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Plant Genetics   Research Article

Isolation of fertility-related genes of multiple-allele-inherited male sterility in Brassica rapa ssp pekinensis by cDNA-AFLP

Authors: R.Q. Ji, Q. Song, X.F. Xin, X. Zhou and H. Feng

To better understand the molecular mechanisms of multiple-allele-inherited male sterility in Chinese cabbage (Brassica rapa ssp pekinensis), differentially expressed genes in fertile and sterile plants must be isolated. We used cDNA-AFLP analysis to isolate differentially expressed genes in fertile and sterile buds of the two-.. Read More»

Genet. Mol. Res. 10(4):
2011.December.5.8
DOI:
10.4238/2011.December.5.8
Plant Genetics   Research Article

Molecular identification of Fusarium species isolated from transgenic insect-resistant cotton plants in Mexicali valley, Baja California

Authors: T. Gonzalez-Soto, D. Gonz�¡lez-Mendoza, R. Troncoso-Rojas, A. Morales-Trejo, C. Cece�±a-Duran, A. Garcia-Lopez and O. Grimaldo-Juarez

Cotton production in the Mexicali valley is adversely affected by wilt and root rot disease associated with Fusarium species. In the present study, we sought to isolate and identify the Fusarium species in the rhizosphere of transgenic insect-resistant cotton plants grown in the Mexicali valley. Our analyses isolated four nati.. Read More»

Genet. Mol. Res. 14(4):
2015.October.2.7
DOI:
10.4238/2015.October.2.7
Plant Genetics   Research Article

Genetic characterization of heat tolerant tomato (Solanum lycopersicon) genotypes by SRAP and RAPD markers

Authors: N. Comlekcioglu, O. Simsek, M. Boncuk and Y. Aka-Kacar

We employed RAPD and sequence-related amplified polymorphism (SRAP) markers to evaluate polymorphisms in 15 tomato (Solanum lycopersicon) genotypes that were obtained from a tomato breeding program. Four local tomato genotypes selected from the Sanliurfa province (Southeastern Anatolia Region of Turkey), 10 heat-tolerant tomat.. Read More»

Genet. Mol. Res. 9(4):
vol9-4gmr876
DOI:
10.4238/vol9-4gmr876
Plant Genetics   Research Article

Transposable elements in Phyllostachys pubescens (Poaceae) genome survey sequences and the full-length cDNA sequences, and their association with simple-sequence repeats

Authors: M.B. Zhou, X.M. Liu, and D.Q. Tang

Phyllostachys pubescens is a woody bamboo with the highest ecological, economic, and cultural values of all bamboos in Asia. There is more genomic data available for P. pubescens than for any other bamboo species, including 2.12-Mb genome survey sequences (GSS) and 11.4-Mb full-length cDNA sequences (FL-cDNAs) currently deposi.. Read More»

Genet. Mol. Res. 10(4):
2011.December.6.3
DOI:
10.4238/2011.December.6.3
Animal Genetics   Research Article

Methylation status and chromatin structure of the myostatin gene promoter region in the sea perch Lateolabrax japonicus (Perciformes)

Authors: E.M. Abbas, A. Takayanagi, N. Shimizu and M. Kato

Myostatin is a negative regulator of the growth and development of skeletal muscle mass. In fish, myostatin is expressed in several organs in addition to skeletal muscle. To understand the mechanisms regulating myostatin gene expression in the sea perch, Lateolabrax japonicus, we examined the methylation status of the myostatin gene promoter region in sev.. Read More»

Genet. Mol. Res. 10(4):
2011.December.8.7
DOI:
10.4238/2011.December.8.7
Human Genetics   Research Article

Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene

Authors: E.G. Lima, R.C. Lira, A.L.S. Jesus, R. Dhalia and A.C. Freitas

Papillomaviruses are known to cause tumor lesions, generally benign, in epithelial tissues of diverse organisms; these lesions may progress to cancer under suitable conditions. Bovine papillomavirus (BPV) can cause urinary bladder cancer and cancer of the upper gastrointestinal tract. Furthermore, BPV1 and BPV2 are implicated .. Read More»

Genet. Mol. Res. 13(1):
http://dx.doi.org/2014.February.20.13
DOI:
http://dx.doi.org/10.4238/2014.February.20.13
Microbial Genetics   Research Article

Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)

Authors: S.A. Rhoden, A. Garcia, M.C. Santos e Silva, J.L. Azevedo and J.A. Pamphile

Various organisms such as fungi and bacteria can live inside plants, inhabiting the aerial parts (primarily the leaves) without causing damage. These microorganisms, called endophytes, produce an extensive variety of compounds that can be useful for medical and agronomic purposes. Trichilia elegans A. Juss., belonging to the Meliaceae family, shows wide d.. Read More»

Genet. Mol. Res. 14(1):
2015.February.20.7
DOI:
10.4238/2015.February.20.7
Microbial Genetics   Research Article

An efficient method for DNA extraction from Cladosporioid fungi

Authors: M.A. Moslem, A.H. Bahkali, K.A. Abd-Elsalam and P.J.G.M. Wit

We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells. In order to overcome this we grew these fungi for three days on agar plates and extracted D.. Read More»

Genet. Mol. Res. 9(4):
vol9-4gmr936
DOI:
10.4238/vol9-4gmr936
Plant Genetics   Research Article

Selection of DNA barcoding loci for Nepeta deflersiana Schweinf. ex Hedge from chloroplast and nuclear DNA genomes

Authors: F. Al-Qurainy, S. Khan, M. Nadeem, M. Tarroum and A.R.Z. Gaafar

Molecular markers, mainly DNA-based are potential tools for DNA barcoding and phylogenetic study. The plant species belonging to the Nepeta genus have important medicinal value because of the presence of nepetalactones, and they have been used to treat human diseases. We amplified nuclear and chloroplast gene loci to develop a.. Read More»

Genet. Mol. Res. 13(1):
http://dx.doi.org/2014.February.21.3
DOI:
http://dx.doi.org/10.4238/2014.February.21.3
Animal Genetics   Research Article

DNA genetic artificial fish swarm constant modulus blind equalization algorithm and its application in medical image processing

Authors: Y.C. Guo, H. Wang and B.L. Zhang

This study proposes use of the DNA genetic artificial fish swarm constant modulus blind equalization algorithm (DNA-G-AFS-CMBEA) to overcome the local convergence of the CMBEA. In this proposed algorithm, after the fusion of the fast convergence of the AFS algorithm and the global search capability of the DNA-G algorithm to dr.. Read More»

Genet. Mol. Res. 14(4):
2015.October.2.14
DOI:
10.4238/2015.October.2.14