Isolation and characterization of 48 polymorphic microsatellite markers for the blood clam Scapharca broughtonii (Arcidae)
Authors: J.-T. Tian Z.-H. Liu L.-Q. Zhou B. Wu P. Liu A.-G. Yang
Blood clams (Scapharca broughtonii) are widely cultivated and consumed in noutheast Asia. Forty-eight polymorphic microsatellite loci were developed for this clam using magnetic-bead hybridization enrichment. The number of alleles per locus ranged from 2 to 14. Polymorphism of these loci was assessed in 30 individuals from a population collected from coas.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.11.2
- DOI:
- 10.4238/2012.October.11.2
Dephosphorylation of NSSR1 regulates alternative splicing of the GluR-B minigene
Authors: W. Zhang, L.L. Li, X.H. Chen and Z.Y. Peng
Neural salient serine/arginine-rich protein 1 (NSSR1, alternatively SRp38) is an important splicing factor that can repress pre-mRNA alternative splicing in cells during heat shock and mitosis. We show here that NSSR1 protein is dephosphorylated when cells are heat shocked or incubated with kinase inhibitor K252a. Both heat sh.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.3
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.3
Molecular cloning and sequence analysis of follicle-stimulating hormone beta polypeptide precursor cDNA from the bovine pituitary gland
Authors: L.S. Dai, Y.M. Zhao, G.L. Zhang, R.F. Zhao, H. Jiang, T.H. Ma, Y. Gao, B. Yuan, Y.L. Xu, W.Y. Yu, Z.H. Zhao and J.B. Zhang
Follicle-stimulating hormone (FSH) plays an essential role in mammalian spermatogenesis and follicular development. In a previous study, we demonstrated that some bulls carry numerous linked mutations in the FSH beta-subunit (FSHB) gene, and that these bulls have poor-quality semen, low fertility, and slightly lower serum FSH .. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr997
- DOI:
- 10.4238/vol10-3gmr997
Structure and polymorphism of 16 novel Y-STRs in Chinese Han Population
Authors: G.Q. Zhang S.Y. Yang L.L. Niu D.W. Guo
Y-chromosome short tandem repeats (Y-STRs) are useful tools for identifying paternity origin and male-female mixed samples because of their male-specificity, haploid inheritance and relatively simplicity. We focused on novel Y-STRs deposited in the human Genome database from DYS708 to DYS726. We typed 16 male-specific Y-STRs from males of a Chinese Han popul.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.11.1
- DOI:
- 10.4238/2012.October.11.1
Routine libraries for pattern recognition in quasispecies
Authors: E.A. Marucci, G.F.D. Zafalon, A.C.G. Jardim, L.H.T. Yamasaki,C. Bittar, P. Rahal and J.M. Machado
The results obtained through biological research usually need to be analyzed using computational tools, since manual analysis becomes unfeasible due to the complexity and size of these results. For instance, the study of quasispecies frequently demands the analysis of several, very lengthy sequences of nucleotides and amino ac.. Read More»
- Genet. Mol. Res. 7(3):
- vol7-3x-meeting013
- DOI:
- 10.4238/vol7-3x-meeting013
Heteroduplex formation and S1 digestion for mapping alternative splicing sites
Authors: E.N. Ferreira, M.C.R. Rangel, P.B. Pineda, D.O. Vidal, A.A. Camargo, S.J. Souza and D.M. Carraro
The identification of alternatively spliced transcripts has contributed to a better comprehension of developmental mechanisms, tissue-specific physiological processes and human diseases. Polymerase chain reaction amplification of alternatively spliced variants commonly leads to the formation of heteroduplexes as a result of ba.. Read More»
- Genet. Mol. Res. 7(3):
- vol7-3x-meeting012
- DOI:
- 10.4238/vol7-3x-meeting012
Balanced reciprocal translocation at amniocentesis: cytogenetic detection and implications for genetic counseling
Authors: H.G. Zhang, X.Y. Zhang, H.Y. Zhang, T. Tian, S.B. Xu and R.Z. Liu
Balanced translocation is a common structural chromosomal rearrangement in humans. Carriers can be phenotypically normal but have an increased risk of pregnancy loss, fetal death, and the transmission of chromosomal abnormalities to their offspring. Existing prenatal screening technologies and diagnostic procedures fail to detect balanced translocation, s.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038556
- DOI:
- 10.4238/gmr.15038556
Number of repetitions for evaluating technological traits in cotton genotypes
Authors: L.P. Carvalho, F.J.C. Farias, C.L. Morello, J.I.S. Rodrigues and P.E. Teodoro
With the changes in spinning technology, technological cotton traits, such as fiber length, fiber uniformity, fiber strength, fineness, fiber maturity, percentage of fibers, and short fiber index, are of great importance for selecting cotton genotypes. However, for accurate discrimination of genotypes, it is important that these traits are evaluated with .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038557
- DOI:
- 10.4238/gmr.15038557
Testing the performance of automated annotation of ESTs with the Kegg Orthology (KO) database demonstrates lack of completeness of clusters
Authors: G.R. Fernandes, M.A. Mudado and J.M. Ortega
The KEGG Orthology (KO) database was tested as a source for automated annotation of expressed sequence tags (ESTs). We used a control experiment where every EST was assigned to its cognate protein, and an annotation experiment where the ESTs were annotated by proteins from other organisms. Analyzing the results, we could assig.. Read More»
- Genet. Mol. Res. 7(3):
- vol7-3x-meeting011
- DOI:
- 10.4238/vol7-3x-meeting011
Genetic and epigenetic diversity and structure of Phragmites australis from local habitats of the Songnen Prairie using amplified fragment length polymorphism markers
Authors: T. Qiu, L.L. Jiang and Y.F. Yang
The genetic and epigenetic diversity and structure of naturally occurring Phragmites australis populations occupying two different habitats on a small spatial scale in the Songnen Prairie in northeastern China were investigated by assessing amplified fragment length polymorphisms (AFLPs) and methylation-sensitive amplified polymorphisms (MSAPs) through fl.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038585
- DOI:
- 10.4238/gmr.15038585