Relationship between UV-irradiated HaCaT cell cytokines and Th1/Th2 imbalance
Authors: H.Y. Li, F.R. Zhang and D.Q. Deng
We have previously found that an imbalance of Tc1/Tc2 T cell subtypes in vivo impacts the development of photodermatitis. The aim of this study was to investigate the relationship between cytokines derived from keratinocytes exposure to UV and the imbalance of Th subgroups. We used different doses of UVA and UVB to irradiate HaCaT cells. Twelve hours afte.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.5
- DOI:
- 10.4238/2015.July.17.5
Expression of genes encoding cellulolytic enzymes in some Aspergillus species
Authors: M.A. Mahmoud, M.R. Al-Othman, A.R.M. Abd-El-Aziz, H.A. Metwaly and H.A. Mohamed
Fermentation is an important industrial process for microbial metabolite development and has wide applications in various fields. Aspergillus is the most important genus of fungi used for the production of microbial enzymes such as cellulase. The Aspergillus genome encodes various cellulolytic enzymes. In this study, we assaye.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048913
- DOI:
- 10.4238/gmr15048913
Mining SNPs from EST sequences using filters and ensemble classifiers
Authors: J. Wang, Q. Zou and M.Z. Guo
Abundant single nucleotide polymorphisms (SNPs) provide the most complete information for genome-wide association studies. However, due to the bottleneck of manual discovery of putative SNPs and the inaccessibility of the original sequencing reads, it is essential to develop a more efficient and accurate computational method f.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr765
- DOI:
- 10.4238/vol9-2gmr765
Stimulation of dendritic cell maturation and induction of apoptosis in lymphoma cells by a stable lectin from buckwheat seeds
Authors: C.Z. Bai, H.J. Ji, M.L. Feng, X.L. Hao, Q.M. Zhong, X.D. Cui and Z.H. Wang
The present study aims to purify and characterize lectin from tartary buckwheat seeds and study its properties as well as biological activities to determine its possible biomedical applications in promoting maturation and proliferation of peripheral blood DCs derived from healthy donors and to study the effect of inducing apoptosis in human leukemia U937 .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.3
- DOI:
- 10.4238/2015.March.27.3
Characterization of FeDREB1 promoter involved in cold- and drought-inducible expression from common buckwheat (Fagopyrum esculentum)
Authors: Z.W. Fang, X.Y. Xu, J.F. Gao, P.K. Wang, Z.X. Liu and B.L. Feng
C-repeat-binding factor (CBF)/dehydration-responsive element (DREB) transcription factors play key roles in plant stress responses. However, little information is available on the regulation of CBF/DREB expression. In this study, we isolated and characterized the FeDREB1 promoter sequence from the common buckwheat accession Xinong 9976. To identify the up.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.7
- DOI:
- 10.4238/2015.July.17.7
Reduced miRNA-218 expression in pancreatic cancer patients as a predictor of poor prognosis
Authors: B.-S. Li, H. Liu and W.-L. Yang
microRNA-218 (miR-218) is a vertebrate-specific miRNA that plays a crucial role in tumorigenesis and tumor progression. This study analyzed the miR-218 expression level and clinical significance in pancreatic cancer. One hundred and seven pairs of pancreatic cancer and adjacent normal tissues were analyzed by quantitative reverse-transcriptase polymerase .. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015
Serious pulmonary infection in a splenectomized patient with adult type 1 Gaucher disease
Authors: Y. Zhang, Y.F. Mao and J.M. Du
A 49-year-old man with a history of Gaucher disease type 1, resulting in serious splenomegaly and eating disorder, was referred to our department and underwent a splenectomy under general anesthesia. Gaucher disease is very rare, and its first signs are unexplained splenomegaly and hypersplenism. On preoperative examination, the patient’s platelet c.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.13.13
- DOI:
- http://dx.doi.org/10.4238/2015.April.13.13
Complete chloroplast genome sequence of cultivated Morus L. species
Authors: Q.L. Li, J.Z. Guo, N. Yan and C.C. Li
The complete chloroplast genome (cpDNA) sequences of two cultivated species of Morus L. (Morus atropurpurea and Morus multicaulis) are reported and reconstructed in this study, and were compared with that of wild Morus mongolica. In M. atropurpurea, the circular genome is 159,113 bp in size and comprises two identical inverted.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048906
- DOI:
- 10.4238/gmr15048906
Amplified fragment length polymorphism analysis of grapevine rootstock genotypes in Turkey
Authors: A. Erg�¼l, S. Aras and G. S�¶ylemezo��lu
Amplified fragment length polymorphism (AFLP) technique was used to assess genetic relationships among 20 grapevine rootstocks in Turkey. Discrimination of the rootstocks with 10 primer combinations yielded 1366 bands on AFLP gels; 65% of them were polymorphic. The rootstocks revealed two main clusters; one of them comprised t.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr766
- DOI:
- 10.4238/vol9-2gmr766
Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora
Authors: C.A.F. Melo, G.S. Silva and M.M. Souza
The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC1) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prep.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.4
- DOI:
- 10.4238/2015.March.27.4