Expression
Purification, characterization, and heterologous expression of an antifungal protein from the endophytic Bacillus subtilis strain Em7 and its activity against Sclerotinia sclerotiorum
Authors: N.N. Wang, X.N. Gao, X. Yan, Z.P. Li, Z.S. Kang, L.L. Huang and Q.M. Han
An antifungal protein exhibiting a high activity against Sclerotinia sclerotiorum in vivo was purified by ammonium sulfate precipitation, hydrophobic chromatography, and gel filtration chromatography from the culture filtrate of the endophytic Bacillus subtilis strain Em7. The protein was characterized as a β-1,3-1,4.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.30.27
- DOI:
- 10.4238/2015.November.30.27
Analysis of gene expression patterns and levels in maize hybrids and their parents
Authors: H.S. Nie, S.P. Li, X.H. Shan, Y. Wu, S.Z. Su, H.K. Liu, J.Y. Han and Y.P. Yuan
Heterosis has greatly contributed to conventional plant breeding and is widely used to increase crop plant productivity. However, although some studies have explored the mechanisms of heterosis at the genomic and transcriptome level, these mechanisms still remain unclear. The growth and development of maize seedlings and immat.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.30.17
- DOI:
- 10.4238/2015.November.30.17
Molecular characterization and expression patterns of insulin-like growth factor-binding protein genes in postnatal Nanjiang brown goats
Authors: S.Y. Zhan, L. Chen, L. Li, L.J. Wang, T. Zhong and H.P. Zhang
Insulin-like growth factor-binding proteins (IGFBPs) play a key role in modulating insulin-like growth factors (IGFs), and are considered candidate genes for growth traits in livestock. In this study, we identified the complete coding sequences of IGFBP-1 to -6 in the Nanjiang brown goat, and assessed gene tissue expression pa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.16.22
- DOI:
- 10.4238/2015.October.16.22
Intestinal lactic acid bacteria from Muscovy duck as potential probiotics that alter adhesion factor gene expression
Authors: Z.L. Xie, D.P. Bai, L.N. Xie, W.N. Zhang, X.H. Huang and Y.F. Huang
The purpose of this study was to assess the suitability of lactic acid bacteria (LABs) isolated from Muscovy duck as a potential probiotic. Isolates were identified by targeted polymerase chain reaction and assessed in vitro for probiotic characteristics such as autoaggregation; surface-charge; hydrophobicity; tolerance to aci.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.15
- DOI:
- 10.4238/2015.October.9.15
Cloning and prokaryotic expression of the porcine lipasin gene..
Authors: M.M. Li* J. Geng, Y.J. Guo, X.Q. Jiao, W.F. Lu, H.S. Zhu, Y.Y. Wang and G.Y. Yang
Lipasin has recently been demonstrated to be involved in lipid metabolism. In this study, two specific primers were used to amplify the lipasin open reading frame from porcine liver tissue. The polymerase chain reaction product was cloned to a pGEM®-T Easy Vector, digested by SalI and NotI, and sequenced. The lipasin fragment was then cloned to a pET2.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.34
- DOI:
- 10.4238/2015.November.18.34
Immunological characteristics of outer membrane protein omp31 of goat Brucella and its monoclonal antibody
Authors: W.Y. Zheng, Y. Wang, Z.C. Zhang and F. Yan
We examined the immunological characteristics of outer membrane protein omp31 of goat Brucella and its monoclonal antibody. Genomic DNA from the M5 strain of goat Brucella was amplified by polymerase chain reaction and cloned into the prokaryotic expression vector pGEX-4T-1. The expression and immunological characteristics of .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.10
- DOI:
- 10.4238/2015.October.5.10
Gene expression profiles of the heterotrophic microalga Chlorella pyrenoidosa F-9
Authors: X. Sun1, N.J. Xu1, L.Z. Jiang1, W. Hu1, M.H. Fan1 and Z.M. Liu2
A strain of the microalga Chlorella pyrenoidosa F-9 in our laboratory showed special characteristics when transferred from autotrophic to heterotrophic culture. In order to elucidate the possible metabolic mechanism, the gene expression profiles of the autonomous organelles in the green alga C. pyrenoidosa under autotrophic and heterotrophic cultivation w.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.20.17
- DOI:
- 10.4238/2014.October.20.17
Molecular cloning and expression analysis of five GhRAXs in upland cotton (Gossypium hirsutum L.)
Authors: T.C. Dai and Z.M. Wang
The formation of axillary meristems in leaf axils is a prerequisite for the development of lateral shoots, which largely contribute to plant architecture. Several transcription factor-encoding genes, including CUC3, RAX, LAS, LOF1, and ROX, have been cloned by screening for axillary meristem mutants in Arabidopsis thaliana. Th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.25
- DOI:
- 10.4238/2015.October.5.25
Expression patterns of Doppel in differential ovine PRNP genotypes: quantification using real-time RT-PCR
Authors: C. Wang, C.-L. Zhao, L. Liu, R. Wu and X.-L. Zhang
Doppel is a homologue of cellular prion protein (PrP)-like protein (PrPC). Different tissue samples were collected from the central nervous system plus four regions of lymphoid system, eleven regions of digestive tract and two reproductive organs from four ARR/ARQ and four ARH/ARQ sheep, genotypes of the PrP gene. Total RNA wa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.3
- DOI:
- 10.4238/2015.October.9.3
ZNF797 plays an oncogenic role in gastric cancer
Authors: D. Momenzadeh1, S. Rahman Zadeh1, M. Rezaei-Tavirani1, A. Baradaran-Rafii2, F. Ghasemvand1 and S. Heidari-Keshel1
Human cancer cells resemble stem cells in expression signatures leading them to share some features, most notably, selfrenewal. A complex network of transcription factors and signaling molecules are required for continuation of this trait. ZNF797 (SALL4) is a zinc finger transcriptional activator crucial for maintenance of self-renewal in stem cells; howe.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.20.18
- DOI:
- 10.4238/2014.October.20.18