Polymorphism in the melatonin receptor gene in buffalo populations of the Brazilian Amazon
Authors: E.B. Machado, B.B. Souza, R.C. Guimar�£es, J.S.N. Azevedo, E.C. Gon�§alves, H.F.L. Ribeiro, S.T. Rolim Filho, E. Silva Filho
Buffalo farming in Brazil is increasing, as is the challenge of identifying molecular markers that will improve productivity. Therefore, the aim of this study was to analyze single nucleotide polymorphisms of the receptor gene for the hormone melatonin in buffaloes from northern Brazil by polymerase chain reactions (PCRs) and .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027960
- DOI:
- 10.4238/gmr.15027960
DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA
Authors: S. Tabasum, F.A. Khan, S. Nawaz, M.Z. Iqbal and A. Saeed
DNA profiles of 40 sugarcane genotypes were constructed with 30 RAPD markers. Sugarcane genotypes of both Saccharum officinarum and S. barberi were included in this study. Multiple alleles were detected from each RAPD; there was a high level of polymorphism. On average, 7.93 alleles were produced per primer, giving a total of .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr709
- DOI:
- 10.4238/vol9-1gmr709
Analysis of interleukin 19 serum levels and single nucleotide polymorphisms in systemic lupus erythematosus
Authors: J.R. Lin, H.H. Qin, Y. Wang, J. Liang and J.H. Xu
Systemic lupus erythematosus (SLE) is an autoimmune connective tissue disease that affects multiple organs and diminishes a patients’ quality of life. It has been suggested that interleukin 19 (IL-19) is engaged in intercellular signal transduction, which is related to the immune response and the local inflammatory react.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028007
- DOI:
- 10.4238/gmr.15028007
Conserved baculoviral ORFs 10 and 14 from Bombyx mori multiple nucleopolyhedrovirus
Authors: S.A. Santos, J.L.C. Silva, V.A. Balani, F.A.V. Seixas and M.A. Fernandez
ORFs 10 and 14 from Bombyx mori multiple nucleopolyhedrovirus (BmMNPV) were amplified, cloned and sequenced. Nucleotide analysis of these genes and those of other baculoviruses showed that these genes are highly conserved. The p10 protein from BmMNPV ORF10 has 70 amino acid residues similar to that of the four other known BmNP.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr723
- DOI:
- 10.4238/vol9-1gmr723
Cytotoxic and immunomodulatory effects of Ebenus boissieri Barbey on breast cancer cells
Authors: N. Imir, E. Aydemir E. Simsek, R.S. Gokturk, E. Yesilada and K. Fiskin
We aimed to determine the cytotoxic and immunomodulatory effects of hydroalcoholic extracts of the roots and aerial parts of Ebenus boissieri (EB) on breast cancer MDA-MB231 cells and the non-cancerous human embryonic kidney cell line, 293T. Cell viability was determined by MTT assay, trypan blue exclusion, and Live/Dead Viabi.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017766
- DOI:
- 10.4238/gmr.15017766
Genetic diversity analysis of Aspergillus flavus isolates from plants and air by ISSR markers
Authors: M.A. Mahmoud, A.M.A. El-Samawaty, M.A. Yassin and A.R.M. Abd El-Aziz
Aspergillus flavus is one of the most abundant and widely distributed fungi on earth. A. flavus produces aflatoxins (AFs), which are toxic secondary metabolites. AFs have harmful effects on public health (humans and animals) and agricultural crops. Inter-simple sequence repeat (ISSR) markers were used to analyze the genetic di.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028081
- DOI:
- 10.4238/gmr.15028081
Role of IL-10 polymorphisms in susceptibility to hepatitis B virus-related hepatocellular carcinoma
Authors: M.W. Peng, S.Q. Lu, J. Liu and C.Y. Dong
We conducted a case-control study to investigate the role of three common single nucleotide polymorphisms of IL-10 (-592G/A, -819T/C, and -1082A/C) in the development of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). The study included 173 HBV-related HCC patients and 182 healthy controls. A polymerase chain r.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017984
- DOI:
- 10.4238/gmr.15017984
Monitoring tissue blood oxygen saturation in the internal jugular venous area using near infrared spectroscopy
Authors: Z.S. Ruan, T. Li, R.R. Ren, Y. Zhao, K. Li, Y.F. Mao, G. Shen and L. Jiang
Central venous blood oxygen saturation (ScvO2) is an important monitoring index of fluid resuscitation. However, monitoring of ScvO2 is not continuous and invasive. Near infrared spectroscopy (NIRS) is an optical technology for the noninvasive detection of hemodynamic changes, with advantages of being real-time, continuous, lo.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.31.23
- DOI:
- 10.4238/2015.March.31.23
Selection and use of reference genes in mouse mammary glands
Authors: L.Q. Han, G.Y. Yang, H.S. Zhu, Y.Y. Wang, L.F. Wang, Y.J. Guo, W.F. Lu, H.J. Li and Y.L. Wang
Obtaining quantitative data concerning gene expression is important for understanding milk synthesis in mammary glands. Quantitative real-time PCR (qRT-PCR) is an efficient tool to calculate gene expression; however, it is necessary to find valid reference genes for normalization of qRT-PCR data. We applied the geNorm software.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr724
- DOI:
- 10.4238/vol9-1gmr724
Relationships among lipid peroxidation, SOD enzyme activity, and SOD gene expression profile in Lycopersicum esculentum L. exposed to cold stress
Authors: S. Soydam Aydin, �°. B�¼y�¼k and S. Aras
The current study was designed to evaluate lipid peroxidation (via malondialdehyde) levels, the superoxide dismutase (SOD) gene expression profile, and SOD enzyme activity in tomato plants (Lycopersicum esculentum L.) subjected to different time periods of cold stress (control, 2, 4, 6, 8, and 10 days). Results revealed that maximum lipid peroxidation occ.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.29.6
- DOI:
- 10.4238/2013.August.29.6