Microsatellite markers for assessing genetic diversity of the medicinal plant Paris polyphylla var. chinensis (Trilliaceae)
Authors: J.Y. Zheng, H. Wang, X.X. Chen, P. Wang, P. Gao, X.N. Li and G.P. Zhu
Paris polyphylla var. chinensis is a perennial herb with medicinal properties that is widely used in traditional Chinese medicine. However, this plant has been on the edge of extinction during the last few decades because of excessive deforestation based on the intense ethnopharmaceutical interest. We isolated 12 microsatellit.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.6.1
- DOI:
- 10.4238/2012.August.6.1
Relationship of common expression quantitative trait loci genes to the immune system
Authors: Relationship of common expression quantitative trait loci genes to the immune system
Tissue-specific expression quantitative trait loci (eQTLs) are always linked to specific diseases. In this study, we focused on eQTLs common to multiple tissues and explored their functional mechanisms in disease for the first time. We found 11 common eQTL genes among multiple tissues. Five genes were validated through a genome-wide association study, 3 g.. Read More»
- Genet. Mol. Res. 12(4):
Detection of bacterial blight resistance genes in basmati rice landraces
Authors: I. Ullah, S. Jamil, M.Z. Iqbal, H.L. Shaheen, S.M. Hasni, S. Jabeen, A. Mehmood and M. Akhter
Aromatic basmati rice is vulnerable to bacterial blight disease. Genes conferring resistance to bacterial blight have been identified in coarse rice; however, their incorporation into basmati varieties compromises the prized basmati aroma. We identified bacterial blight resistance genes Xa4, xa5, Xa7, and xa13 in 52 basmati la.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.20.1
- DOI:
- 10.4238/2012.July.20.1
A family-based association study identified CYP17 as a candidate gene for obesity susceptibility in Caucasians
Authors: H. Yan, Y. Guo, T.-L. Yang, L.-J. Zhao and H.-W. Deng
The cytochrome P450c17α gene (CYP17) encodes a key biosynthesis enzyme of estrogen, which is critical in regulating adipogenesis and adipocyte development in humans. We therefore hypothesized that CYP17 is a candidate gene for predicting obesity. In order to test this hypothesis, we performed a family-based association t.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.22.1
- DOI:
- 10.4238/2012.May.22.1
Application and effects of mouse Foxp3 antibody and fixation/permeabilization buffer on the detection of CD4+ regulatory T cells in various mammal species
Authors: Y.L. Sun, G.G. Lin, K. Zhang, L.N. Wang and J.M. Li
CD4+ regulatory T lymphocytes (Treg cells) play a crucial role in maintaining the normal immune homeostasis. Foxp3, as a key marker for Treg cells, is widely used to identify Treg cells, not only in humans but also in other species, like mouse, porcine, ovine, and bovine. To detect reproducible Treg cells is important for evaluating the state of the immun.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.11.5
- DOI:
- 10.4238/2013.December.11.5
Development and characterization of microsatellite markers for the Chinese endangered medicinal plant Tetrastigma hemsleyanum
Authors: Y.H. Wang, N. Chen, Y.C. Zhang and C.X. Fu
Tetrastigma hemsleyanum (Vitaceae) is an endangered medicinal plant endemic to China. Because of its widely known efficacy for treating many health problems, wild resources of this species are currently undergoing a rapid decline. Few studies have been conducted examining the population genetics or development of microsatellite loci for this plant. In thi.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.31.21
- DOI:
- 10.4238/2014.October.31.21
Breeding biofortified cowpea lines for semi-arid tropical areas by combining higher seed protein and mineral levels
Authors: C.A.F. Santos and L.S. Boiteux
One strategy to mitigate human malnutrition in semi-arid areas is to increase the protein and mineral content of cowpea cultivars. Total seed protein, potassium, calcium, iron, zinc, and sodium contents were quantified in elite cowpea lines, with the aim to develop cultivars that had improved levels of these nutrients. Eighty-seven F6 lines derived from 6.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.16.4
- DOI:
- 10.4238/2013.December.16.4
Isolation of novel microsatellite markers from Paralichthys lethostigma (Paralichthyidae) and their cross-species application in Pleuronectiformes
Authors: Y.-J. Xu, M.-J. Liao, Y.-G. Wang, Z.-C. Liu, P. Qin, Z. Zhang, X.-J. Rong and X.-Z. Liu
We investigated the genetic diversity of the southern flounder Paralichthys lethostigma. Microsatellite-enriched libraries were constructed and novel microsatellite markers were developed and applied for genetic detection of wild populations. Cross-species amplification was also conducted in five pleuronectiforme species. Of 45 randomly selected and seque.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.16.2
- DOI:
- 10.4238/2013.December.16.2
Frequencies of -308G/A (TNFA) and -509C/T (TGFB1) polymorphisms in sickle cell anemia patients from Brazil
Authors: L.S. Torres, E. Belini Junior, D.G. Silva, C.L. Lobo, M.A. Ruiz and C.R. Bonini-Domingos
Sickle cell anemia is an affection that causes chronic inflammation, with consequences for vaso-occlusion, oxidative stress and cytokine production. Genetic polymorphisms in markers involved in this process can modulate the inflammatory response, including polymorphisms -308G/A of TNFA (tumor necrosis factor alpha) and -509C/T of TGFB1 (transforming growt.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.16.1
- DOI:
- 10.4238/2013.December.16.1
Inheritance of downy mildew (Plasmopara viticola) and anthracnose (Sphaceloma ampelinum) resistance in grapevines
Authors: O. Poolsawat, S. Mahanil, P. Laosuwan, S. Wongkaew,A. Tharapreuksapong, B.I. Reisch and P.A. Tantasawat
Downy mildew (Plasmopara viticola) and anthracnose (Sphaceloma ampelinum) are two of the major diseases of most grapevine (Vitis vinifera L.) cultivars grown in Thailand. Therefore, breeding grapevines for improved downy mildew and anthracnose resistance is crucial. Factorial crosses were made between three downy mildew and/or anthracnose resistant lines .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.13.8
- DOI:
- 10.4238/2013.December.13.8