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Medical Genetics   Research Article

Comparison of high-intensity focused ultrasound therapy under nasal endoscopy guidance versus first-line drug treatment in patients with persistent allergic rhinitis

Authors: G.F. Feng, Z.L. Han, F. Wang, B.C. Sun, Z.Y. Dai, S.Z. Yang and C.Y. Zhou

The aim of this study was to determine whether high-intensity focused ultrasound (HIFU) therapy under nasal endoscopy guidance could provide better efficacy and safety in patients with persistent allergic rhinitis (PAR) than the first-line drugs recommended by the World Health Organization. A total of 120 adult patients with PAR were randomly divided into.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.20
DOI:
10.4238/2015.August.19.20
Human Genetics   Research Article

Heat shock protein 70 polymorphisms in Chinese patients with Graves� disease

Authors: Y.P. Wang, Z. Tang, B.K. Peng,Q. Zhen, S.L. Zhou and X.F. Jin

Graves’ disease (GD) is a common autoimmune disease mainly affecting the thyroid. However, the correlation between the development of GD and HSP70 alleles has not been reported in the Chinese population. Therefore, the aim of this study was to investigate the association between HSP70 polymorphisms and GD in the Chinese population. A total of 153 pa.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.25
DOI:
10.4238/2015.December.23.25
Human Genetics   Research Article

Quantitative trait locus analysis of grape weight and soluble solid content

Authors: Y.H. Zhao, Y.S. Guo, H. Lin, Z.D. Liu, H.F. Ma, X.W. Guo, K. Li, X.X. Yang, Z.Z. Niu and G.G. Shi

A grapevine hybrid population was derived from a crossing of the early-maturing female parent cultivar ‘87-1’ and the late-maturing male parent cultivar ‘9-22’. A total of 149 plants were selected from the hybrid population as the mapping population, and after sequence-related amplified polymorphism and simple-sequence repeat marke.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.21
DOI:
10.4238/2015.August.19.21
Plant Genetics   Research Article

Segregation analysis of microsatellite (SSR) markers in sugarcane polyploids

Authors: X. Lu, H. Zhou, Y.-B. Pan, C.Y. Chen, J.R. Zhu, P.H. Chen, Y.-R. Li, Q. Cai and R.K. Chen

No information is available on segregation analysis of DNA markers involving both pollen and self-progeny. Therefore, we used capillary electrophoresis- and fluorescence-based DNA fingerprinting together with single pollen collection and polymerase chain reaction (PCR) to investigate simple sequence repeat (SSR) marker segregation among 964 single pollens.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.26
DOI:
10.4238/2015.December.23.26
Medical Genetics   Research Article

Phylogeography of pink pineapple mealybugs, Dysmicoccus brevipes (Cockerell) reveals the history of pineapple introduction and cultivation in China

Authors: Y.B. He, R.L. Zhan, G.M. Sun, J.B. Wu and Y.L. Zhao

The pink pineapple mealybug (PPM), Dysmicoccus brevipes (Cockerell) (Hemiptera: Pseudococcidae), is a widespread plant-sucking insect of considerable concern because it transmits the pineapple mealybug wilt-associated virus. Its distribution is closely linked with its host, the pineapple [Ananas comosus (L.) Merrill] because of its wingless and parthenoge.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.23
DOI:
10.4238/2015.August.19.23
Plant Genetics   Research Article

Study of the genetic diversity and structure of a natural population of Nectandra megapotamica (Spreng.) Mez. using RAPD markers

Authors: L.S. Costa, L.R.S. Reiniger, B.M. Heinzmann, L.P. Amaral and C.M.L. Serrote

Nectandra megapotamica (Spreng.) Mez. is a tree species that naturally occurs in the Atlantic Forest, Brazil. The aim of this study was to evaluate the genetic diversity and structure of a natural population of 12 N. megapotamica individuals using random amplified polymorphic DNA markers. Eleven primers were used in this study, producing 81 bands, of whic.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.28
DOI:
10.4238/2015.December.23.28
Microbial Genetics   Research Article

Expression of recombinant myostatin propeptide pPIC9K-Msp plasmid in Pichia pastoris

Authors: W. Du, J. Xia, Y. Zhang, M.J. Liu, H.B. Li, X.M. Yan, J.S. Zhang, N. Li, Z.Y. Zhou and W.Z. Xie

Myostatin propeptide can inhibit the biological activity of myostatin protein and promote muscle growth. To express myostatin propeptide in vitro with a higher biological activity, we performed codon optimization on the sheep myostatin propeptide gene sequence, and mutated aspartic acid-76 to alanine based on the codon usage bias of Pichia pastoris and th.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.29
DOI:
10.4238/2015.December.23.29
Plant Genetics   Research Article

Genetic relatedness of artichoke (Cynara scolymus L.) hybrids using random amplified polymorphic DNA (RAPD) fingerprinting

Authors: M.A. Sharaf-Eldin, A. Al-Tamimi, P. Alam, S.F. Elkholy and J.R. Jordan

The artichoke (Cynara scolymus L.) is an important food and medicinal crop that is cultivated in Mediterranean countries. Morphological characteristics, such as head shape and diameter, leaf shape, and bract shape, are mainly affected by environmental conditions. A molecular marker approach was used to analyze the degree of polymorphism between artichoke .. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.31
DOI:
10.4238/2015.December.23.31
Medical Genetics   Research Article

Superiority of artificial neural networks for a genetic classification procedure

Authors: I.C. Sant�Anna, R.S. Tomaz, G.N. Silva, M. Nascimento, L.L. Bhering and C.D. Cruz

The correct classification of individuals is extremely important for the preservation of genetic variability and for maximization of yield in breeding programs using phenotypic traits and genetic markers. The Fisher and Anderson discriminant functions are commonly used multivariate statistical techniques for these situations, which allow for the allocatio.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.24
DOI:
10.4238/2015.August.19.24
Plant Genetics   Research Article

Selection of reference genes for expression analyses of red-fleshed sweet orange (Citrus sinensis)

Authors: T.T. Pinheiro, D.S. Nishimura, F.B. De Nadai, A. Figueira and R.R. Latado

Red-fleshed oranges (Citrus sinensis) contain high levels of carotenoids and lycopene. The growing consumer demand for products with health benefits has increased interest in these types of Citrus cultivars as a potential source of nutraceuticals. However, little is known about the physiology of these cultivars under Brazilian conditions. Transcriptome an.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.32
DOI:
10.4238/2015.December.23.32