gmr.15038341
Development of microsatellite markers and genetic diversity analysis for Pelodiscus sinensis
Authors: T. Li, J. Zhao, W. Li, Y. Shi, X.Y. Hong and X.P. Zhu,
Pelodiscus sinensis is a common freshwater soft-shell turtle found in China, and is an important aquaculture species. In this study, 20 polymorphic microsatellite primers were developed from the transcriptome. The genetic diversity of three populations of P. sinensis was evaluated, using 72 individuals. The number of alleles per locus ranged from 3 to 26... Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038346
- DOI:
- 10.4238/gmr.15038346
Characterization of microRNAs and their target genes associated with transcriptomic changes in gamma-irradiated Arabidopsis
Authors: J.H. Kim, Y.S. Go, J.K. Kim and B.Y. Chung
MicroRNAs (miRNAs) regulate gene expression in response to biotic and abiotic stress in plants. We investigated gamma-ray-responsive miRNAs in Arabidopsis wild-type and cmt3-11t mutant plants using miRNA microarray analysis. miRNA expression was differentiated between the wild-type and cmt3-11t mutants. miR164a, miR169d, miR169h, miR172b*, and miR403 were.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038386
- DOI:
- 10.4238/gmr.15038386
Genetic diversity of Toona sinensis Roem in China revealed by ISSR and SRAP markers
Authors: P.Y. Xing, T. Liu, Z.Q. Song and X.F. Li
Toona sinensis Roem has an important value as a type of traditional vegetable and Chinese medicinal herb, and is also a valuable source of wood in China. In this study, we used the inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers to assess the level and pattern of genetic diversity in five domesticated T. sine.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038387
- DOI:
- 10.4238/gmr.15038387