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Plant Genetics   Research Article

Genetic effects of in vitro germination and plantlet development in chilli pepper

Authors: P.A. Barroso, M.M. Rêgo, E.R. Rêgo and K.T.C. Ferreira

The objective of this study was to estimate the genetic effects on in vitro germination and development of chili peppers. For this, four genotypes (UFPB-132, -134, -137, and -390) were used as parents. They were crossed in a complete diallel scheme. The parents (4) and hybrid (12) seeds were germinated in glass bottles contain.. Read More»

Genet. Mol. Res. 16(3):
Plant Genetics   Research Article

Molecular characteristics and cloning of two pepper genes AN2 and UPA20

Authors: H.X. Chen, H. Jiang, J.L. Huo, J.F. Wen, H.S. Zhu, C.H. Ma, H. Zhou, J.H. Lv and M.H. Deng

The complete coding sequences (CDSs) of “Yunnan Purple Pepper No.1” (Capsicum annuum L.) AN2 and UPA20 genes were amplified using the reverse transcriptase polymerase chain reaction on the basis of the conserved sequence information of some Solanaceae plants and known highly homologous pepper expressed sequence tags. The nucleotide sequence an.. Read More»

Genet. Mol. Res. 13(2):
Plant Genetics   Research Article

Characterizing and marker-assisting a novel chili pepper (Capsicum annuum L.) yellow bud mutant with cytoplasmic male sterility

Authors: G.S. Sun, Z.L. Dai, P.W. Bosland, Q. Wang, C.Q. Sun, Z.C. Zhang and Z.H. Ma

Cytoplasmic male sterility (CMS) in pepper is a better way to produce hybrid seeds compared to manual production. We used the two sequence characterized amplified region (SCAR) markers (CRF-SCAR and CMS-SCAR130) in CMS pepper, to identify the genotype. We assembled two CMS yellow bud mutants (YBM; YBM12-A and YBM12-B). This mu.. Read More»

Genet. Mol. Res. 16(1):
Human Genetics   Research Article

Molecular cloning, sequence characterization of a novel pepper gene NADP-ICDH and its effect on cytoplasmic male sterility

Authors: M.H. Deng J.F. Wen J.L. Huo H.S. Zhu X.Z. Dai Z.Q. Zhang H. Zhou X.X. Zou

NADP-dependent isocitrate dehydrogenase (NADP-ICDH) is an important enzyme involved in energy metabolism. The complete coding sequence of the pepper (Capsicum annuum) NADP-ICDH gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. Nuc.. Read More»

Genet. Mol. Res. 11(3):
Plant Genetics   Research Article

Cloning and expression analysis of pepper chlorophyll catabolite reductase gene CaRCCR

Authors: H.-J. Xiao, J.-H. Jin, W.-G. Chai and Z.-H. Gong

Opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase (RCCR). In this study, a novel RCCR gene, CaRCCR, was isolated from the pepper (Capsicum annuum L... Read More»

Genet. Mol. Res. 14(1):
Plant Genetics   Research Article

Novel and highly informative Capsicum SSR markers and their cross-species transferability

Authors: G.S.C. Buso, A.M.M. Reis, Z.P.S. Amaral and M.E. Ferreira

This study was undertaken primarily to develop new simple sequence repeat (SSR) markers for Capsicum. As part of this project aimed at broadening the use of molecular tools in Capsicum breeding, two genomic libraries enriched for AG/TC repeat sequences were constructed for Capsicum annuum. A total of 475 DNA clones were sequenced from both libraries and 1.. Read More»

Genet. Mol. Res. 15(3):
Plant Genetics   Research Article

Cloning and bioinformatic analysis of full-length novel pepper (Capsicum annuum) genes TAF10 and TAF13

Authors: J.F. Wen, J.L. Huo, H.X. Chen, C.H. Ma, H. Jiang, H.S. Zhu, H. Zhou and M.H. Deng

We isolated two TATA-binding protein-associated factor (TAF) genes, TAF10 and TAF13, from pepper (Capsicum annuum). The complete coding sequences were amplified using reverse transcriptase-PCR on the basis of conserved sequence information of eggplant and several other plant species. Nucleotide sequence analysis of these two genes revealed that the pepper.. Read More»

Genet. Mol. Res. 12(4):
Plant Genetics   Research Article

Cloning and expression analysis of heat-shock transcription factor gene CaHsfA2 from pepper (Capsicum annuum L.)

Authors: M. Guo, Y.-X. Yin, J.-J. Ji, B.-P. Ma, M.-H. Lu, and Z.-H. Gong,

The heat-shock transcription factor (Hsf) gene CaHsfA2 (GenBank accession No. JX402923) was cloned from the Capsicum annuum thermotolerant line R9 by combining the techniques electron cloning and rapid amplification of cDNA ends. The gene, which is 1436 bp in length, had an open reading frame of 1089 bp that encoded 362 amino .. Read More»

Genet. Mol. Res. 13(1):
Cytogenomics   Research Article

Genetic determinants of the defense response of resistant and susceptible pepper (Capsicum annuum) cultivars infected with Phytophthora capsici (Oomycetes; Pythiaceae)

Authors: Y.-L. Zhang, D.-W. Li, Z.-H. Gong, J.-E. Wang, Y.-X. Yin and J.-J. Ji

Based on culture isolation and morphological observation blight-infected pepper plants in Shaanxi Province, China, we identified the pathogen causing pepper phytophthora blight as Phytophthora capsici. Varieties that differed in resistance (CM334, PBC602, and B27) were inoculated with this pathogen. The root activity of resistant CM334 variety was the hig.. Read More»

Genet. Mol. Res. 12(3):
Medical Genetics   Research Article

A molecular marker for in situ genetic resource conservation of Capsicum annuum var. acuminatum (Solanaceae)

Authors: N. Kaewdoungdee and T. Tanee

The Thailand cultivar pepper ‘phrik man bangchang’ (Capsicum annuum var. acuminatum, Solanaceae) was originally cultivated in the Bangchang Subdistrict, Amphawa District in Samut Songkhram Province. The cultivated areas are limited; we verified its distribution in Thailand for in situ ‘phrik man bangchang’ genetic resource conserva.. Read More»

Genet. Mol. Res. 12(3):