Evaluation of genetic variability in in vitro sodium azide-induced Dendrobium �Earsakul� mutants
Authors: A. Wannajindaporn, O. Poolsawat, W. Chaowiset and P.A. Tantasawat
In vitro mutagenesis of Dendrobium ‘Earsakul’ was carried out by incubating the protocorm-like bodies in 0-5 mM sodium azide for 1 h. Twenty-eight putative mutants were evaluated for genetic variability compared to untreated control plants using inter-simple sequence repeat (ISSR) analysis. Polymo.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.12
- DOI:
- 10.4238/2014.July.24.12
Clinical three-dimensional conformal radiotherapy research using repeated computed tomography scans for field reduction in older non-small-cell lung cancer patients
Authors: Z.Z. Fu, T. Gu, S.H. Zhang, X.Y. Cao, Q.H. Zhang, Y.Q. Zhang, H.X. Hua, S. Yang, P. Li and Z. Zhang
This study investigated the curative and toxic effects of three-dimensional conformal radiotherapy (3D-CRT), using repeated CT scans for field reduction in older non-small-cell lung cancer (NSCLC) patients. 3D-CRT was administered to 36 older patients with NSCLC, and irradiation fields included the primary lesion and metastati.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.13
- DOI:
- 10.4238/2014.July.24.13
Salicornia europaea L. Na+/H+ antiporter gene improves salt tolerance in transgenic alfalfa (Medicago sativa L.)
Authors: L.Q. Zhang, Y.D. Niu*, H. Huridu, J.F. Hao, Z. Qi and A. Hasi
In order to obtain a salt-tolerant perennial alfalfa (Medicago sativa L.), we transferred the halophyte Salicornia europaea L. Na+/H+ antiporter gene, SeNHX1, to alfalfa by using the Agrobacterium-mediated transformation method. The transformants were confirmed by both PCR and RT-PCR analyse.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.14
- DOI:
- 10.4238/2014.July.24.14
Limited open reduction is better for simple- distal tibial shaft fractures than minimally invasive plate osteosynthesis
Authors: Q. Li, B.F. Zeng, C.F. Luo, S. Song, C.Q. Zhang and W.Q. Kong
The aim of this study was to compare the effects and indications of minimally invasive plate osteosynthesis (MIPO) and limited open reduction (LOR) for managing distal tibial shaft fractures. A total of 79 cases of distal tibial shaft fractures were treated surgically in our trauma center. The 79 fracture cases were classified.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.15
- DOI:
- 10.4238/2014.July.24.15
Expression of the porcine lipoic acid synthase (LIAS) gene in Escherichia coli
Authors: W.F. Lu, J.J. Cao, Y.J. Guo, K. Zhong, G.M. Zha, L.F. Wang and G.Y. Yang
Lipoic acid synthase, which exists primarily in mitochondria, participates in the biosynthesis of intrinsic lipoic acid. The lipoic acid synthase gene in pig is known as LIAS. To further investigate the biological functions of the protein that is encoded by LIAS, we cloned the open read frame of porcine LIAS (GenBank No. JN797612.1) into.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.16
- DOI:
- 10.4238/2014.July.24.16
Genetic variation in and spatial structure of natural populations of Dipterocarpus alatus (Dipterocarpaceae) determined using single sequence repeat markers
Authors: N.M. Tam, V.D. Duy, N.M. Duc, V.D. Giap and B.T.T. Xuan
Dipterocarpus alatus (Dipterocarpaceae) is widely distributed in lowland forests in central and southern Vietnam, Cambodia, Laos, Myanmar, Philippines, Thailand, and India. Due to over-exploitation and habitat destruction, the species is now threatened. The genetic variation within and among populations of D. alat.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.17
- DOI:
- 10.4238/2014.July.24.17
Vitamin D receptor genetic variants are associated with susceptibility of gallbladder adenocarcinoma in a Chinese cohort
Authors: Z. Li, W.T. Yuan, S.J. Ning and S.J. Zhang
The aim of this study was to test for the possible association between vitamin D receptor (VDR) genetic variants and susceptibility to gallbladder cancer (GBC). A total of 291 GBC cases were recruited and 396 gender- and age-matched healthy volunteers were enrolled as controls. The VDR gene polymorphisms were determined in all.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.18
- DOI:
- 10.4238/2014.July.24.18
Alkaline phosphatase activity in salivary gland cells of Rhodnius neglectus and R. prolixus (Hemiptera, Triatominae)
Authors: A.P.M. Lima-Oliveira, K.C.C. Alevi, A.C.B. Anh�ª and M.T.V. Azeredo-Oliveira
Alkaline phosphatase activity was detected in salivary gland cells of the Rhodnius neglectus Lent, 1954, and R. prolixus Stal, 1859, vectors of Trypanosoma cruzi Chagas, 1909 (etiological agent of Chagas disease) and T. rangeli Tejera, 1920 (pathogenic to insect). The Gomori technique was used to demonstrate alkaline phosphatase activity. Alkaline phospha.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038339
- DOI:
- 10.4238/gmr.15038339
A novel NF1 frame-shift mutation (c.702_703delGT) in a Chinese family with neurofibromatosis type 1
Authors: S.P. Cai, N. Fan, J. Chen, Z.L. Xia, Y. Wang, X.M. Zhou, Y. Yin, T.L. Wen, Q.J. Xia, X.Y. Liu and H.Y. Wang
This study aimed to characterize the clinical features of a Chinese pedigree with neurofibromatosis type 1 (NF1) and to identify mutations in the NF1 gene. In this three-generation family containing 8 members, 5 had been diagnosed with NF1 and the others were asymptomatic. All members of the family underwent complete medical e.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.19
- DOI:
- 10.4238/2014.July.24.19
Cloning and characterization of the UBC gene from lotus (Nelumbo nucifera)
Authors: Y. Diao, G.L. Li, A.Q. Yu, X.W. Zheng, K.Q. Xie, Y.W. Wang, M.Q. Zhou, J. Ming and Z.L. Hu
Protein ubiquitination is extensively involved in the regulation of a considerable number of physiological processes in plant cells. E2 (ubiquitin-conjugating enzyme, UBC), one of the essential enzymes of eukaryotic ubiquitination, catalyzes protein ubiquitination together with E1 and E3. In this study, we cloned four full-length cDNANnUBCs of Nelumbo nuc.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038341
- DOI:
- 10.4238/gmr.15038341