Expression analysis of differentially expressed miRNAs in male and female chicken embryos
Authors: Y.P. Feng, J.F. Chen, P. Huang, X. Wang, J. Wang, X.L. Peng and Y.Z. Gong
MicroRNAs (miRNAs) are small non-coding RNA molecules that play key roles in the regulation of development processes of many tissues and organs at the post-transcriptional level. However, little is known about how they affect chicken gonadal development. We examined the expression of four miRNAs (miR-218, -200b, -196, and -206.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.17.2
- DOI:
- 10.4238/2014.April.17.2
Identification of a major quantitative trait locus for ear size induced by space flight in sweet corn
Authors: Y.T. Yu, G.K. Li, Z.L. Yang, J.G. Hu, J.R. Zheng and X.T. Qi
The development of molecular markers has contributed to progress in identifying the gene(s) responsible for favorable variations in maize studies. In this study, quantitative trait locus (QTL) mapping was conducted using simple sequence repeat markers in an F2 sweet corn population from a cross between parental line 1132 and s.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.17.3
- DOI:
- 10.4238/2014.April.17.3
Genetic association of catechol-O-methyltransferase val(158)met polymorphism in Saudi schizophrenia patients
Authors: S. Al-Asmary, S. Kadasah, M. Arfin, M. Tariq and A. Al-Asmari
Schizophrenia is a complex neuropsychiatric disorder strongly associated with dopamine dysregulation. Catechol-O-methyl-transferase (COMT) is a candidate gene for schizophrenia that encodes an enzyme involved in the metabolic inactivation of dopamine. The COMT Val158Met polymorphism has been associated with schizophrenia and h.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.17.4
- DOI:
- 10.4238/2014.April.17.4
Proteomic response of Moniliophthora perniciosa exposed to pathogenesis-related protein-10 from Theobroma cacao
Authors: F.A.C. Silva1, C.P. Pirovani1, S. Menezes1, C. Pungartnik2, A.S. Santiago1, M.G.C. Costa1, F. Micheli3 and A.S. Gesteira4
TcPR-10, a member of the pathogenesis-related protein 10 family, was identified in EST library of interactions between Theobroma cacao and Moniliophthora perniciosa. TcPR-10 has been shown to have antifungal and ribonuclease activities in vitro. This study aimed to identify proteins that are differentially expressed in M. perniciosa in response to TcPR-10.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.22.5
- DOI:
- 10.4238/2013.October.22.5
Molecular detection of QTL controlling plant height components in a doubled haploid barley population
Authors: X.F. Ren, D.F. Sun, W.B. Dong, G.L. Sun and C.D. Li
Yield losses caused by lodging in barley can be partially controlled by reducing plant height. In order to understand dwarfing mechanisms and efficiently use new dwarf germplasms for a breeding program, it is important to identify QTL of plant height components. QTL analysis was performed for seven plant height component traits using a DH population of 12.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.17.5
- DOI:
- 10.4238/2014.April.17.5
Role of XRCC1 and ERCC5 polymorphisms on clinical outcomes in advanced non-small cell lung cancer
Authors: D. Liu, J. Wu, G.Y. Shi, H.F. Zhou and Y. Yu
We aimed to assess the role of polymorphisms of the XRCC1 Arg194Trp, XRCC1 Arg399Gln, ERCC5 His1104Asp, and ERCC5 His46His genes on clinical outcomes of advanced non-small cell lung cancer (NSCLC) patients receiving platinum-based chemotherapy regimens. A total of 378 NSCLC patients were asked to participate within 1 month aft.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.17.6
- DOI:
- 10.4238/2014.April.17.6
Comparison of the sensitivity of strains of Salmonella enterica serovar Typhimurium in the detection of mutagenicity induced by nitroarenes
Authors: C.R. Rainho, S.M. Corr�?ªa, J.L. Mazzei, C.A.F. Aiub and I. Felzenszwalb
The use of strains of Salmonella enterica serovar Typhimurium with different metabolic capacities can indicate the class or classes of compounds present in an environmental sample and enable the diagnosis of the mutagenic activity of these pollutants adsorbed on particulate matter (PM) in the air. In the present study, the sen.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.9
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.9
Rapid method for DNA extraction from the honey bee Apis mellifera and the parasitic bee mite Varroa destructor using lysis buffer and proteinase K
Authors: M.R.C. Issa1, V.L.C. Figueiredo2, D. De Jong1, C.H. Sakamoto1 and Z.L.P. Sim�?µes2
We developed a rapid method for extraction of DNA from honey bees, Apis mellifera, and from the parasitic bee mite, Varroa destructor. The advantages include fast processing and low toxicity of the substances that are utilized. We used lysis buffer with nonionic detergents to lyse cell walls and proteinase K to digest proteins. We tested whole thorax, tho.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.22.4
- DOI:
- 10.4238/2013.October.22.4
Clinical features of diabetes mellitus cases complicated by Burkholderia pseudomallei septicemia
Authors: H.B. Quan, T.Y. Li, Y.Y. Gao and D.X. Chen
The aim of this study was to analyze the clinical characteristics of diabetes mellitus patients with Burkholderia pseudomallei septicemia and evaluate strategies of diagnosis and treatment. The clinical characteristics, diagnosis, treatment, and prognosis of 39 diabetes mellitus patients with B. pseudomallei septicemia were re.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.17.7
- DOI:
- 10.4238/2014.April.17.7
A duplex SYBR Green I real-time quantitative PCR assay for detecting Escherichia coli O157:H7
Authors: X. Yang*, H.W. Cheng*, L. Chen, J. Zhao, H.T. Chang, X.W. Wang, H.Y. Liu, H.X. Yao, L.X. Zhang and C.Q. Wang
PCR and hybridization assays are widely used for the detection and identification of Escherichia coli serogroups and serotypes. We used these techniques for the detection of E. coli O157:H7, a dominant serogroup among E. coli strains that are considered major public health problems worldwide. We developed a quantitative PCR assay using SYBR Green I, based.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.22.3
- DOI:
- 10.4238/2013.October.22.3