De novo assembly, functional annotation, and marker development of Asian pear (Pyrus pyrifolia) fruit transcriptome through massively parallel sequencing
Authors: J.F. Li, Z. Gao, Y.S. Lou, M. Luo, S.R. Song, W.P. Xu, S.P. Wang and C.X. Zhang
This study investigated the Asian pear transcriptome using the RNA-Seq normalized fruit cDNA library to create a transcriptomic resource for unigene and marker discovery. Following the removal of lowquality reads, 127,085,054 trimmed reads were assembled de novo to yield 37,649 non-redundant unigenes with an average length of 599 bp. Alternative splicing .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.22
- DOI:
- 10.4238/2015.December.23.22
Genetic variability in agronomic traits of a germplasm collection of hulless barley
Authors: X.Q. Zeng
Germplasm collections represent an important genetic source for crop improvements. In this study, 220 accessions of hulless barley were collected worldwide and their genetic diversity was investigated. Sixteen agronomic traits, including yield and yield components, grain morphology, leaf size, plant height, and lodging resistance, were assessed under fiel.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.23
- DOI:
- 10.4238/2015.December.23.23
Comparison of high-intensity focused ultrasound therapy under nasal endoscopy guidance versus first-line drug treatment in patients with persistent allergic rhinitis
Authors: G.F. Feng, Z.L. Han, F. Wang, B.C. Sun, Z.Y. Dai, S.Z. Yang and C.Y. Zhou
The aim of this study was to determine whether high-intensity focused ultrasound (HIFU) therapy under nasal endoscopy guidance could provide better efficacy and safety in patients with persistent allergic rhinitis (PAR) than the first-line drugs recommended by the World Health Organization. A total of 120 adult patients with PAR were randomly divided into.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.20
- DOI:
- 10.4238/2015.August.19.20
Heat shock protein 70 polymorphisms in Chinese patients with Graves� disease
Authors: Y.P. Wang, Z. Tang, B.K. Peng,Q. Zhen, S.L. Zhou and X.F. Jin
Graves’ disease (GD) is a common autoimmune disease mainly affecting the thyroid. However, the correlation between the development of GD and HSP70 alleles has not been reported in the Chinese population. Therefore, the aim of this study was to investigate the association between HSP70 polymorphisms and GD in the Chinese population. A total of 153 pa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.25
- DOI:
- 10.4238/2015.December.23.25
Quantitative trait locus analysis of grape weight and soluble solid content
Authors: Y.H. Zhao, Y.S. Guo, H. Lin, Z.D. Liu, H.F. Ma, X.W. Guo, K. Li, X.X. Yang, Z.Z. Niu and G.G. Shi
A grapevine hybrid population was derived from a crossing of the early-maturing female parent cultivar ‘87-1’ and the late-maturing male parent cultivar ‘9-22’. A total of 149 plants were selected from the hybrid population as the mapping population, and after sequence-related amplified polymorphism and simple-sequence repeat marke.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.21
- DOI:
- 10.4238/2015.August.19.21
Segregation analysis of microsatellite (SSR) markers in sugarcane polyploids
Authors: X. Lu, H. Zhou, Y.-B. Pan, C.Y. Chen, J.R. Zhu, P.H. Chen, Y.-R. Li, Q. Cai and R.K. Chen
No information is available on segregation analysis of DNA markers involving both pollen and self-progeny. Therefore, we used capillary electrophoresis- and fluorescence-based DNA fingerprinting together with single pollen collection and polymerase chain reaction (PCR) to investigate simple sequence repeat (SSR) marker segregation among 964 single pollens.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.26
- DOI:
- 10.4238/2015.December.23.26
Phylogeography of pink pineapple mealybugs, Dysmicoccus brevipes (Cockerell) reveals the history of pineapple introduction and cultivation in China
Authors: Y.B. He, R.L. Zhan, G.M. Sun, J.B. Wu and Y.L. Zhao
The pink pineapple mealybug (PPM), Dysmicoccus brevipes (Cockerell) (Hemiptera: Pseudococcidae), is a widespread plant-sucking insect of considerable concern because it transmits the pineapple mealybug wilt-associated virus. Its distribution is closely linked with its host, the pineapple [Ananas comosus (L.) Merrill] because of its wingless and parthenoge.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.23
- DOI:
- 10.4238/2015.August.19.23
Study of the genetic diversity and structure of a natural population of Nectandra megapotamica (Spreng.) Mez. using RAPD markers
Authors: L.S. Costa, L.R.S. Reiniger, B.M. Heinzmann, L.P. Amaral and C.M.L. Serrote
Nectandra megapotamica (Spreng.) Mez. is a tree species that naturally occurs in the Atlantic Forest, Brazil. The aim of this study was to evaluate the genetic diversity and structure of a natural population of 12 N. megapotamica individuals using random amplified polymorphic DNA markers. Eleven primers were used in this study, producing 81 bands, of whic.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.28
- DOI:
- 10.4238/2015.December.23.28
Expression of recombinant myostatin propeptide pPIC9K-Msp plasmid in Pichia pastoris
Authors: W. Du, J. Xia, Y. Zhang, M.J. Liu, H.B. Li, X.M. Yan, J.S. Zhang, N. Li, Z.Y. Zhou and W.Z. Xie
Myostatin propeptide can inhibit the biological activity of myostatin protein and promote muscle growth. To express myostatin propeptide in vitro with a higher biological activity, we performed codon optimization on the sheep myostatin propeptide gene sequence, and mutated aspartic acid-76 to alanine based on the codon usage bias of Pichia pastoris and th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.29
- DOI:
- 10.4238/2015.December.23.29
Genetic relatedness of artichoke (Cynara scolymus L.) hybrids using random amplified polymorphic DNA (RAPD) fingerprinting
Authors: M.A. Sharaf-Eldin, A. Al-Tamimi, P. Alam, S.F. Elkholy and J.R. Jordan
The artichoke (Cynara scolymus L.) is an important food and medicinal crop that is cultivated in Mediterranean countries. Morphological characteristics, such as head shape and diameter, leaf shape, and bract shape, are mainly affected by environmental conditions. A molecular marker approach was used to analyze the degree of polymorphism between artichoke .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.31
- DOI:
- 10.4238/2015.December.23.31