Psorophora columbiae and Psorophora toltecum (Diptera: Culicidae) Colombian populations cannot be differentiated by isoenzymes
Authors: Manuel Ruiz-Garcia, Diana Ramirez, Felio Bello, Diana Alvarez
Two populations of the mosquito Psorophora columbiae from the central Andean area of Colombia and one population of Ps. toltecum from the Atlantic coast of Colombia were analyzed for 11 isoenzyme markers. Psorophora columbiae and Ps. toltecum are two of the main vectors of Venezuelan equine encephalitis. We found no conspicuou.. Read More»
- Genet. Mol. Res. 2(2):
The anticancer homeopathic composite â??Canova Methodâ? is not genotoxic for human lymphocytes in vitro
Authors: Igor C. Seligmann, Patr?cia D.L. Lima, Pl?nio C.S. Cardoso, Andr?© S. Khayat, Marcelo O. Bahia, Dorli de Freitas Buchi Isabel R. Cabral, Rommel R. Burbano
The Canova Methodâ (CM) is a homeopathic medicine indicated for the treatment of patients with cancer and for pathologies that involve a depressed immune system, such as AIDS. This product is composed of homeopathic dilutions of Aconitum napellus, Arsenicum album (arsenic trioxide), Bryonia alba, Lachesis muta venom and .. Read More»
- Genet. Mol. Res. 2(2):
Cassava, Manihot esculenta Crantz genetic resources: VI. Anatomy of a diversity center
Authors: N.M.A. Nassar
Central Brasil is one of the four centers of diversity of Manihot species. Of 26 wild Manihot species reported to occur in this region, 20 were collected from two limited areas, each less than 100 km in diameter. These two areas are Goiás Velho and Corumbá de Goiás. Considering the Harlan concept of geogra.. Read More»
- Genet. Mol. Res. 2(2):
Diallel analysis of production traits among domestic, exotic and mutant germplasms of Lycopersicon
Authors: Guillermo Pratta, Liliana A. Picardi, Roxana Zorzoli
The effects of wild germplasm on tomato fruit shelf life have not yet been completely evaluated. Three different genotypes of Lycopersicon esculentum (a cultivated variety, a homozygote for nor and a homozygote for rin), LA1385 of L. esculentum var. cerasiforme, LA722 of L. pimpinellifolium, and 10 diallel hybrids were assayed.. Read More»
- Genet. Mol. Res. 2(2):
Birth of normal kids after microinjection of pronuclear embryos in a transgenic goat (Capra hircus) production program in Brazil
Authors: V.J.F. Freitas, I.A. Serova, L.E. Andreeva, E.S. Lopes J?ºnior, D.I.A. Teixeira, M.F. Cordeiro, D. Rondina, N.R.O. Paula, I.J. Arruda, J.B. Lima Verde, G. Dvoriantchikov, O. Serov
This pilot project was designed to determine if normal kids could be produced after microinjection in pronuclear embryos and subsequent transfer to recipients in a transgenic goat program in Brazil. Twelve donors of the Saanen breed and 17 recipients of an undefined breed were used. The estrus of both donors and recipients was.. Read More»
- Genet. Mol. Res. 2(2):
Comparative study of the cuticular hydrocarbon composition of Melipona bicolor Lepeletier, 1836 (Hymenoptera, Meliponini) workers and queens
Authors: F?¡bio C. Abdalla, Graeme R. Jones, Erik D. Morgan, Carminda da Cruz-Landim
In social insects, cuticular hydrocarbons are involved in species, kin, caste and nestmate recognition. Gas chromatography and mass spectrometry were used to compare the cuticular hydrocarbon composition of workers, males and queens of Melipona bicolor. The cuticular hydrocarbon composition of this species was found to consist.. Read More»
- Genet. Mol. Res. 2(2):
Anti-P53 antibodies in Brazilian brain tumor patients
Authors: Renata Fragelli Fonseca, M?¡rcia Tie Kawamura, Jos?© Ant?´nio Oliveira, Anselmo Teixeira, Gilda Alves, Maria da Gl?³ria da Costa Carvalho
Gliomas of astrocytic origin are the most common primary brain tumors, accounting for over 40 to 50% of all central nervous system tumors. The TP53 tumor suppressor gene is the most frequently mutated gene found in human malignancies. A mutation of this gene can lead to an increased half-life of the resulting protein and loss .. Read More»
- Genet. Mol. Res. 2(2):
Evaluation of a method for high yield purification of largely intact mitochondrial DNA from human placentae
Authors: Sergio U. Dani, Alessandra Cristina Gomes-Ruiz, Maria Angela C. Dani
We developed, and quantitatively and qualitatively evaluated an easily reproducible method for high yield purification of mitochondrial DNA (mtDNA) from human placentae by mechanical tissue disruption, differential centrifugation of mitochondria, enzymatic digestion, phenol extraction and ethanol precipitation. Average mtDNA y.. Read More»
- Genet. Mol. Res. 2(2):
Mining microorganism EST databases in the quest for new proteins
Authors: Alessandra Concei?§?£o Faria-Campos, Gustavo Coutinho Cerqueira, Charles Anacleto, Jos?© Miguel Ortega, Cl?¡udia M?¡rcia Benedetto de Carvalho
Microorganisms with large genomes are commonly the subjects of single-round partial sequencing of cDNA, generating expressed sequence tags (ESTs). Usually there is a great distance between gene discovery by EST projects and submission of amino acid sequences to public databases. We analyzed the relationship between available E.. Read More»
- Genet. Mol. Res. 2(1):
Stage-specific gene expression during Trypanosoma cruzi metacyclogenesis
Authors: Andr?©a Rodrigues ?vila, Bruno Dallagiovanna, Sueli Fumie Yamada-Ogatta, Viviane Monteiro-G?³es, St?ªnio Perdig?£o Fragoso Marco Aur?©lio Krieger, Samuel Goldenberg
The process of Trypanosoma cruzi metacyclogenesis involves the transformation of noninfective epimastigotes into metacyclic trypomastigotes, which are the pathogenic form. The analysis of stage-specific genes during T. cruzi metacyclogenesis may provide insight into the mechanisms involved in the regulation of gene expression .. Read More»
- Genet. Mol. Res. 2(1):