Study on the treatment of the p15 gene combined with Bcr-abl-specific siRNA and STI571 for chronic myeloid leukemia
Authors: W. Wang, Y. Du, N.-N. Li, F.-F. Lv and G.-Q. Lin
The aim of this study was to investigate the effect of the p15 gene combined with Bcr-abl-specific siRNA and STI571 on the proliferation, cell cycle and apoptosis of K562 chronic myeloid leukemia cells. Using the gene sequence results, we amplified the p15 gene from normal peripheral blood by RT-PCR, and constructed a p15-pcDN.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.4
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.4
Interleukin-10 gene -592C>A polymorphism and susceptibility to gastric cancer
Authors: M. Qi, D.M. Liu, L.L. Pan and Y.X. Lin
Numerous studies have evaluated the association between the human interleukin-10 gene -592C>A polymorphism and gastric cancer risk. However, the results have been inconsistent. This meta-analysis was designed to resolve these controversies. Systematic searches of the electronic databases Embase, PubMed, and Google Scholar were performed to identify rel.. Read More»
- Genet. Mol. Res. 13(4):
Effects of genetic variants of CCR5 chemokine receptors on oral squamous cell carcinoma
Authors: C.R. Tanyel1, Z.B. Cincin2, B. Gokcen-Rohlig1, K. Bektas-Kayhan3, M. Unur3 and B. Cakmakoglu2
We aimed to evaluate the effect of genetic variants of the chemokine C-C motif receptor (CCR5) in the pathogenesis of oral squamous cell carcinoma (OSCC). A total of 127 patients diagnosed with OSCC and 104 healthy individuals were included in the study. The polymorphisms CCR5 59029 and CCR5-delta32 were assessed with the polymerase chain reaction-restric.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.20
- DOI:
- 10.4238/2013.November.18.20
Validation of quantitative fluorescent-PCR for rapid prenatal diagnosis of common aneuploidies in the Chinese population
Authors: A.-Q. Xu, M. Xia, J.-T. Liu, F.-X. Yao, W.-M. Zhang, N. Hao, J. Zhou and X.-M. Bian
Quantitative fluorescent polymerase chain reaction (QF-PCR) is an accurate and reliable method for rapid detection of aneuploidy; however, it is not routinely used in China. We aimed to validate QF-PCR as a means for prenatal common aneuploidy screening and to analyze the heterozygosities of short tandem repeat (STR) markers in the Chinese population. The.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.9.1
- DOI:
- 10.4238/2013.December.9.1
Comparison of computed tomography and magnetic resonance imaging in the evaluation of facet tropism and facet arthrosis in degenerative cervical spondylolisthesis
Authors: C. Xu, Z.H. Ding and Y.K. Xu
The aim of this study was to determine the reliability of magnetic resonance imaging (MRI) in the assessment of facet tropism and facet arthrosis of spondylolisthesis levels in degenerative cervical spondylolisthesis as compared to computed tomography (CT). The discrepancies in the interpretation of CT and MRI data in the eval.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.5
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.5
Isolation and identification of bovine primary hepatocytes
Authors: Q.D. Jiang1, H.P. Li2, F.J. Liu2, X.J. Wang2, Y.J. Guo2, L.F. Wang2, W.F. Lu2, H.J. Li2, X.P. Li1 and G.Y. Yang2
The liver is a unique organ that is endowed with a plethora of specialized functions. Most of its functional traits are controlled by hepatocytes. Primary hepatocytes have been used widely in in vitro models to understand the biological processes occurring in the liver. There are a number of methods used to separate hepatocytes, but the cell activity and .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.30.3
- DOI:
- 10.4238/2013.October.30.3
Association between CASP-8 gene polymorphisms and cancer risk in some Asian population based on a HuGE review and meta-analysis
Authors: Y.J. Zhang, X.P. Zhong, Y. Chen, S.R. Liu, G. Wu and Y.F. Liu
Genetic variation in the CASP-8 gene reportedly can increase cancer susceptibility by regulating tumor cell proliferation and apoptosis. Several studies have investigated this possibility; however, the conclusions remain controversial. We made a Human Genome Epidemiology (HuGE) review and did a meta-analysis to explore the association between CASP-8 gene .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.3
- DOI:
- 10.4238/2013.February.28.3
Expression and purification of GST-FHL2 fusion protein
Authors: H. Yu, Q. Ma, J. Lin, Y.F. Sun and F. Zheng
Escherichia coli is the most widely used host for the production of recombinant proteins. However, most eukaryotic proteins are typically obtained as insoluble, misfolded inclusion bodies that need solubilization and refolding. The interactions between human FHL2 protein and many types of proteins, including structural proteins, kinases, and several class.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.6.4
- DOI:
- 10.4238/2013.December.6.4
Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
Authors: F.J. Bello, N.A. Segura and M. Ruiz-Garcia
Ochlerotatus taeniorhynchus (Diptera: Culicidae) is a mosquito, which is an efficient vector of the virus causing epidemic-epizootic Venezuelan equine encephalitis in Colombia. This study used 9 random amplified polymorphic DNA (RAPD) markers to analyze the mosquito’s genetic variability and genetic structure of 122 spec.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.6
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.6
Genetic diversity and relationships among accessions of five crested wheatgrass species (Poaceae: Agropyron) based on gliadin analysis
Authors: S.Y. Chen, X. Ma, X.Q. Zhang, L.K. Huang and J.N. Zhou
Agropyron Gaertn. is the most important genus in Triticeae (Poaceae), which includes many forage grasses with high economic value. The genetic diversity and relationships of 36 accessions from five crested wheatgrass species were analyzed by gliadin markers. A total of 54 product bands were detected after acid polyacrylamide gel electrophoresis (A-PAGE), .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.19
- DOI:
- 10.4238/2013.November.18.19