Polymorphisms of the myostatin gene (MSTN) and its relationship with growth traits in goat breeds
Authors: Z.J. Zhang, Y.H. Ling, L.J. Wang, Y.F. Hang, X.F. Guo, Y.H. Zhang, J.P. Dingand X.R. Zhang
Mutations in the myostatin (MSTN) gene can inactivate its expression and result in a non-functional protein, which leads to dramatic muscularity and a “double-muscling” phenomenon in many species. Using gene sequencing and polymerase chain reaction-single-strand conformation polymorphism methods, polymorphisms of the MSTN gene were investigate.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.12
- DOI:
- 10.4238/2013.April.2.12
BMP6 and BMP4 expression in patients with cancer-related anemia and its relationship with hepcidin and s-HJV
Authors: Y.J. Shi and X.T. Pan
In the present study, we investigated BMP6 and BMP4 expression in patients with cancer-related anemia (CRA) as well as its relationship with hepcidin and s-HJV. The avidin-biotin system enzyme-linked immunosorbent assay was used to test serum levels of BMP6, BMP4, s-HJV, and hepcidin in 53 cancer patients with anemia and 52 control cancer patients without.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017130
- DOI:
- 10.4238/gmr.15017130
Genetic dissection of agronomic traits within a segregating population of breeding table grapes
Authors: A.P. Viana, S. Riaz and M.A. Walker
Grapes (Vitis vinifera) are of great economic importance worldwide. We genetically dissected a table grape breeding population, using hidden Markov models (HMM) applied to quantitative trait locus (QTL) analyses. We evaluated and dissected the following traits: total number of clusters, leaf score, peduncle length, cluster length, number of berries, weigh.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.11
- DOI:
- 10.4238/2013.April.2.11
In silico analysis of diverse endophytic fungi by using ITS1-5,8S-ITS2 sequences with isolates from various plant families in Brazil
Authors: S.A. Rhoden, A. Garcia, J.L. Azevedo and J.A. Pamphile
Brazil has a great diversity of plants, and considering that all plant species studied to date have endophytic microorganisms (bacteria or fungi), the country is a resource in the search for bioactive compounds. Endophytes live within plants without causing damage and may be in dynamic equilibrium with the health of the plant. Endophytic fungi can be iden.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.10
- DOI:
- 10.4238/2013.April.2.10
Prevalence of human papillomavirus genotypes among women with cervical lesions in the Shaanxi Province of China
Authors: J. Li, Y.Y. Wang, X. Nan, X.F. Tian, T. Yan, P. Wang, Y. Yin, Y. Liu, R. Yuan, G.Q. Wang and Y.L. Fu
This study aimed to investigate human papilloma virus (HPV) genotypes among women with cervical lesions in Shaanxi Province, China, to obtain information regarding cervical lesion prevention and treatment. The study included 4508 HPV-positive subjects; cervical swab specimens were collected and tested for HPV infection status and HPV genotypes using polym.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017181
- DOI:
- 10.4238/gmr.15017181
Complementary DNA cloning, sequence analysis, and tissue transcription profile of a novel U2AF2 gene from the Chinese Banna mini-pig inbred line
Authors: S.Y. Wang, J.L. Huo, Y.W. Miao, W.M. Cheng and Y.Z. Zeng
U2 small nuclear RNA auxiliary factor 2 (U2AF2) is an important gene for pre-messenger RNA splicing in higher eukaryotes. In this study, the Banna mini-pig inbred line (BMI) U2AF2 coding sequence (CDS) was cloned, sequenced, and characterized. The U2AF2 complete CDS was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) technique.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.9
- DOI:
- 10.4238/2013.April.2.9
Antimicrobial activity and cytotoxicity of endophytes from Scapania verrucosa Heeg.
Authors: J.-G. Wu, W. Peng, P.-Y. Zeng, Y.-B. Wu1, J. Yi and J.-Z. Wu
We evaluated the antibacterial activity and cytotoxicity of endophytes isolated from Scapania verrucosa Heeg., which belongs to the liverwort class. A total of 49 endophytic fungi were isolated from S. verrucosa and classified into seven genera and one family in our previous study. In this study, the cytotoxic activity of the endophytes was assessed using.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.8
- DOI:
- 10.4238/2013.April.2.8
cDNA cloning and characterization of two trehalases from Spodoptera litura (Lepidoptera; Noctuidade)
Authors: Q. Zou, P. Wei, Q. Xu, H.Z. Zheng, B. Tang and S.G. Wang
The oriental leafworm moth, Spodoptera litura, is a major agricultural pest in southeast Asia and nearby Pacific regions. Two distinct trehalases have been identified in insects: soluble trehalase (Treh1) and membrane-bound trehalase (Treh2), although there is currently no information on these genes in S. litura. To characterize the distribution and funct.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.7
- DOI:
- 10.4238/2013.April.2.7
Population structure of the blood clam (Tegillarca granosa) in China based on microsatellite markers
Authors: Y.J. Wang, Q.G. Zeng and L.N. Xu
The blood clam, Tegillarca granosa, is widely cultivated in China. We isolated 6 microsatellite loci from T. granosa and used them to investigate genetic diversity and population structure of 5 widely distributed populations of blood clam collected from eastern and southeastern China. The allele number per locus varied from 4 to 9, and the polymorphism in.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.6
- DOI:
- 10.4238/2013.April.2.6
Genetic variability in Blanco Orejinegro breed cattle populations in Colombia
Authors: R. MartÃ?Ânez, A.P. LlinÃ?¡s and J.F. M.-Rocha
We analyzed population structure and genetic diversity in Blanco Orejinegro Creole cattle with 12 microsatellite markers, genotyping 138 individuals belonging to 7 commercial and 3 conservation herds. These markers showed a high level of polymorphism; 171 alleles were identified. The mean number of alleles per locus was 5.63 (3.82-6.58). The total number .. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.4
- DOI:
- 10.4238/2013.April.10.4