Comparison of methods for high quantity and quality genomic DNA extraction from raw cow milk
Authors: T. Usman, Y. Yu, C. Liu, Z. Fan and Y. Wang
Isolation of sufficient quantities of high quality DNA is a prerequisite for molecular studies. Milk somatic cells can be used; however, inhibitors such as fats and proteins make milk a difficult medium for extracting large amounts of quality DNA. We optimized, evaluated and compared three methods, Modified Nucleospin Blood Ki.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.29.10
- DOI:
- 10.4238/2014.April.29.10
Adjacent SNPs in the transcriptional regulatory region of the FADS2 gene associated with fatty acid and growth traits in chickens
Authors: S.K. Zhu, Y.D. Tian, S. Zhang, Q.X. Chen, Q.Y.Wang,R.L. Han and X.T. Kang
Delta-6 fatty acid desaturases are rate-limiting desaturases involved in metabolic processes of fatty acids, and they are encoded by the FADS2 gene. In the current study, an F2 resource population of Gushi chickens crossed with Anak broilers was used to investigate the genetic effects of the chicken FADS2. Two adjacent single .. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.29.11
- DOI:
- 10.4238/2014.April.29.11
The first assess of the haplotypes from COI gene sequences in species of spittlebugs (Cicadomorpha: Hemiptera) and aquatic true bugs (Gerromorpha and Nepomorpha: Hemiptera) in Brazil
Authors: M.M.U. Castanhole1, S.R.C. Marchesin2, L.L.V. Pereira1, F.F.F. Moreira3, J.F. Barbosa3, J.R. Val�©rio4 and M.M. Itoyama1
We made the first analysis of the COI gene sequences of 22 species of spittlebugs and aquatic true bugs sampled in São Paulo State (Brazil) and used this information to determine the variability within these groups. Considering each codon position, we observed that the third base was the most variable, and the first base was the most conserved. Amo.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.12
- DOI:
- 10.4238/2013.November.7.12
Effect of secondary lymphoid tissue chemokine suppression on experimental ulcerative colitis in mice
Authors: H. Zhang, X. Zhang, X. Ding, W. Cao, L. Qu and G. Zhou
The secondary lymphoid tissue chemokine (CCL21) is closely associated with lymphoid homing and anti-tumor immune responses. CCL21 also has a chemotactic effect on intestinal lymphocytes. This study mainly focused on CCL21 expression in experimental ulcerative colitis and on the effects of CCL21 suppression on this disease in m.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.12
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.12
Transcriptional regulation of CD4 gene expression in porcine kidney epithelial cells by virus-like double-stranded RNA and DNA methyltransferase inhibitor
Authors: X.S. Wang, H. Ao, L.W. Zhai, L.J. Bai, W.Y. He, C.D. Wang, Y. Yu
The effects of virus-like double-stranded RNA (dsRNA, PolyI:C) and DNA methyltransferase inhibitor (Aza-CdR) on CD4 gene expression were investigated in a porcine kidney cell line (PK15). We found that expression levels of TLR3 and IFNα were significantly upregulated by PolyI:C, compared to the untreated PK15 cells, whic.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.13
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.13
miR-499A>G rs3746444 and miR-146aG>C expression and hepatocellular carcinoma risk in the Chinese population
Authors: Y.F. Shan1, Y.H. Huang1, Z.K. Chen1, K.T. Huang1, M.T. Zhou1, H.Q. Shi1, Q.T. Song1, Z.P. Yu1, A.M. Deng2 and Q.Y. Zhang1
We conducted a case-control study of a possible association of miR-499A>G rs3746444 and miR-146aG>C rs2910164 with risk of hepatocellular carcinoma. Samples from 172 hepatocellular carcinoma patients and 185 cancer-free controls were collected from October 2008 to December 2011. PCR-RFLP analysis was performed to determine the polymorphisms in each .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.11
- DOI:
- 10.4238/2013.November.7.11
Sequence variation in the Toxoplasma gondii eIF4A gene among strains from different hosts and geographical locations
Authors: J. Chen, S.F. Fang, D.H. Zhou, Z.Y. Li, G.H. Liu and X.Q. Zhu
Toxoplasma gondii is an opportunistic protozoan parasite that infects a wide range of animals, including humans. The T. gondii eukaryotic translation initiation factor 4A (eIF4A) protein is expressed in the tachyzoite, but its expression is markedly downregulated in the bradyzoite, and it is therefore considered to be associat.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.14
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.14
Study of candidate genes for dyslexia in Brazilian individuals
Authors: M.C.C.M. Svidnicki1, C.A. Salgado2, R.F. Lima2, S.M. Ciasca2, R. Secolin3, M.C.A. Pomilio4, P.A. Junqueira4, M.S. Pinto4, M.M. Pereira4 and E.L. Sartorato1
Dyslexia or reading disability (RD) is the most common childhood learning disorder and a significantly heritable trait. Many recent studies have investigated the genetic basis of dyslexia, and several candidate genes have been proposed. Among these, DCDC2 and KIAA0319 have emerged as the strongest candidate genes for dyslexia; however studies have not pro.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.10
- DOI:
- 10.4238/2013.November.7.10
A novel nonsense mutation in the sedlin gene (SEDL) causes severe spondyloepiphyseal dysplasia tarda in a five-generation Chinese pedigree
Authors: X.Y. Xia, J. Yu, W.W. Li, N. Li, Q.Y. Wu , X. Zhou, Y.X. Cui and X.J. Li
Spondyloepiphyseal dysplasia tarda (SEDT) is an X-linked recessive osteochondrodysplasia characterized by disproportionately short stature and degenerative joint disease. The objective of this study was to describe a novel nonsense mutation in the sedlin gene (SEDL) causing severe SEDT in a large Chinese pedigree. The clinical.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.15
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.15
ADAMTS14 gene polymorphism associated with knee osteoarthritis in Thai women
Authors: T. Poonpet1,2, S. Honsawek3, N. Tammachote4, S. Kanitnate4,R. Tammachote2
Osteoarthritis (OA) is the most prevalent form of arthritis in the elderly. This disease is characterized by breakdown and loss of articular cartilage due to genetic, mechanical and environmental factors. Although the pathophysiology of OA is not completely known, several candidate genes have been reported to be associated with OA susceptibility. We asses.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.November.7.5
- DOI:
- 10.4238/2013.November.7.5