No difference of complete or incomplete left-sided malignant colonic obstruction on both short- and long-term outcomes
Authors: Z.-H. Liu, C. Li, N.-Q. Huang*, M.-J. Huang, H. Peng, L. Kang
The impact of complete and incomplete colonic obstruction on the short- and long-term outcomes of malignant colorectal cancer has not yet been elucidated. The aim of this study was to investigate whether there was a difference in the impacts of the 2 types of obstruction on the short- and long-term outcomes of colorectal resec.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.29.10
- DOI:
- 10.4238/2014.September.29.10
Identification and isolation of full-length cDNA sequences by sequencing and analysis of expressed sequence tags from guarana (Paullinia cupana)
Authors: L.C. Figueir�ªdo, A.C. Faria-Campos, S. Astolfi-Filho and J.L. Azevedo
The current intense production of biological data, generated by sequencing techniques, has created an ever-growing volume of unanalyzed data. We reevaluated data produced by the guarana (Paullinia cupana) transcriptome sequencing project to identify cDNA clones with complete coding sequences (full-length clones) and complete s.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1124
- DOI:
- 10.4238/vol10-2gmr1124
Homology-based cloning and expression analysis of Rf genes encoding PPR-containing proteins in tobacco
Authors: A.M. Ding, F.X. Li, Y.Q. Chen,, P. Zong, X. Qu, D.P. Gong, G.S. Liu and Y.H. Sun
As a model plant, mechanisms of the cytoplasmic male sterility/restoration of fertility (CMS/Rf) system in tobacco are seldom studied. Using Rf gene sequences from other Solanaceae plants and the draft genome of Nicotiana benthamiana, degenerate primers were designed to amplify the cDNA pool of N. tomentosiformis. In total, si.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.31.11
- DOI:
- 10.4238/2014.March.31.11
Genetic and morphological diversity of Moenkhausia oligolepis (Characiformes: Characidae) populations in the tributaries of the Araguaia River, Brazil: implications for taxonomy and conservation
Authors: T.J. Domingos, L.N. Moraes, R.M. Moresco, V.P. Margarido and P.C. Venere
Molecular genetic assessments that consider ecological information, in addition to endogamy levels, genetic diversity, and the genetic differentiation among species and populations, are particularly important for the conservation of biological diversity. Prime candidates for conservation genetic review are those subject to hum.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.29.11
- DOI:
- 10.4238/2014.September.29.11
Association study between matrix metalloproteinase-9 gene (MMP9) polymorphisms and the risk of Henoch-Sch�¶nlein purpura in children
Authors: E.D. Xu, Y.F. Xiao, J.J. Wang and L. Dong
Henoch-Schönlein purpura nephritis (HSPN), the most serious long-term complication of Henoch-Schönlein purpura, is one of the most common renal diseases in children. Matrix metalloproteinase-9 (MMP-9) is implicated in the pathogenesis of renal diseases. Genomic DNA was isolated from the venous blood leukocytes of 220.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028095
- DOI:
- 10.4238/gmr.15028095
Development of universal primers for isolating fragments of the LEAFY gene
Authors: L.X. Yu and B. Yan
LEAFY orthologs are found throughout land plants. Degenerate primers were designed for amplifying exon 3 region fragments of the LEAFY gene. Sixteen taxa from different plants were sampled, all of which were successfully isolated, which indicated that this set of primers is universally applicable for all land plants. With the use of this method, there may.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.6.1
- DOI:
- 10.4238/2013.June.6.1
Study of human acellular amniotic membrane loading bone marrow mesenchymal stem cells in repair of articular cartilage defect in rabbits
Authors: P.-F. Liu, L. Guo, D.-W. Zhao, Z.-J. Zhang, K. Kang, R.-P. Zhu and X.-L. Yuan
The aim of this study was to investigate the repair effect of human acellular amniotic membrane (HAAM) loading bone marrow mesenchymal stem cells (BMSCs) on articular cartilage defect in rabbits. Rabbit BMSCs were isolated and cultured, and they were then inoculated on HAAM to prepare the complex of HAAM and BMSCs. Twenty-four.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.29.12
- DOI:
- 10.4238/2014.September.29.12
Relationship between estrogen receptor 1 gene polymorphisms and postmenopausal osteoporosis of the spine in Chinese women
Authors: D.P. Shang, H.Y. Lian, D.P. Fu, J. Wu, S.S. Hou and J.M. Lu
The purpose of this study was to evaluate single nucleotide polymorphism (SNP) variants of the estrogen receptor 1 gene (ESR1) at rs2234693 and rs9340799, as well as to investigate the relationship between ESR gene polymorphisms and postmenopausal osteoporosis (OP) of the spine in Chinese women. We recruited 198 postmenopausal.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028106
- DOI:
- 10.4238/gmr.15028106
Active vitamin D3, 1,25-(OH)2D3, protects against macrovasculopathy in a rat model of type 2 diabetes mellitus
Authors: R. Ma, X.L. Deng, G.L. Du, C. Li, S. Xiao, Y. Aibibai and J. Zhu
To investigate the protective effect of the active form of vitamin D3, 1,25-(OH)2D3, on macrovasculopathy in rats with type 2 diabetes mellitus (T2DM), 8-week-old male Sprague-Dawley rats were randomly divided into control group, T2DM group, and treatment group. The T2DM model was established after 6 weeks by administering an .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028113
- DOI:
- 10.4238/gmr.15028113
Expression of ezrin and moesin related to invasion, metastasis and prognosis of laryngeal squamous cell carcinoma
Authors: X. Wang, M. Liu and C.Y. Zhao
We examined the expression of ezrin and moesin in laryngeal squamous cell carcinoma (LSCC) and their correlation with patient clinicopathological characteristics and overall survival. Immunohistochemical staining and reverse transcription-polymerase chain reaction (RT-PCR) for ezrin and moesin were applied to 60 carcinoma tissues, adjacent normal tissues,.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.29.13
- DOI:
- 10.4238/2014.September.29.13