Association of the IL-6 -174G/C gene polymorphism with knee osteoarthritis in a Thai population
Authors: S. Honsawek, B. Deepaisarnsakul, A. Tanavalee, P. Yuktanandana, P. Bumrungpanichthaworn, S. Malila and N. Saetan
Osteoarthritis is a chronic progressive degenerative joint disease characterized by age-related regressive change in articular cartilage. A single nucleotide polymorphism has been described at position -174 of the interleukin-6 (IL-6) promoter region, leading to three possible genotypes, GG, GC, and CC. We investigated a possi.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1161
- DOI:
- 10.4238/vol10-3gmr1161
Applicability of RAPD markers on silverstained polyacrylamide gels to ascertain genetic diversity in Peripatus acacioi (Peripatidae; Onychophora)
Authors: Daiane Mariele DeLaat, Maria Raquel Santos Carvalho, Maria Bernadete Lovato, Maria Dolores Porto Acedo and Cleusa Gra�§a da Fonseca
RAPD (random amplification of polymorphic DNA) molecular markers can be utilized for analyzing genetic variability in populations for which only a few or no molecular markers are available. They were used in a study of an endangered species, Peripatus acacioi, found in the Tripuí Ecological Station, in Ouro Preto, MG, B.. Read More»
- Genet. Mol. Res. 4(4):
Molecular characterization of urdbean (Vigna mungo) germplasm related to resistance against urdbean leaf crinkle virus
Authors: R. Binyamin, M. Aslam Khan, A.I. Khan, M. Azam Khan, F.S. Awan and N.A. Khan
Urdbean (Vigna mungo) is an important pulse crop grown worldwide. Urdbean leaf crinkle virus (ULCV) is a pathogen of urdbean found in Pakistan that causes huge losses in yield. Forty urdbean varieties/lines were screened against the virus under field conditions during spring season 2009. None of the lines appeared to be highly.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1446
- DOI:
- 10.4238/vol10-3gmr1446
Development and optimization of a fluorescent differential display PCR system for studying bovine embryo development in vitro
Authors: Paula Ripamonte, Giovana Krempel Merighe, Yeda Fumie Watanabe, Alexandre Rodrigues Caetano and Fl�¡vio Vieira Meirelles
Differential display is a widely used methodology to identify genes that are differentially expressed in biological samples. We developed a new protocol for the amplification and recovery of differentially expressed genes from extremely small initial amounts of RNA (10 to 25 pg mRNA) from preimplantation bovine embryos. The cD.. Read More»
- Genet. Mol. Res. 4(4):
Single nucleotide polymorphism analysis of exons 3 and 4 of IGF-1 gene in pigs
Authors: L.L. Hao, H. Yu, Y. Zhang, S.C. Sun, S.C. Liu, Y.Z. Zeng, Y.X. Ai and H.Z. Jiang
The IGF-1 gene has been implicated as a candidate gene for the regulation of pig growth traits. We analyzed exons 3 and 4 of IGF-1 gene polymorphisms of the Banna mini-pig (28), the Tibetan mini-pig (30), the Junmu pig (55), and L. Yorkshire species (50) using PCR-SSCP. Three genotypes in exon 3 and 6 genotypes in exon 4 were .. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1328
- DOI:
- 10.4238/vol10-3gmr1328
Breeding cassava for apomixis
Authors: Nagib M.A. Nassar and Rosane G. Collevatti
Apomixis genes have been successfully transferred to cassava (Manihot esculenta) by hybridizing it with the wild species, M. glaziovii. The interspecific hybrid of cassava and M. glaziovii was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were g.. Read More»
- Genet. Mol. Res. 4(4):
Isolation and characterization of 19 polymorphic microsatellite loci from the topmouth gudgeon, Pseudorasbora parva
Authors: C. Zeng, Y. Gul, K. Yang, L. Cui, W.-M. Wang and Z.-X. Gao
The Asiatic topmouth gudgeon, Pseudorasbora parva, is recognized as one of the most invasive fish species in many countries outside of Asia. We isolated and characterized 19 microsatellite loci from P. parva. The polymorphism of these 19 loci was tested on 40 individuals of P. parva sampled from a wild population located in Ez.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1477
- DOI:
- 10.4238/vol10-3gmr1477
Localization of rDNA sites in holocentric chromosomes of three species of triatomines (Heteroptera, Triatominae)
Authors: Grasiela Dias de Campos Severi-Aguiar and Maria TercÃ?Âlia Vilela de Azeredo-Oliveira
Chromatin organization in the holocentric chromosomes of three triatomines species was cytologically studied by fluorescent in situ hybridization with a 45S rDNA probe of Drosophila melanogaster to localize ribosomal genes. In Triatoma tibiamaculata, metaphases I showed telomeric highlights in a single, larger bivalent. In T. .. Read More»
- Genet. Mol. Res. 4(4):
Development and characterization of microsatellite markers for the lizardfish known as the Bombay duck, Harpadon nehereus (Synodontidae)
Authors: T.J. Xu, D.Q. Sun, H.Y. Li and R.X. Wang
The Bombay duck, or bummalo (Harpadon nehereus), is a lizardfish native to the Arabian sea, but also common in the China sea. It is normally dried and salted before consumption and export. To provide molecular information on this economically important fish species, we developed and characterized microsatellite markers. Ninety.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1045
- DOI:
- 10.4238/vol10-3gmr1045
Association among oxidized LDL levels, MnSOD, apolipoprotein E polymorphisms, and cardiovascular risk factors in a south Brazilian region population
Authors: Maria G.V. Gottlieb, Carla H.A. Schwanke, Adriana F.R. Santos, Paulo F. Jobim, Denise P. M�¼ssel and Ivana B.M. da Cruz
Oxidized LDL (ox-LDL) is involved in the initiation and progression of atherosclerosis. Many factors can affect the LDL oxidation such as oxidative stress. The present study tested whether ox-LDL levels would be associated with apolipoprotein E (APOE), manganese superoxide dismutase (MnSOD) Ala16Val polymorphisms, and classic .. Read More»
- Genet. Mol. Res. 4(4):