Biotechnology
Genetic diversity and pectinolytic activity of epiphytic yeasts from grape carposphere
Authors: M. Ciliao Filho, M.B.D. Berteli, J.S. Valle, L.D. Paccola-Meirelles, G.A. Linde, F.G. Barcellos and N.B. Colauto
The genetic diversity of epiphytic yeasts from grape carposphere is susceptible to environmental variations that determine the predominant carposphere microbiota. Understanding the diversity of yeasts that inhabit grape carposphere in different environments and their pectinolytic activity is a way to understand the biotechnolo.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029698
- DOI:
- 10.4238/gmr16029698
Molecular characterization by amplified ribosomal DNA restriction analysis and antimicrobial potential of endophytic fungi isolated from Luehea divaricata (Malvaceae) against plant pathogenic fungi and pathogenic bacteria
Authors: J. Bernardi-Wenzel1, A. Garcia2, J.L. Azevedo2 and J.A. Pamphile2
Luehea divaricata is an important plant in popular medicine; it is used for its depurative, anti-inflammatory, and other therapeutic activities. We evaluated the antimicrobial activity of endophytic fungi isolated from leaves of L. divaricata against phytopathogens and pathogenic bacteria, and characterized the isolates based on amplified ribosomal DNA re.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.29.2
- DOI:
- 10.4238/2013.October.29.2
Protein families, natural history and biotechnological aspects of spider silk
Authors: D. Bittencourt, P.F. Oliveira, F. Prosdocimi and E.L. Rech
Spiders are exceptionally diverse and abundant organisms in terrestrial ecosystems and their evolutionary success is certainly related to their capacity to produce different types of silks during their life cycle, making a specialized use on each of them. Presenting particularly tandemly arranged amino acid repeats, silk proteins (spidroins) have mechanic.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.10
- DOI:
- 10.4238/2012.August.13.10
Genetically modified crops: Brazilian law and overview
Authors: C.D. Marinho, F.J.O. Martins, A.T. Amaral J�ºnior, L.S.A. Gon�§alves, O.J.A.P. dos Santos, D.P. Alves, B.P. Brasileiro and L.A. Peternelli
In Brazil, the first genetically modified (GM) crop was released in 1998, and it is estimated that 84, 78, and 50% of crop areas containing soybean, corn, and cotton, respectively, were transgenic in 2012. This intense and rapid adoption rate confirms that the choice to use technology has been the main factor in developing nat.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.7.15
- DOI:
- 10.4238/2014.July.7.15
Characterization of chloroplast region rrn16-rrn23S from the tropical timber tree Cedrela odorata L. and de novo construction of a transplastomic expression vector suitable for Meliaceae trees and other economically important crops
Authors: L.A. LÃ?³pez-Ochoa, M.M. Apolinar-HernÃ?¡ndez and Y.J. PeÃ?±a-RamÃ?Ârez
The forest tree Spanish cedar (Cedrela odorata L.) is well-known for its high-value timber; however, this species is attacked by the shoot borer (Hypsipyla grandella) during its early years of development, resulting in branched stems and making the plants useless for high-quality wood production. The generation of resistant varieties expressing entomotoxi.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.20.2
- DOI:
- 10.4238/2015.February.20.2
Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)
Authors: S.A. Rhoden, A. Garcia, M.C. Santos e Silva, J.L. Azevedo and J.A. Pamphile
Various organisms such as fungi and bacteria can live inside plants, inhabiting the aerial parts (primarily the leaves) without causing damage. These microorganisms, called endophytes, produce an extensive variety of compounds that can be useful for medical and agronomic purposes. Trichilia elegans A. Juss., belonging to the Meliaceae family, shows wide d.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.20.7
- DOI:
- 10.4238/2015.February.20.7
Genetically modified animals for use in research and biotechnology
Authors: L.M. Chaible, M.A. Corat, E. Abdelhay and M.L.Z. Dagli
Transgenic animals are used extensively in the study of in vivo gene function, as models for human diseases and in the production of biopharmaceuticals. The technology behind obtaining these animals involves molecular biology techniques, cell culture and embryo manipulation; the mouse is the species most widely used as an expe.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr867
- DOI:
- 10.4238/vol9-3gmr867
Biotechnological prospecting of foliar endophytic fungi of guaco (Mikania glomerata Spreng.) with antibacterial and antagonistic activity against phytopathogens
Authors: J.C. Polonio, T.T. Almeida, A. Garcia, G.E.G. Mariucci, J.L. Azevedo, S.A. Rhoden and J.A. Pamphile
Mikania glomerata (Spreng.), popularly known as “guaco”, is a plant from the Asteraceae family that has many therapeutic properties. The use of medicinal plants has been examined in studies on endophytic diversity and bioprospecting; endophytes inhabit the interior of plants without harming them. Microorganism-host complex interactions are rel.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.5
- DOI:
- 10.4238/2015.July.3.5
In silico analysis of the 16S rRNA gene of endophytic bacteria, isolated from the aerial parts and seeds of important agricultural crops
Authors: C. Bredow, J.L. Azevedo, J.A. Pamphile, C.A. Mangolin and S.A. Rhoden
Because of human population growth, increased food production and alternatives to conventional methods of biocontrol and development of plants such as the use of endophytic bacteria and fungi are required. One of the methods used to study microorganism diversity is sequencing of the 16S rRNA gene, which has several advantages, including universality, size.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.3
- DOI:
- 10.4238/2015.August.19.3
Update of microbial genome programs for bacteria and archaea
Authors: Patricia Borges Santos Celestino, Lydston Rodrigues de Carvalho,Leandro Martins de Freitas, Fernanda Alves Dorella, Natalia Flor�ªncio Martins, Luiz Gustavo Carvalho Pacheco, Anderson Miyoshi and Vasco Azevedo
Since the Haemophilus influenzae genome sequence was completed in 1995, 172 other prokaryotic genomes have been completely sequenced, while 508 projects are underway. Besides pathogens, organisms important in several other fields, such as biotechnology and bioremediation, have also been sequenced. Institutions choose the organ.. Read More»
- Genet. Mol. Res. 3(3):