Inhibitory effect of microRNA-24 on fatty acid-binding protein expression on 3T3-L1 adipocyte differentiation
Authors: M. Kang1*, L.M. Yan2*, Y.M. Li3, W.Y. Zhang3, H. Wang3, A.Z. Tang1 and H.S. Ou3
We examined the effect of microRNAs on 3T3-L1 adipocyte differentiation and expression of adipocyte-specific gene fatty acid-binding protein 4 (FABP4). We screened and identified adipo-related microRNAs during 3T3-L1 adipocyte differentiation with a microRNA microarray. High expression plasmids of miR-24 and miR-21 were constructed and transfected into 3T.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.November.7.1
- DOI:
- 10.4238/2013.November.7.1
Expressions of Src homology 2 domain-containing phosphatase and its clinical significance in laryngeal carcinoma
Authors: L.B. Dong1, G.Q. Li2, Z.H. Tian2, Z.M. Wang2 and K. Xu2
We investigated the expression of Src homology 2 domain-containing phosphatase (SHP-2) in laryngeal carcinoma and its clinical significance. Expression of SHP-2 was detected by immunohistochemical staining in normal mucosal tissues and various grades of laryngeal carcinoma. We looked for possible correlations between expression of SHP-2 in laryngeal carci.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.7.6
- DOI:
- 10.4238/2013.October.7.6
Intraspecific and interspecific polyploidy of Brazilian species of the genus Inga (Leguminosae: Mimosoideae)
Authors: M.F. Figueiredo, R.L.A. Bruno, A.E. Barros e Silva, S. Nascimento, I.G. Oliveira and L.P. Felix
We investigated the karyotypes of 13 species of six sections of the genus Inga (Leguminosae-Mimosoideae) from Brazil. We used conventional Giemsa staining to identify numerical chromosomal variations and looked for karyotypic evolutionary patterns. The karyotypes generally had small chromosomes, varying from metacentric to sub.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.18
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.18
Expression of tyrosine hydroxylase and growth-associated protein 43 in aging atrial fibrillation patients of Xinjiang Uygur and Han nationality
Authors: Y.D. Li1, J.X. Li1, B.-P. Tang1, T.Y. Gan1, G.J. Xu1, X.H. Zhou1, H. Li2,X. Guo2, A. Mahemuti1, Q. Sun3, Y.Y. Zhang1 and J. Wang1
The aim of this study was to explore the changes in gene and protein expressions of tyrosine hydroxylase (TH) and growth-associated protein 43 (GAP43) in aging atrial fibrillation patients of Xinjiang Uygur and Han nationality, and the significance of the changes. Real-time polymerase chain reaction and Western blot analysis were used to detect gene and p.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.10
- DOI:
- 10.4238/2013.October.30.10
Three-dimensional digital visible heart model and myocardial pathological characteristics of fetal single ventricle connected with aortic coarctation
Authors: B. Ren1*, Y. Jiang1*, H.M. Xia1, X.Y. Li1, L.W. Tan2, Y. Li2, Q.Y. Li2,X.S. Li2 and Y.H. Gao1
This study aimed to provide data for imaging diagnosis and clinical surgical plans by reconstructing a three-dimensional (3-D) digital visible heart model of single ventricle (SV) connection with aortic coarctation (CoA) and characterizing the myocardial and vascular wall pathological characteristics. Fifteen miscarried fetus cadavers with SV and CoA were.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.9
- DOI:
- 10.4238/2013.October.30.9
Molecular characteristic and expression analysis of collagenolytic serine protease from the Chinese mitten crab Eriocheir sinensis with defense response to Vibrio anguillarum challenge
Authors: Q.Z. Yang, Z.J. Yang, Y. Zhang, X.L. Li and W. Zhang
A novel collagenolytic serine protease (CLSP) was cloned from the hemocytes of the Chinese mitten crab Eriocheir sinensis (Es-CLSP). The full-length cDNA of Es-CLSP contains 990 nucleotides. It encodes a 270-amino acid-long peptide with the mature peptide containing 221 amino acids. It contains the conserved catalytic triad (H.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.1
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.1
Differential expression of L-FABP and L-BABP between fat and lean chickens
Authors: Q. Zhang1,2, H. Shi1,2, W. Liu1,2, Y. Wang1,2, Q. Wang1,3 and H. Li1,2
Liver fatty acid-binding protein (L-FABP) and liver bile acid-binding protein (L-BABP), in the liver intra-cytoplasm of chicken, are members of the fatty acid-binding protein subfamily. This study was designed to analyze and compare L-FABP and L-BABP expression levels between fat and lean lines in chicken liver tissue, and to determine the relationship be.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.7.5
- DOI:
- 10.4238/2013.October.7.5
Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus
Authors: L.N. Sun1,2, H.S. Yang1, M.Y. Chen3 and D.X. Xu1,2
Many developmental genes play important roles in regeneration. Here, we cloned the two developmental genes, Wnt6 and Hox6, from Apostichopus japonicus by rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The Wnt6 full-length cDNA is 1140 bp long with a 1029-bp open reading frame encoding 342 amino acids. The deduced Wnt6 protein con.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.7
- DOI:
- 10.4238/2013.November.7.7
Genetic variability of Dactylopius opuntiae (Hemiptera, Dactylopiidae) on forage cactus in northeast Brazil
Authors: D.M.P. Silva1, A.C. do E.S. Mergulh�£o2, L.V. de Medeiros2,M.V.B. Figueiredo3 and H.A. Burity3
The carmine cochineal Dactylopius opuntiae is a key pest in productive fields of forage cactus in Pernambuco, Brazil. Species identification by means of molecular markers assists in understanding the genetic profile, underpins morphological characterization, and supports the monitoring of populations in integrated management programs designed to control t.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.8
- DOI:
- 10.4238/2013.October.30.8
Development of 1047 insertion-deletion markers for rice genetic studies and breeding
Authors: Y.X. Zeng*, Z.H. Wen*, L.Y. Ma, Z.J. Ji, X.M. Li and C.D. Yang
In this study, a total of 1047 insertion-deletion (InDel) primer pairs distributed across the rice genome were developed and experimentally validated. The primer pairs were designed based on the InDel length polymorphisms between 93-11 (Oryza sativa ssp indica cv.) and Nipponbare (Oryza sativa ssp japonica cv.), aiming for utilization between indica and j.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.7
- DOI:
- 10.4238/2013.October.30.7