An enhancer/promoter combination strengthens the expression of blood-coagulation factor VIII in non-viral expression vectors
Authors: V. Pican�§o-Castro, E.M.S. Russo-Carbolante, A.M. Fontes,A.C. Fernandes and D.T. Covas
We explored the potential of fusion of hepatic locus control region 1 (HCR-1) with HCR-2 to express B-domain-deleted human factor VIII (FVIII) in four cell lines. B-domain-deleted human FVIII expression was controlled by HCR-1/HCR-2, followed by liver specific and ubiquitous promoters. Chimera enhancer HCR-1/HCR-2, followed by.. Read More»
- Genet. Mol. Res. 7(2):
- vol7-2gmr447
- DOI:
- 10.4238/vol7-2gmr447
Molecular cloning and functional characterization of a glutathione S-transferase involved in both anthocyanin and proanthocyanidin accumulation in Camelina sativa (Brassicaceae)
Authors: Y. Wang, Y. Tang, M. Zhang, F. Cai, J. Qin, Q. Wang, C. Liu, G. Wang, L. Xu, L. Yang, J. Li, Z. Wang and X. Li
Recently, we found that the Arabidopsis TT19 protein, a glutathione S-transferase, has two functional domains that influence both anthocyanin and proanthocyanidin accumulation. To further understand the function of this protein in the other species, we cloned a cDNA encoding a glutathione S-transferase (namely CMGSTF12) from Camelina sativa, an oil crop t.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.25.4
- DOI:
- 10.4238/2012.September.25.4
Validation of two real-time PCRs targeting the PE-PGRS 20 gene and the region of difference 4 for the characterization of Mycobacterium bovis isolates
Authors: M.L. Sales, A.A. Fonseca Jr., L. Orzil, A.P. Alencar, M.A. Hodon, M.A. Issa, P.M. Soares Filho, M.R. Silva, A.P. Lage and M.B. Heinemann
This study aimed to develop and validate real-time PCR for the diagnosis of Mycobacterium bovis isolates. Two hundred and seventy-four M. bovis isolates and 156 M. tuberculosis isolates were tested. Both qPCRs amplified all of the 274 M. bovis samples, but none of the 156 M. tuberculosis samples. The qPCR for PE-PGRS 20 had 91.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.18.3
- DOI:
- http://dx.doi.org/10.4238/2014.June.18.3
Development of SNP-based dCAPS markers for identifying male sterile gene tms5 in two-line hybrid rice
Authors: F.S. Song, J.L. Ni1, Y.L. Qian, L. Li, D.H. Ni and J.B. Yang
Molecular markers can increase both the efficiency and speed of breeding programs. Functional markers that detect the functional mutations causing phenotypic changes offer a precise method for genetic identification. In this study, we used newly derived cleaved amplified polymorphic sequence markers to detect the functional mu.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038512
- DOI:
- 10.4238/gmr.15038512
Detection of Jaagsiekte sheep retrovirus in the peripheral blood during the pre-clinical period of ovine pulmonary adenomatosis
Authors: Y. Liu, Y.F. Zhang, X.L. Sun and S.Y. Liu
The envelope protein (Env) of the Jaagsiekte sheep retrovirus (JSRV) is known to be a unique oncoprotein responsible for inducing ovine pulmonary adenocarcinoma (OPA). The objective of this study was to prepare a specific monoclonal antibody (mAb) against the JSRV Env protein using bioinformatic analysis. According to the stru.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038521
- DOI:
- 10.4238/gmr.15038521
Cloning of an anthocyanidin synthase gene homolog from blackcurrant (Ribes nigrum L.) and its expression at different fruit stages
Authors: X.-G. Li, J. Wang and Z.-Y. Yu
Anthocyanidin synthase (ANS), a 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase, catalyzes the penultimate step in anthocyanin biosynthesis, from leucoanthocyanidins to anthocyanidins, the first colored compound in the anthocyanin pathway. In this study, a full-length, 1427-bp long cDNA named RnANS1, which is homologous to the anthocyanidin synthase g.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.31.2
- DOI:
- http://dx.doi.org/10.4238/2015.March.31.2
Safety of recombinant human granulocyte-macrophage colony-stimulating factor in healing pediatric severe burns
Authors: Y.F. Chi, J.K. Chai, H.M. Luo, Q.X. Zhang and R. Feng
We explored the safety of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) for healing burns in children. Subjects were randomly assigned to two groups: the experimental group received external rhGM-CSF gel, and the control group received rhGM-CSF gel matrix components, applied to the burn surface. Neither group was given any .. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.31.3
- DOI:
- http://dx.doi.org/10.4238/2015.March.31.3
Twisting of the spermatic cord: ischemia and reperfusion, toxicogenetic evaluation, and the effects of phosphatidylcholine in pre-clinical trials
Authors: H.R.S. Coelho, C.R. Berno, G.R. Falc�£o, C.R. Hildebrand, R.J. Oliveira and A.C.M.B. Antoniolli-Silva
Phosphatidylcholine is the main phospholipid present in cell membranes and in lipoproteins, and can interfere with various biological processes. This lipid also has antioxidant activity, and protects against damage caused by free radicals under conditions of ischemia/reperfusion. Therefore, the present study was designed toeva.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038529
- DOI:
- 10.4238/gmr.15038529
Immunological function and antibacterial activity of two ferritin proteins from the freshwater pearl mussel Hyriopsis schlegelii
Authors: J.Q. Sheng, Q.C. Shu, J.W. Shi, J.H. Wang, K. Peng, S. Yuan and Y.J. Hong
Ferritin is a conserved iron-binding protein involved in host defense and cellular iron metabolism in most organisms. We investigated the expression profiles of two ferritin genes (designated HsFer-1 and HsFer-2) in the hemocytes, gonad, and hepatopancreas of Hyriopsis schlegelii, when challenged with bacteria and metal ions. .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038533
- DOI:
- 10.4238/gmr.15038533
Simultaneous presence of bovine papillomavirus and bovine leukemia virus in different bovine tissues: in situ hybridization and cytogenetic analysis
Authors: A. Yaguiu, M.L.Z. Dagli, E.H. Birgel Jr., B.C.A. Alves Reis,O.P. Ferraz, E.M. Pituco6, A.C. Freitas, W. Be�§ak and R.C. Stocco
Bovine papillomavirus (BPV) DNA sequences were detected in different tissues, in addition to epithelium. Cytogenetic abnormalities were observed in blood lymphocytes. The presence of more than one virus in a single tissue is a difficult aspect to evaluate,especially when the DNA sequences are detected in tissues that are not s.. Read More»
- Genet. Mol. Res. 7(2):